Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells

Zhang Li Jiangsu Institute of Nuclear Medicine

Wuxi, Jiangsu, China

Contact: zhangli@jsinm.org

10th AOTA CongressBALI, INDONESIA, 2012

Curcumin

An acitve polyphenol derived from the plant turmeric

low toxicity

anti-inflammatoryantioxidantantiangiogenic antitumour

Chemical structure of curcumin

Curcumin Thyroid cancer ?

Apoptosis and Autophagy

Apoptosis –type I programmed cell death

Autophagy- type II programmed cell death

- a self-degradative process

- cope with stresses

- required in apoptotic-deficient cells

Three types of autophgay:

- Microautophagy- Chaperone-mediated autophagy- Macroautopahgy

Part I

Crucumin suppresses BCPAP cells growth

A B

Effects of curcumin on cell viability

Effects of curcumin on cell morphology

PI/Hoechst 33342 staining

A B

Part II

Curcumin induces apoptosis in BCPAP cells

Effects of curcumin on pro-apoptotic and anti-apoptotic proteins

A

B

Effects of curcumin on loss of mitochondrial membrane potential (MMP)

Caspase-dependent apoptosis in

curcumin-treated cells

Part III

Curcumin induces autophagy in BCPAP cells

Induction of autophagy in curcumin-treated cells

A

B

The FASEB JournalJune 2003vol. 17 no. 9 1165-1167

RF

P-L

C3

Curcumin (mM)

0 50

C

Induction of autophagy in curcumin-treated cells

Effects of curcumin on the phosphorylation of AKT pathway

A

B

Effects of curcumin on the phosphorylation of MAP kinases

Curcumin selectively induces autopagy in tumor cells

A

B

Curcumin seletively induces cancer cell death

A B

Dynamic distribution of curcumin in BCPAP cells

Enlarged

6 h

12 h

24 h

Effects of curcumin on DNA conformation

A

B

Conclusions

Curcumin induces cytotoxicity of BCPAP cells.

Induction of  apoptosis  and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin .

Curcumin inhibited AKT pathway and activated mitogen-activated protein kinase (MAPK) signaling thereby inducing autophagy.

Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei

China Medical University

Guan Haixia

Nangjing University Sun Yang

AcknowledgementsJiangsu Institute of Nuclear Medicine Grants

National Natural Science Foundation of China (No. 30870727)

Natural Science Foundation of Jiangsu Province (No. BK2010156)

Science and Research Foundation of Health Bureau of Jiangsu Province (No. H2008031)