Post on 28-Jul-2020
www.poly-an.de
Functionalized Polymer Microparticles
www.poly-an.de
© PolyAn GmbH, November 2017, 6. editioncomposition/layout: TinaBrueser.de
PolyAn is a nanotechnology company specialized in the modification of surfaces using Molecular Surface Engineering (MSE). Since 1996 PolyAn develops and manufactures high-performance consumables for multiplex diagnostics and LifeScience research.
PolyAn is one of the leading producers of functionalized substrates for microarrays. Our wide range of surfaces, substrates and handling tools for microarrays enables our customers to select the most suitable substrate for their specific application.
PolyAn is offering a portfolio of monodisperse PMMA (poly methyl methacrylate) microparticles (beads) for multiplex bead assays, calibration of flow cytometers and calibration of fluorescence imaging systems. PolyAn’s microparticles can be colour encoded with a wide range of fluorescent dyes and functionalized with PolyAn reactive 3D-matrices.
PolyAn´s microplates are used for covalent immobilizing biomolecules that inefficiently coat by passive adsorption. PolyAn offers amine-binding surfaces, providing a convenient method to immobilize biomolecules.
Re-usable calibration tools for fluorescence based detec-tion systems. PolyAn’s calibration slides for cell assays can be used as quality controls in a number of IVD systems for immunology applications.
Molecular Surface Engineering Services: PolyAn is able to equip almost any substrate with our reactive matrices for selective immobilization and antifouling surfaces for the reduction of cell adhesion and unspecific binding, respectively. As part of our Molecular Surface Engineering services, we offer functionalized consumable and substrate materials for OEM applications, which are tailored to specified customer requirements.
Molecular Surface Engineering since 1996
Microarrays
Microparticles
Functionalized Microplates for Immunoassays
Calibrations Tools for Fluorescence Imaging Systems
General Information
General Information
PolyAn offers a portfolio of monodisperse PMMA (poly methyl methacrylate) microparticles for multiplex bead assays, calibration of flow cytometers and calibration of fluorescence imaging systems as well as a wide range of other applications in LifeScience research.
PolyAn’s microparticles can be colour encoded with up to six fluorescent dyes. The fluorophores are directly incorporated into the core of the bead during the microparticle formation. This ensures a homogeneous distribution of the fluoro-phores within the bead and improves the stability of the fluorophores. PolyAn’s microparticles are functionalized using our proprietary Molecular Surface Enginee-ring (MSE) Technology. PolyAn’s reactive matrices are suitable as a platform for a wide range of coupling methods. Our microparticles are characterized by low non-specific adsorption and low aggregation behaviour.
PolyAn’s non-porous microparticles are comprized of a PMMA core with a 3D-surface modification.
Colour/Encoding Fluorophores at different intensitiesUp to six fluorophores in one beadTransparent beads
Molecular Surface Engineering
Size 2–20 µm
Low Aggregation Surface3D-Carboxy, 3D-Alkyne, 3D-Azide,Streptavidin, Neutravidin, Ovalbumin, Protein A/G, Antibodies, Oligonucleotides, Peptides
Uniformity monodisperse, CV 2.5–5 %
Calibrations Tools for Fluorescence Imaging Systems
www.poly-an.de
General Information
Polymer Microparticle CharacteristicsOur polymer microparticles are based on a PMMA core with nanoscale 3D-surface modification.
PolyAn produces microparticles in the range between 2–20 µm. Each bead popula- tion is monodisperse with a maximum Coefficient of Variation (CV) of less than 5 %.
SEM images of different types of microparticles
Using PMMA ensures an excellent optical brilliance and a low autofluorescence compared to other microparticle materials. The refractive index of 1.48 is close to the refractive index of cells (ca. 1.38). Our microparticles have a density of 1.19 g/cm³ and a glass transition temperature (Tg) of about 110 °C. PolyAn uses a biocompatible grade of PMMA.
Imagesources: *Agricultural Research Service, Wikimedia Commons, **Medipan GmbH, ***Universität Potsdam
Escherichia Coli, 1–2 µm* HEp2-Cell, 10 µm** Macrophage, 20 µm***
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particle size distribution [µm]
Fluorescence encoding Tool BoxWith PolyAn’s production process the fluorophores are incorporated into the beads during the polymerization process. This ensures a much more homogene-ous distribution of the dyes within the beads when compared to conventional diffusion controlled dyeing processes. Additionally, the fluorophores are caged within the polymeric PMMA matrix and thus less likely to leak-out.
General Information
Confocal Laser Scanning Microscope (CLSM) image, 3D-Z-stack of PolyAn Orange bead*
ExcitationEmission
Peak
Homogenous Distribution of Fluorophores
* Image courtesy of Bundesanstalt für Materialforschung und -prüfung
Available FluorophoresPolyAn uses a wide selection of fluorophores with emissions ranging from the violett to the near-infrared. Additional dyes for specific requirements are available upon request.
425 nmPolyAn Blue 390 nm
PolyAn Plex C 455 nm510 nm
PolyAn Plex R 535 nm565 nm
PolyAn Pink 540 nm565 nm
PolyAn Green 465 nm480 nm
PolyAn Orange 515 nm530 nm
PolyAn Red 560 nm590 nm
300 nm 400 nm 500 nm 600 nm 700 nm 800 nm
PolyAn Red 4 650 nm670 nm
PolyAn Red 5 610 nm690 nm
www.poly-an.de
General Information
Molecular Surface Engineering
By using MSE-technology, a thin polymer shell of a few nanometers, consisting of carboxyl groups, is formed on the surface. This occurs without changing the excellent physical and optical properties of the PMMA-core. Our 3D-Carboxy microparticles are suitable for EDC/EDAC mediated coupling of amine-containing molecules or for ionic interaction.
PolyAn offers the following surfaces for immobilization of DNA, peptides and proteins:
PolyAn’s high-performance polymer microparticles are functionalized with a 3D-surface chemistry comprised of a long-chain polymer with a defined number of reactive groups. In contrast to conventional coating procedures, the reactive polymer is covalently linked to the surface.
Schematic model of the 3D reactive matrix on the core polymer microparticle
Beadsize (core)
Thickness of Functional Layer
Function
2–20 µm
Low Aggregation Matrix
1–10 nm
PMMA (polymethylmethacrylate) microparticles/microspheres
REM: different surface morphologies of carboxylated microspheres
Olympus, 60x: dyed functional surfaces on beads
Unspecific binding and aggregation of biomolecules is reduced by our low aggregation matrix. Our rigorous quality control procedures according to ISO 9001 ensure the constant loading and low batch-to-batch variation necessary for mole-cular diagnostics and pharma screening.
• 3D-Carboxy for EDC/EDAC mediated coupling
• 3D-Alkyne for „click chemistry“
• 3D-Azide for „click chemistry“
• Streptavidin and Neutravidin for coupling of biotinylated biomolecules
• Poly-L-Lysin for attachment of cells and proteins
• Protein A/G for binding of IgG
N
N
N+
HC C
Directed/Oriented Immobilization of Biomolecules
Surfaces for Click ChemistryPolyAn has developed new 3D-Alkyne and 3D-Azide surfaces for oriented and bio-orthogonal coupling of biomolecules:
• Alternative to conventional Streptavidin-Biotin chemistry
• Less unspecific interactions compared to Streptavidin
• No reactions of alkyne with regular biomolecule functionalities, e.g. hydroxy, amino and carboxy
5 µm fluorescent PMMA beads with capture oligonucleotide immobilized via click chemistry
• Hybridization with 6-FAM labelled anti-strandOlympus, 20x: Fluorescence image after hybridization
cat. Cu(I)
N
N
CCH
N
Low Aggregation Matrix
+ Biomolecule
Biomolecule
+strain promotedcycloaddition
Directed coupling of Oligonucleotides and PeptidesAs part of our functionalization services PolyAn is now offering the custom modification of beads with oligonucleotides or peptides.
N
Reactive Group
- Alkyne
- Azide
C CH
N N N+
N
- DBCO
N
NN
N
Biomolecule
Biomolecule
click reaction hybridization
www.poly-an.de
Calibration Tools
Fluorescence Calibration Slides
PolyAn’s calibration slides are designed for the routine calibration of confocal fluorescence microscopes and other fluorescence imaging systems. They are prepared by mounting statistically distributed monodisperse PMMA beads that contain ultra-stable fluorophores onto standard 75 x 25 x 1 mm glass slides. The beads are protected from mechanical stress with a coverglass.
Fluorescence image of a calibration slide (green channel): homogeneous particle distribution, no aggregates.
• Blue emission channel e.g. DAPI
• Green emission channel e.g. FITC, Cy3 ®
• Red emission channel e.g. APC, Cy5 ®
• For the routine calibration of fluorescence microscopes
• For automated fluorescence imaging systems, e.g. scanning cytometry
Available for three different emission wavelengths
Characteristics:
• Monolayer of fluorescent beads on glass slides
• High photostability
• Homogeneous particle size and fluorescence intensity
• Single particles, no particle aggregates and homogeneous, statistical particle distribution
• Excellent slide-to-slide and batch-to-batch reproducibility, CV< 3%
• Long term stability: less than 0.5 % decrease in fluorescence intensity after 1 month at 37°C
• Standard size: 75 x 25 x 1 mm glass slides, alternative formats are available upon request
Photostability: slides mounted with “Green” and “Red” emitting beads were measured multiple times over a period of 50 days. The fluorescence intensity after more than 50 measurements exceeded 97 % of the initial intensity for both dyes, underlining their excellent photostability.
number of runs
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Cy ® is a trademark of Amersham Biosciences Corp.*
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Calibration Tools
PolyAn’s Spectrum Calibration Beads are designed for calibration of flow cytometers and other fluorescence imaging systems. Each color encoded PMMA bead population (peak) contains a mixture of fluorophores that allows performance validation at all wavelengths.
8-peak Spectrum Calibration Beads with increasing
fluorophore content for all channels. One transparent
population (only in FL3 detectable) and 7 fluorescence
encoded populations. Measurement with QA Quantum P
flow cytometer. Excitation laser line at 488 nm.
• Contains a mixture of fluorophores that enable the Spectrum Calibration Beads to be excited at any wavelength from 365 nm to 650 nm.
• Fluorophores are homogeneously encapsulated in the PMMA matrix. Special shell prevents leaching of fluorophores.
• Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels with the same set of particles.
• Set of up to 8 similar size microparticle populations (peaks) with different fluorescence intensities.
Individual packaging, other sizes and alternative fluorescence intensities (peaks) are available upon request. Please contact our customer service for a custom development.
Description
Spectrum Flow Cytometer Calibration Beads, 1 population (peak)
Spectrum Flow Cytometer Calibration Beads, 5 populations (peaks)
Spectrum Flow Cytometer Calibration Beads, 8 populations (peaks)
Product-ID
107 02 006
107 01 006
107 00 006
Key features
1000
100
10
00,1 1 10 100 1000
FL3
SS
C
coun
t
200
160
120
80
40
00,1 1 10 100 1000
FL3
coun
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120
80
40
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FL2
coun
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150
100
50
00,1 1 10 100 1000
FL1
Spectrum Calibration Beads
www.poly-an.de
Slide during detection Focused fluorescence encoded and labelled microparticles
Multiplex Beads for Fluorescence Microscopes
Microparticles for Scanning CytometryFor fluorescence microscopy based detection systems PolyAn has developed serveral sets of multiplex beads that can be distinguished by both different sizes as well as different colour encodings. In order to facilitate detection and reduce the requirements with regards to the optical system, these multiplex beads are larger and have a higher fluorescence intensity.
The AKLIDES detection platform consists of an inverse fluorescence microscope with a motorized scan stage.
Example: AKLIDES® System
The bead populations are distinguished by their fluorescence and size, respectively. In the first step, the beads are focused by a dynamic autofocus (1). Subsequently, the beads are classified and assigned to their bead population (2). In the final step, the ligand fluorescence is detected using a fluorescence label illustrated by the red corona (3).
Classification, assignment and evaluation of multiplex beads The images are detected by a CCD camera. The multi-colour fluorescence image- capture-based system uses pattern recognition algorithms for multiplex testing.
Our PolyAn Plex beads are also available with Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies or oligonucleotides is available upon request.
Colour LabelingProduct-ID Excitation/EmissionPeaksDiameter Surface
Dual Colour
Dual Colour
105 02 011105 02 015
105 31 011105 31 015
420–480 nm / 515–540 nm485–540 nm / 535–570 nm
420–480 nm / 515–540 nm485–540 nm / 535–570 nm
18
18
11/15 µm
11/15 µm
3D-Carboxy LA
Streptavidin
1 3A
A
B
B
C
C
C
2A
A
B
B
C
C
CB
B B
Products
Microparticles for Flow Cytometry
PolyAn Plex bead kits provide a platform for the design of multiplexed suspension arrays that can be run on standard flow cytometers. PolyAn offers a 25-plex (peaks) set of beads that can be distinguished by both different fluorescence intensities of our PolyAn Red4 dye (Excitation: 590–680 nm/Emission: 660–780 nm) as well as three different sizes (3.5 µm, 5 µm and 9 µm).
PolyAn Red4 25 Plex
Single Dye encoded Beads for Multiplex Assays – 25 Plex
Images courtesy of Systems Immunology Lab (Humboldt Universität Berlin)Image courtesy of Quantum Analysis GmbH
***
Colour LabelingProduct-ID Excitation/EmissionPeaksDiameter Surface
Our PolyAn Plex beads are also available with Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
PolyAn Red4PolyAn Red4PolyAn Red4
PolyAn Red4PolyAn Red4PolyAn Red4
106 50 003106 50 005106 50 009
106 52 003106 52 005106 52 009
590–680 nm/660–780 nm 590–680 nm/660–780 nm 590–680 nm/660–780 nm
590–680 nm/660–780 nm 590–680 nm/660–780 nm 590–680 nm/660–780 nm
8107
8107
3.5 µm5 µm9 µm
3.5 µm5 µm9 µm
3D-Carboxy LA3D-Carboxy LA3D-Carboxy LA
StreptavidinStreptavidinStreptavidin
1 2 3 40Red4
SS
C-H
PolyAn Red4 Plex Beads: Detection of 25-plex (three sizes with 7-10 different fluorescence intensities) with BD FACSCanto II *
5
4
3
5
PolyAn Red4 Plex Beads: Detection of 25-plex with Quantum P Flow Cytometer **
0.1 1 10001001010
100
1000S-002
SS
C
FL5
www.poly-an.de
Fluorescence Lifetime Beads
Microparticles for Fluorescence Lifetime Applications
Differences in fluorescence-lifetime are for example used in fluorescence lifetime imaging microscopy (FLIM). FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It is used in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.
Our PolyAn FLT beads are also available with Streptavidin, Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
Images courtesy of Bundesanstalt für Materialforschung und -prüfung (BAM)*
Product-ID
110 00 006
110 20 006
Diameter
6.5 µm
6.9 µm
Surface
3D-Carboxy LA
3D-Carboxy LA
Fluorescence Lifetime
1.6 ns
5.5 ns
FLIM: FLT encoded beads with ligand fluorescence
y / µ
m
0
50
100
150
200
x / µm0 50 100 150 200
T / ns
1
3
4
6
8
9
Histogramm: PolyAn FLT 1NS and 6NS beads measured with a fluorescence lifetime flow cytometer
Decay curves of PolyAn FLT beads
PolyAn FLT Beads
Num
ber
of e
vent
s
0
5
10
15
20
Tint / ns0 1 2 3 4 5 6 7 8 9 10
25
0 10 3020 40Times / ns
100
10-3
10-2
10-1
Inte
nsity
(nor
mal
ized
)
Molecular Surface Engineering Services
Our service: Customized Beads / Bead Populations with individual surface functionalization solutionsAs part of our Molecular Surface Engineering Services, we offer the individual development of beads for specific requirements.
Custom product development is the cornerstone capability from which PolyAn’s family of products evolved. PolyAn has developed a broad repertoire of bead manufacturing capabilities that meet customer specifications with regards to tolerances, bio-compatibility, and assay conditions.
Our scientists partner with our customers to rapidly build prototypes that enable scaled development and manufacturing. As a development partner, PolyAn facilita-tes efficiencies and innovation to maximize your capacities in research and analysis rather than in development and manufacturing. Let us know what you and your company are exploring and we can support you in making that a reality.
Statistically distributed mounted beads for calibration printed beads, i.e. spatially oriented beads
• Unmodified
• Low Aggregation
• Binding
- 3D-Carboxy - 3D-Alkyne - 3D-Azide - Streptavidin / Neutravidin - Poly-L-Lysin - Protein A/G - Antibodies - Peptides - Oligonucleotides
• Immobilized dyes
Shell / Surface
• Transparent
• Fluorescent
- Coustomized colour, excitation / emission - Single dye - Multiple dyes - Customized fluorescence intensity - Fluorescence Lifetime - Multiplex encoding - Calibrated Brightness
Core / Bead
Diameter: 2 – 20 µm Size distribution
www.poly-an.de
Products
PolyAn offers transparent polymer beads with a narrow particle size distribution (CV less than 5%) for particle analysis using flow cytometry or other screening applications.
In addition to the products listed above, PolyAn also produces transparent beads that are functionalized with Neutravidin, 3D-Azide and Protein A/G. PolyAn can custom modify of beads with antibodies, oligonucleotides and peptides, respectively. Please have a look at our website www.poly-an.de for a complete overview of our portfolio.
Transparent PMMA Beads
DescriptionProduct-ID Diameter
105 00 002105 00 005105 00 009105 00 012105 00 016105 00 020
2 µm 5 µm 9 µm12 µm16 µm20 µm
Transparent PMMA BeadsTransparent PMMA BeadsTransparent PMMA BeadsTransparent PMMA BeadsTransparent PMMA BeadsTransparent PMMA Beads
Functionalized transparent PMMA BeadsSurfaceProduct-ID Diameter
105 11 002105 11 005105 11 009105 11 012105 11 016105 11 020
105 21 002105 21 005105 21 009105 21 012105 21 016105 21 020
108 31 002108 31 005108 31 009108 31 012108 31 016108 31 020
2 µm 5 µm 9 µm12 µm16 µm20 µm
2 µm 5 µm 9 µm12 µm16 µm20 µm
2 µm 5 µm 9 µm12 µm16 µm20 µm
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
StreptavidinStreptavidinStreptavidinStreptavidinStreptavidinStreptavidin
3D-Azide3D-Azide3D-Azide3D-Azide3D-Azide3D-Azide
Fluorescence encoded PMMA BeadsPolyAn‘s fluorescent microparticles are available in various sizes, emission spectra and fluorescence intensities. The fluorescent PMMA microparticles are suitable for use in flow cytometry, fluorescence microscopy, phagocytosis studies and cell labelling. They can be used in image based systems as well as in other screening applications.
Please note, that all microparticles can be produced in sizes between 2–20 µm. It is possible to tailor the fluorescence intensity to your specific requirements. PolyAn also produces customized microparticles which incorporate fluorophores for other spectral ranges.
105 89 002105 89 005105 89 009105 89 012
105 60 002105 60 005105 60 009105 60 012
106 70 002106 70 005106 70 009106 70 012
105 40 002105 40 005105 40 009105 40 012
106 00 002106 00 005106 00 009106 00 012
106 10 002106 10 005106 10 009106 10 012
Colour Labeling Product-ID Diameter
PolyAn BluePolyAn BluePolyAn BluePolyAn Blue
PolyAn GreenPolyAn GreenPolyAn GreenPolyAn Green
PolyAn PinkPolyAn PinkPolyAn PinkPolyAn Pink
PolyAn RedPolyAn RedPolyAn RedPolyAn Red
PolyAn Red4PolyAn Red4PolyAn Red4PolyAn Red4
PolyAn Red5PolyAn Red5PolyAn Red5PolyAn Red5
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
Excitation/Emission
350–400 nm/400–480 nm 350–400 nm/400–480 nm350–400 nm/400–480 nm350–400 nm/400–480 nm
415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm
450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm
510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm
590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm
490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm
Products
www.poly-an.de
Products
Functionalized fluorescence encoded BeadsPolyAn offers a wide variety of functionalized, fluorescent particles. They can be used in flow cytometry, image based systems as well as in other applications. The fluorescence intensity of the beads can be tailored to specific applications and read-out systems. We are happy to help you select the right fluorescence intensity for your application.
Please note, that all microparticles can be produced in sizes between 2–20 µm. PolyAn also produces customized microparticles which incorporate fluorophores for other spectral ranges.
Our fluorescence encoded beads are also available with Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies or oligonucleotides is available upon request.
105 91 002105 91 005105 91 009105 91 012
105 60 002105 60 005105 60 009105 60 012
106 72 002106 72 005106 72 009106 72 012
105 45 002105 45 005105 45 009105 45 012
106 02 002106 02 005106 02 009106 02 012
106 11 002106 11 005106 11 009106 11 012
Colour Labeling Product-ID Excitation/EmissionDiameter
PolyAn BluePolyAn BluePolyAn BluePolyAn Blue
PolyAn GreenPolyAn GreenPolyAn GreenPolyAn Green
PolyAn PinkPolyAn PinkPolyAn PinkPolyAn Pink
PolyAn RedPolyAn RedPolyAn RedPolyAn Red
PolyAn Red4PolyAn Red4PolyAn Red4PolyAn Red4
PolyAn Red5PolyAn Red5PolyAn Red5PolyAn Red5
350–400 nm/400–480 nm 350–400 nm/400–480 nm350–400 nm/400–480 nm350–400 nm/400–480 nm
415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm
450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm
510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm
590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm
490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
Surface
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
3D-Carboxy3D-Carboxy 3D-Carboxy3D-Carboxy
3D-Carboxy3D-Carboxy3D-Carboxy3D-Carboxy
Functionalized fluorescence encoded BeadsPolyAn offers a wide variety of functionalized, fluorescent particles. They can be used in flow cytometry, image based systems as well as in other applications. The fluorescence intensity of the beads can be tailored to specific applications and read-out systems. We are happy to help you select the right fluorescence intensity for your application.
Please note, that all microparticles can be produced in sizes between 2–20 µm. PolyAn also produces customized microparticles which incorporate fluorophores for other spectral ranges.
Our fluorescence encoded beads are also available with Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies or oligonucleotides is available upon request.
105 92 002105 92 005105 92 009105 92 012
105 62 002105 62 005105 62 009105 62 012
106 73 002106 73 005106 73 009106 73 012
105 43 002105 43 005105 43 009105 43 012
106 03 002106 03 005106 03 009106 03 012
106 14 002106 14 005106 14 009106 14 012
Colour Labeling Product-ID Excitation/EmissionDiameter
PolyAn BluePolyAn BluePolyAn BluePolyAn Blue
PolyAn GreenPolyAn GreenPolyAn GreenPolyAn Green
PolyAn PinkPolyAn PinkPolyAn PinkPolyAn Pink
PolyAn RedPolyAn RedPolyAn RedPolyAn Red
PolyAn Red4PolyAn Red4PolyAn Red4PolyAn Red4
PolyAn Red5PolyAn Red5PolyAn Red5PolyAn Red5
350–400 nm/400–480 nm 350–400 nm/400–480 nm350–400 nm/400–480 nm350–400 nm/400–480 nm
415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm415–480 nm/470–550 nm
450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm450–565 nm/540–620 nm
510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm510–580 nm/570–630 nm
590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm590–680 nm/660–780 nm
490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm490–680 nm/660–730 nm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
2 µm 5 µm9 µm
12 µm
Surface
StreptavidinStreptavidinStreptavidinStreptavidin
StreptavidinStreptavidinStreptavidinStreptavidin
StreptavidinStreptavidinStreptavidinStreptavidin
StreptavidinStreptavidinStreptavidinStreptavidin
StreptavidinStreptavidinStreptavidinStreptavidin
StreptavidinStreptavidinStreptavidinStreptavidin
Products
www.poly-an.de
Ordering Information
How to place an order
We are looking forward to your telephone orders and technical enquiries at our Customer Service and Technical Service Department Monday-Friday. Office hours for telephone enquiries are 9:00 AM to 6:00 PM (Central European Time).Please mention billing and shipping addresses, product-ID, quantity, your phone number or Email and name.
All prices are Ex-Works PolyAn, Berlin. The beads will be shipped via FedEx, UPS, TNT, DHL or airmail
Terms & conditions PolyAn‘s general terms & conditions apply.
Minimum quantity The beads are packaged in aqueous suspensions with a volume of 1.5 mL, 4 mL and 10 mL, respectively. These are the minimum quantities. Please note, that discounts are available for large order volumes.
Ordering process After placing your order you should receive an order acknowledgement via e-mail within 3 business days. When your beads have been shipped, we will notify you via e-mail to provide you with the shipping information, e.g. tracking number.
Payment terms The full price is payable within 14 days after the date of the invoice.
Shipping and handling
Contact Tel +49 (0)30 912 07 80Fax +49 (0)30 912 07 811Email mail@poly-an.de
PolyAn pursues a policy of continued technical excellence to deliver high quality products and services. Our company is dedicated to product consistency and reliability – providing our customers with highly reproducible consumables for their specific applications. To ensure this PolyAn has successfully implemented the DIN EN ISO 9001 quality management system for all processes. PolyAn’s production facilities also include a class 5 cleanroom.
Quality
Ordering Information
Distributors
Bio-cando inc., TaiwanTel +886 (0)3 211 8079Email bio-cando@promogene.com.tw
AutoMate Scientific, Inc. (USA)Tel +1 (0)510 845 6283 Fax +1 (0)510 280 3795Email info@autom8.com
USA, Canada, Mexico
Singapore, Malaysia, Indonesia
Sciencewerke Pte. Ltd. (Singapore)Tel +65 (0)6777 1045Fax +65 (0)6777 3054Email jason@sciencewerke.com
Taiwan
CytoTechs, inc.Tel +81 29 834 7788Fax +81 29 834 7772Email j.iijima@cytotechs.com
Japan
K.F. Technology SrlTel +39 (0)6 454 34 179Fax +39 (0)6 9725 31 31Email fabrizio@kftechnology.it
Italy
Moshe Stauber Biotec ApplicationsTel +972 (0)8 936 70 01Fax +972 (0)8 936 70 02Email stauber1@zahav.net.il
Israel
PROTEIGENE SARLTel +33 (0)2 32 64 45 45Fax +33 (0)2 32 64 30 72 Email info@proteigene.com
France
Kyongshin Scientific Co., Ltd. Tel +82 (0)2 576 6303Fax +82 (0)2 576 6309 Email lee@kyongshin.co.kr
Korea
BioAlbra Biotecnologia LtdaTel +55 (31) 98815 1070Email info@bioalbra.com
Brazil
www.poly-an.de
PolyAn GmbHRudolf-Baschant-Str. 213086 BerlinGermany
+49 30 912 07 80+49 30 912 07 811mail@poly-an.de
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