Genetic variants ofinfluenzaAH1N1pdm09virusinSouthernMexico

PhD.GuadalupeAyoraTalaveraUniversidadAutónomadeYucatán

MISMSNov7-10,2016

Nuevo Leon

Yucatan

Federal District

Figure 1. Location of the Mexican states selected for the analyses. Nuevo Leon, the Fedral District and Yucatan are shaded in gray.

EpidemiologyofinfluenzainYucatan,Mexico

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pdm09Nationawide TotalInfYucatan pdm09Yucatan

GeneticvariantsoftheAH1N1pdm09virusin2013

HANAA/Yuc/66/2013 MayA/Yuc/79/2013 JuneA/Yuc/80/2013 JuneA/Yuc/81/2013 JuneA/Yuc/82/2013 JuneA/Yuc/116/2013 JulyA/Yuc/124/2013 JulyA/Yuc/128/2013 JulyA/Yuc/133/2013 JulyA/Yuc/134/2013 JulyA/Yuc/135/2013 JulyA/Yuc/137/2013 JulyA/Yuc/138/2013 JulyA/Yuc/141/2013 JulyA/Yuc/150/2013 JulyA/Yuc/152/2013 JulyA/Yuc/157/2013 JulyA/Yuc/214/2013 AugustA/Yuc/243/2013 AugustA/Yuc/250/2013 AugustA/Yuc/260/2013 AugustA/Yuc/286/2013 SeptemberA/Yuc/293/2013 SetemberA/Yuc/317/2013 SeptemberA/Yuc/329/2013 SeptemberA/Yuc/374/2013 October

A141TS162INone

A/Yuc/66/2013 MayA/Yuc/79/2013 JuneA/Yuc/80/2013 JuneA/Yuc/81/2013 JuneA/Yuc/82/2013 JuneA/Yuc/83/2013 JuneA/Yuc/86/2013 JuneA/Yuc/97/2013 JuneA/Yuc/101/2013 JuneA/Yuc/102/2013 JuneA/Yuc/104/2013 JuneA/Yuc/113/2013 JulyA/Yuc/116/2013 JulyA/Yuc/123/2013 JulyA/Yuc/124/2013 JulyA/Yuc/128/2013 JulyA/Yuc/133/2013 JulyA/Yuc/134/2013 JulyA/Yuc/135/2013 JulyA/Yuc/137/2013 JulyA/Yuc/138/2013 JulyA/Yuc/141/2013 JulyA/Yuc/150/2013 JulyA/Yuc/152/2013 JulyA/Yuc/157/2013 JulyA/Yuc/214/2013 AugustA/Yuc/243/2013 AugustA/Yuc/250/2013 AugustA/Yuc/260/2013 AugustA/Yuc/286/2013 SeptemberA/Yuc/293/2013 SeptemberA/Yuc/317/2013 SeptemberA/Yuc/329/2013 SeptemberA/Yuc/346/2013 SeptemberA/Yuc/374/2013 October

N341SL206S+N341SI321VL206SI34V+K432ENone

• HA Fig. 2

• NAFig. 4

Fig. 1

Antigenic Site CaConsideredinducecross-

reactiveprotection

Antigenic Site SaConsidered inducestrain-

specific protection

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A(H1N1)pdm09 A(H3N2) Infuenza B

- TheA(H1N1)pdm09viruswasunderstrongselectivepressuredueeitherto(1)theco-circulationofinfluenzaH3N2viruses,phenomenonthatwasnotobservedsincetheemergencyofthenovelvirusin2009,and/orto(2)thehostimmunepressure.

- ThesubstitutionA141Tisnearthereceptorbindingsite,iftheaminoacidchangeaffectedthereceptoraffinityofthevirus,thenitattainedacompensatorymutationinNA–N341S,inordertosustainvirustransmission.

- However,theshortperiodofcirculationoftheseviralvariantssuggeststhattheycouldhavebeenlesstransmissibleandunabletopersistoverthedominantstrain.Stilltheviruswasunderstrongselectivepressure.

A141T

S162I• Toovercomethis,asecondchangeinadifferentsitewasacquired,accompaniedinsomevirusesbyanovelmutationintheNA- L206S,withunknownproperties.

• Basedonouranalysisandthelowfrequenciesatwhichthesemutationshaveoccurredworldwide(<1%),suggestnoneofthesemutationswerefixedonthepopulationandtheselectivepressureofthevaccinationcampaignforthe2013-2014seasonmighthaveproducedviralextinctionunderabottleneckevent.

• Since2014,viruseshavemutatedatS162N– glycosilation site.

Submitted to PLosOne

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