Post on 30-Jul-2022
ISO-compliant chromogenic medium
ESIA is the ISO/TS 22964 standard required medium for the detection of Cronobacter spp. in powdered milk and powdered infant formula
Additional reagents required according to this ISO method are available: ESIA Agar, Buffered Peptone Water, mLST broth, TSA agar
Easy-to-use: Easy-to-interpret results: blue-green colonies on a clear background
Convenient protocol “1 bag/1 plate”: ESSB broth formulated to provide optimal growth of Cronobacter spp. while inhibiting interfering flora on ESIA agar
Results in 21 ± 3 hours at 44 ± 1˚C
Chromogenic culture medium for the specific detection
of Cronobacter spp. (including Cronobacter sakazakii) in food samples such as milk powder, powdered infant
formula and food prepared from milk powder
Typical blue-green colonies: Cronobacter spp.
esiaTM
esiaTM
bioMérieux Industry595 Anglum Road
Hazelwood, MO 63042 • U.S.A.
Tel: (800) 634 7656
Fax: (314) 731 8678
bioMérieux Canada, Inc.7815 Henri-Bourassa Boulevard West
St. Laurent (Québec) • Canada H4S 1P7
Tel: (1) 514 336 7321
Fax: (1) 514 807 0015
www.biomerieux-usa.comDU
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> ESIA Medium
20 plates 90mm Ref. AEB520010
> ESIA Agar-Dehydrated
500g Ref. AEB150002
> Other media:
ESSB Broth - Cronobacter spp.6 x 225ml bottles Ref. AEB611448
Modified LST Broth100 x 10ml tubes Ref. AEB110549
Tryptic Soy Agar10 plates 90mm Ref. M1040
Buffered Peptone Water 500g Ref. 46925 100 x 9ml tubes Ref. 421116 x 90ml bottles Ref. 42042 6 x 225ml bottles Ref. 4204310 x 225ml Minibags Ref. 427293 x 3L bags Ref. 42629
Selective isolation agar for Cronobacter spp. (including C. sakazakii) detection in infant milk powders
Alternative method for the detection of Cronobacter spp. according to ESSB/ESIA protocol
Homogenize X g of sampleIn 9 X ml of ESSB Broth
Incubate for 24 ± 2 hours at 37 ± 1˚C
Isolate on ESIA agarIncubate for 21 ± 3 hours at 44 ± 1˚C
Presence of typical colonies (Blue colonies)
Confirmation with biochemicals or VITEK
automated identification system
Absence of typical colonies(Purple colonies)
Absence of Cronobacter spp. in X grams of sample