Post on 13-Oct-2020
DETERMINATION OF ANDROGENIC STEROIDS IN URINE BY GC-MS
Angel Totoricagüena, Silvio D. Barletta and Carlos E. Alli
Sector of Active Substances and Chemical Residues, General Direction of Laboratory - National Service of Health and Agri-Food Quality (SENASA) - ArgentinaContact: atotoric@senasa.gob.ar - sbarletta@senasa.gob.ar - calli@senasa.gob.ar
Introduction
Figure 1. Chemical structures of studied androgens.
Methods
GC-MS Conditions
Talcahuano 1660CP 1640, Martinez, Buenos Aires, Argentina
www.senasa.gob.ar
Samples Preparation
Results
Figure 3. Linearity of MTST and β-BLD with d3-BLD.
Figure 4. Linearity of α-NTT and β-NTT with d3-TST.Figure 2. a) Chromatogram of MTST (RT: 20.167 min); b) Chromatogram of β-BLD and d3-BLD (RT: 21.874 min); c) Chromatogram of α-NTT/ β-NTT and d3-TST (RT: 21.795/22.722 and 23.001).
All results obtained for the studied parameters, linearity, reproducibility and repeatability, selectivity and ruggednnes are in accordance with SENASA Resolution N°138/2002 and its modifying dispositions Nº 125/200 6 and Nº 06/2004, as well as EU Council Directive 2002/657/EC.This method also demonstrates its effectiveness for the identification and quantification of Methyl Boldenone in urine by GC-MS.
Analyte Analytical range
(µg/l)
Average
recovery %
LD o CCα
(µg/l)
CCβ
(µg/l)
α-NTT 0.96 – 7.97 96.2 0.32 0.55
β-NTT 0.56 – 4.51 95.8 0.12 0.26
β-BLD 0.58 – 4.63 100.4 0.11 0.13
MTST 0.99 – 7.88 99.5 0.19 0.34
Conclusions
Table 1. Validation parameters obtained for each androgenic steroid.