Post on 18-Jan-2018
description
Enzymes
Amino Acid Basic Structure
Primary Structure
Secondary Structure
Tertiary Structure
Quaternary Structure
Yeast
Sucrase
Human/Bacterial/Bacterial Mg
Catalase- H2O2 -> O2 + H20
Catalase Lab
• Intro:• What is catalase / function/structure • Structure of proteins• Overview of Lab- pH, Temperature, substrate• Organism used in Lab
• Problem- write as a class• Hypothesis- write as a class• Procedure- See Vernier Biology Manual- 6B
» Enzyme Action: Testing Catalase Activity
HOW ENZYMES FUNCTION
• Enzymes speed up the cell’s chemical reactions by lowering energy barriers
– For a chemical reaction to begin• Reactants must absorb some energy, called the
energy of activation
Figure 5.5A
EA barrier
Reactants
Products1 2E
nzym
e
– A protein catalyst called an enzyme• Can decrease the energy of activation needed to
begin a reaction
Figure 5.5B
Reactants
EA withoutenzyme
EA withenzyme
Net changein energy
Products
Ene
rgy
Progress of the reaction
• A specific enzyme catalyzes each cellular reaction– Enzymes have unique three-dimensional
shapes• That determine which chemical reactions occur in
a cell
Figure 5.6
Enzyme(sucrase)Glucose
Fructose
Active site Substrate(sucrose)
H2O
1 Enzyme availablewith empty activesite
2 Substrate binds to enzyme with induced fit
4 Products arereleased
3 Substrate is converted to products
– The catalytic cycle of an enzyme
• 5.7 The cellular environment affects enzyme activity– Temperature, salt concentration, and pH
influence enzyme activity– Some enzymes require nonprotein cofactors
• Such as metal ions or organic molecules called coenzymes
• Enzyme inhibitors block enzyme action– Inhibitors interfere with an enzyme’s activity
– A competitive inhibitor• Takes the place of a substrate in the active site
– A noncompetitive inhibitor• Alters an enzyme’s function by changing its shape
Figure 5.8
Substrate
Enzyme
Active site
Normal binding of substrate
Enzyme inhibition
Noncompetitiveinhibitor
Competitiveinhibitor