Stem Cell PartnersA Regenerative Medicine Company

Stem Cell Partners LLC

A Regenerative Medicine Company

A Regenerative Medicine Company

ENSURA-SEP POINT OF CAREPOINT OF CARECELL WASHING

C t tContact:Lee Buckler

lbuckler@celltherapygroup.com778 278 6311

1

778.278.6311

IntroductionThe Ensura-Sep™ Canister is intended for the washing of a cell suspension at the point of care.washing of a cell suspension at the point of care.

Cell washing and cell concentration are achieved in a single centrifugation step.

Technology is density phase washing withTechnology is density phase washing with proprietary centrifuge canister apparatus.

Potential applications include removal of DMSO, Ficoll, enzymes, or red cell lysing reagents.

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y y g g

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Summary of Cell Washing Method with Ensura-Sep: Slide 1 of 2

Add density blockingEnsura-Sep Tube

Add density blocking reagent to verticalchamber.

Add cell suspension fluid to reservoir chamber.

Pellet cells by centrifugation (no intermingling of fluid phases occurs)

Blocking fl id (bl e) has a densit that is less than cells (red circles)Blocking fluid (blue) has a density that is less than cells (red circles)but greater than cell suspending fluid (yellow).

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Summary of Cell Washing Method with Ensura-Sep: Slide 2 of 2

5. Aspirate depleted fluid suspensionfrom reservoir chamber.

6. Resuspend cells & harvest cells by final aspiration into

syringe. 5

Step 1: Add 2.4 mL of Density Blocking Reagent

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Step 2: Add 10 - 40 mL of cell suspension

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Step 3: Centrifuge (10 min for 2,000 x g)

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Step 4: Empty reservoir chamber

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Step 5: Harvest Cells (flushing to resuspend)

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SummarySummaryEnsura-Sep Cell Canisters enable theEnsura Sep Cell Canisters enable the simple and rapid washing/concentrationof a cell suspension to be achieved in aof a cell suspension to be achieved in a single centrifugation step.

We work with our clients to design custom canisters and reagents to meetcustom canisters and reagents to meet their specific requirements.

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Stem Cell PartnersA Regenerative Medicine Company

Stem Cell Partners LLC

A Regenerative Medicine Company

A Regenerative Medicine Company

ENSURA-SEP

POINT OF CARE

CELL WASHING

Contact:

Lee Bucklerlbuckler@celltherapygroup.com

1

778.278.6311