Human cells stained with an antibody for the Tubulin protein

Image from Molecular Probes

GFP-RAS2 in living Saccharomyces cerevisiae

Images from Roger Tsein’s Lab

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

Making a Fusion Protein:

Yak1 Gene:DNA: AAAGATGAATCAG ... ACATCGTGACCTA...

Protein: N-Met-Asn-Gln-…-Thr-Ser-C

GFP Gene:DNA: GCAAATGAGTAAA ... CTGAAATAAGTAC...

Protein: N-Met-Ser-Lys-… -Leu-Lys-C

Fusion Gene: AAAGATGAATCAG ... ACATCGATGAGTAAA ... CTGAAATAATGACCTA...

Fusion Protein:N-Met-Asn-Gln-…-Thr-Ser-Met-Ser-Lys-… -Leu-Lys-C

Yak1

GFP

N

C