General Microbiology Laboratory

(Introduction and safety roles )

Microbiology:

is the study of microscopic organisms(include all the bacteria and archaea and almost all the protozoa.

They also include some fungi, algae, and certain animals, such as rotifers.

Some microbiologists also classify viruses as microorganisms, but others consider these as nonliving

Application of microbiology: Food products:

Industrial products :

Environmental application:-• waste water treatment.• Bioremediation(oil spills)• Degradation of heavy metal • Biofuel• Bio control• Pesticide and agriculture product

Pharmaceutical applicants:-• Antimicrobial• Recombinant hormones

Diagnostic and others.

• Wear a lab coat in lab.

• No eating or drinking during lab.• Foot wear that completely covers the foot

is required

Thoroughly wash your hands with soap and water before and after lab.

Clean the lab bench

with disinfectant before and after lab

Keep the lab bench free of unnecessary material

• Dispose of all contaminated materials in autoclave bag.

• Immediately report all accidents and spills to the instructor.

• Keep alcohol from the flame.

Continue.…

ANTISEPTIC TECHNIQUES:-

Sterilization: Is the process of destroying all living organisms.

Disinfection: Is the process of destroying (killing)unwanted (pathogenic) organisms.

Antiseptic : is a chemical disinfection of living tissue (skin)Ex. Wounds treatment .

Methods of antiseptic techniques:

1. Heat:A . dry heat (oven , direct flame )B . Moist heat autoclave.

Chemicals (alcohol , color , detergent).

Radiation (UV,X-Rays).

Filtration .

Some important tools and devices in microbiology lab: