Post on 26-Dec-2021
FRAENKEL - CONRAT’S
EXPERIMENT
(DNA as the carrier of genetic
information)
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
DR. UTTAM KUMAR KANP
ASSISTANT PROFESSOR
DEPARTMENT OF BOTANY
NARAJOLE RAJ COLLEGE
DNA is deoxyribo nucleic acid.
DNA contain three main components (1) phosphate groups (2)
five –carbon sugars; and (3) nitrogen – containing bases called
purines [Adenine (A) and guanine (G)] and pyrimidines [
thymine (T) and cytosine (C) ]. Nitrogen base and sugar make a
nucleoside. Phosphate group and a nucleoside make nucleotide.
DNA is composed of two stand of nucleotides. The two strands
are held together by H- bonds .(complementary base pairing)
A pyrimidine in one chain is always paired with a purine in the
other chain.
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
HERSHEY AND
CHASE
GRIFFITH
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
FRAENKEL-CONRAT’S EXPERIMENT They were working with TMV in 1957 to prove that RNA can
act as genetic material.
TMV has a protein capsid and RNA inside.
They separated the protein coat and the RNA and used themto infect tobacco leaves.
When only the separated protein coat was used, no infectionoccurred while only RNA could infect tobacco leaves.
When the separated protein coat and RNA wererecombined ,entire TMV particles were recommitted.
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
•Next , they selected two strains of TMV;TMV-A
(causes mottling of leaves ) and TMV-B (causes wrinkle pattern on leaves),
•The protein and RNA parts of each strain were separated and chimeric TMV
particles were generated.
•The chimeric TMV produced symptoms that were characteristics of the RNA
and not the protein.
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .
THANK YOU
COMPILED AND CIRCULATED BY DR. UTTAM KUMAR KANP, ASSISTANT PROFESSOR,
DEPARTMENT OF BOTANY, NARAJOLE RAJ COLLEGE
BOTANY: SEM-IV, PAPER-C8T: MOLECULAR BIOLOGY, UNIT-1: NUCLEIC ACIDS: CARRIERS OF GENETIC INFORMATION .