Interpenetrating Networks for Delivery

SystemsClient: Professor John W. Kao

Advisor: Professor Kristyn Masters

Claire FlanaganAshley Huth

Max MichalskiAdam Rieves

Overview

• Problem Statement

• Background Information

• Design Constraints

• Design Approaches

• Preliminary Data

• Conclusions

Problem Statement

• Our goal is to create a novel delivery mechanism to reconstitute the components of an interpenetrating network (IPN).

Interpenetrating Network (IPN) Covalently Linked

Therapeutic(s) and/orCell Adhesion Ligands

Bifunctional PEG Linkers

Soluble Therapeutic(s)

BiodegradableGelatin Backbone

PEG-diacrylate(2-3.4 kDa )

Solution(drugs +matrix component)

in situ UV curing

*Kao, W.J

Clinical Applications

•Issues •Uneven administration•Reconstitution Method

•Heat•Time

•Shelf life

IPNConventional Dressings

Irregular Wound

•Benefits •Moist healing environment•Conforms to irregular wounds•Covers large surface areas•Delivers drug cocktails•Very biocompatible

*Kao, W.J.

ingredients w/drug(s) in onecontainer

Mix

Ideal Clinical

Administration

11

Cover55

Shake22

33 Spraywith a controlled distribution over irregular wound surface

44 Curein 30 sec to obtain a rubbery film

66 Sustained Releasewhile the IPN biodegrades

Day

7Day

3Day

1

77 Clean

*Kao, W.J

Design Constraints • Clinically applicable

• No special equipment required • Simple reconstitution methods

• Administration via spray bottle

• Completely reconstitutes

• Meets viscosity requirements

• Cure time < 60 seconds

• Improves Shelf Life

Approach 1: Heating Element

•Exothermic Reaction

•Resistive Element

•Pros•Feasibility•Viscosity•Passive Procedure

•Cons•Cost•Client Preference•Safety

Approach 2: Research•Alter pH•Add Surfactants•Try Buffer Varieties

•Pros•Client Preference•Reconstitution Time•Cost

•Cons•Feasibility•Safety•Active Procedure

Design MatrixCriteria Weight Heating Element ResearchClient Preference 15 4 15Feasibility 15 12 8Viscosity 15 10 7Reconstitution Time 10 7 9Safety 10 6 6Cure Time 10 9 9Human Factors 10 8 7Sterility 5 5 5Shelf Life 5 5 4Cost 5 1 4TOTAL 100 67 74

Preliminary Data

Weight % Gelatin (g/mL) Dissolution10 Yes15 Yes20 No25 No

**Water at 60 ºC

Preliminary DataInitial pH Final pH Dissolved

14 14 +11 10 + / -

9.4 8.3 + / -9.1 8 + / -

7 7.5 -8.2 7.5 + / -

5 6 + / -4 5 + / -

3.1 4.5 + / -2.2 3 +

1 1 +

**All solutions at room temperature

**Final solution 10% gelatin

Conclusions

• We hope to further the clinical applications of IPNs

• Future Work• Continue literature research

• Gather data

• Test complete IPNs

• Reconsider design approach

Questions