+dpy-5

dpy-5

+

unc-15 +

unc-13

P0: KR235 (Wildtype)

12 mM EMS

dpy-5

dpy-5

+

unc-15 +

unc-13+

dpy-5

dpy-5

+

unc-15 +

unc-13

+dpy-5

dpy-5

+ unc-13

+dpy-5

dpy-5 unc-15 +

dpy-5

dpy-5

+ unc-13

dpy-5

dpy-5 unc-15 +

sDp2

+ unc-13 + unc-13

+unc-15 +unc-15

(Dpy) (Wildtype)

(Unc-13)

(Unc-15)

(Dpy Unc-13)

(Dpy Unc-15)

*

*

*

*

*

*

dpy-5

dpy-5

+

unc-15 +

unc-13+

dpy-5

dpy-5

+

unc-15 +

unc-13

+dpy-5

dpy-5

+ unc-13

+dpy-5

dpy-5 unc-15 +

dpy-5

dpy-5

+ unc-13

dpy-5

dpy-5 unc-15 +

+ unc-13 + unc-13

+unc-15 +unc-15

(Let Dpy) (Wildtype)

(Unc-13)

(Unc-15)

(Let Dpy Unc-13)

(Let Dpy Unc-15)

*

*

*

*

*

*

F1 F2

*

*

*

*

*

*

Maintain