Post on 19-Jan-2016
description
G.007 Hardware open-CAD
• 30 msec move/settle• 42 kbp/sec• 10-60 Gbp/run• $124,000
EM-CCD Camera
Xenon Light
Fluidics
Autofocus
Objective
Stage
3U Imaging2U Sequencing
Hardware: Polonator G.007Nikon, Hamamatsu, Alcott, Prior, Sutter, Bioptechs, PI, Uniblitz, Superlogics
Shendure, Porreca et al. 2005
Danaher, Hamamatsu, Leica $124K with computer & 1 yr serviceContact: kevin.mccarthy@danahermotion.com
Rich Terry et al. 2007
DIY
PersonalGenome.org
36 Flowcells G.007 60M beads per flowcell
PersonalGenome.orgFilling with beads
Gel array, 12M beadsShendure, Porreca et al. 2005
Glass array, 60M beadsKim, Porreca et al. 2007
1.6M reads 12M reads(M. Umbarger)
Higher density & accuracy