HPLC Bulk Property Detectors Refractive Index Detector.

HPLC Bulk Property Detectors

Refractive Index Detector

Plus:1) Measures a bulk property2) Nearly Universal (different RI than mobile phase)3) Comparable response for different analytes4) Detects species with no chromophores

Minus:1) Temperature dependent2) Poor sensitivity (LOD ≈ 100 ng)3) No gradient elution

Evaporative Light Scattering Detector

Solid state laser

Plus:1) Measures a bulk property2) Nearly Universal (must be non-volatile)3) Does not detect liquids (gradients are ok)4) Detects species with no chromophores

Minus:1) Signal not linear with concentration2) Fair sensitivity (LOD ≈ 1 ng)3) No salts or buffers in mobile phase

Evaporative Light Scattering Detector

Conductivity Detector

Plus:1) Measures a bulk property2) Nearly Universal (must be ionic)3) Common for Ion Exchange LC4) Detects species with no chromophores5) Simple, robust

Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) No salts or buffers in mobile phase3) Gradients a challenge

Conductivity Detector

HPLC Specific Property Detectors

Conventional UV-Vis Absorption Detector

Hg PenLamp

254 nm

Photodiode 2

Photodiode 1

Multi-wavelength UV-Vis Absorption Detector

DeuteriumLamp

PhotodiodeArray

Plus:1) Measures a specific property2) Nearly Universal (must have chromophore)3) Most common of all detectors (~75%)4) Potential to provide qualitative info.5) Simple, robust

Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) Expensive with PDA, limited with Hg Lamp3) Misses some important analytes

Fluorescence Detector

Plus:1) Measures a specific property2) Highly Selective (must fluoresce)3) Second most common of all detectors (~15%)4) High sensitivity (LOD ≈ 0.01 ng)5) Can interrogate very small volumes

Minus:1) Not Universal2) Limited Applications

Electrochemical Detector

Plus:1) Measures a specific property2) Highly Selective (depends on reduction potential)3) High sensitivity (LOD ≈ 0.01 ng)

Minus:1) Not Universal2) Must have electrolyte in mobile phase3) Mobile phase must be aqueous4) Gradients not possible

Electrochemical Detector

HPLC Element Specific Detectors

ICP Detector Detector

Plus:1) Can be Highly Selective2) Can be nearly Universal3) Can have single sensitivity for all analytes

Minus:1) $$$2) Mobile phase must be aqueous4) Mobile phase must not contain element of interest

ICP Detector Detector

HPLC Element Specific Detectors

UV-Vis detection of sugars in juice

ICP Carbon detection of sugars in juice

ICP Carbon detection of sugars in orange juice

ICP Carbon detection of amino acids

Tag analytes with fluorophoreBefore or After Separation

HPLC Derivatization Detectors

Tag analytes with fluorophoreBefore or After Separation

HPLC Derivatization Detectors

Detect the change in a constant signalas the analyte passes

HPLC Indirect Detectors

Hollow Cathode Detector

Recent HPLC Detector

HPLC Bulk Property Detectors Refractive Index Detector.

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Transcript of HPLC Bulk Property Detectors Refractive Index Detector.

Comparison of Competitor Refractive Index Detectors.

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