Post on 25-Dec-2015
DNA Binding Proteins
• Histones• Sequence specific• DNA major grove• Homodimeric• Inverted repeats
04/19/23
Negative Control of Transcription
• Operator• Operon
• Enzyme Repression• Repressor• Corepressor
04/19/23
Positive Control of Transcription
• Positive Control
• Activator Protein
• Inducer - Effector
• Operon
• Regulon
04/19/23
Two Component Regulation
• Signal Transduction• Sensor Kinase• Histidine kinase• Response Regulator• 50 in E. coli
04/19/23
Quorum Sensing
• Population density
• Autoinducers
• Biolumunescence
• Virulence
• Chemotaxis
04/19/23
Global Controls
• Catabolite repression• Glucose effect• Diauxic growth• Catabolite Repressor
Protein• cAMP
• Stringent Response• Heat shock proteins
04/19/23
Regulation of Bacterial Development
• Sporulation
• 4 Sigma Factors
• Cellular cannibalism
• Caulobacter Life cycle
04/19/23
RNA-based Regulation
•Noncoding RNA•sRNA•40-400 nucleotides•Antisense RNA•Anti-gene
•Riboswitches04/19/23
Regulation of Gene Expression
Constitutive Isozymes Corepressor
Enzymes Covalent Operator
Regulation of Modification Operon
Enzyme Regulation of Repression
Activity Transcription Repressor
Feedback Enzyme Protein
Inhibition Induction Negative Control
Allosteric Site Inducer Operator04/19/23
Gene Expression 2
Negative DNA Binding Catabolite
Control Proteins Repression
Positive Attenuation Catabolite
Control Leader Activator
Activator Sequence Protein
Protein Attenuator cAMP
Regulon Global Control Quorum
Histones Diauxic Growth Sensing04/19/23
Gene Expression 3
Other Global Signal Antisense
Controls Transduction mRNA
Alternative 2-Component Triple DNA
Sigma Factors Regulation
Proteases Sensor Kinases
Chaperonin Response
Regulator
Chemotaxis04/19/23
Gen Expression
• Constitutive Enzymes
not Regulated• Most Enzymes Can
Have Their Activity Regulated
• Feedback Inhibition
04/19/23
Other Global Controls
• Quorum Sensing
• Alternative Sigma Factors
• Proteases
• Chaperonin
04/19/23