Post on 31-Dec-2015
description
Chromosome aberration Chromosome aberration analysis in peripheral analysis in peripheral
lymphocytes of Gulf and lymphocytes of Gulf and Balkans war veteransBalkans war veterans
H.Schröder, A.Heimers, R.Frentzel-Beyme, A.Schott and W.Hoffmann
Radiation Protection Dosimetry, 103:211-219 (2003)
Human T-lymphocyte metaphaseHuman T-lymphocyte metaphase
Karyotype 46, XY
Chromosome- type aberrations Chromosome- type aberrations distinguishable cytologically at mitosisdistinguishable cytologically at mitosis
Two normal chromosomesdicentric chromosome and acentric fragment
centric ring chromosome and acentric fragment
Aberrant metaphaseAberrant metaphase
Metaphase with two dicentric chromosomes and two acentric fragments
Exclusion criteriaExclusion criteria
•Previous radiotherapy and/or medical use of cytotoxic drugs
•Greater than average exposure to diagnostic medical radiation
•Heavy smokers (more than 20 Cigarettes a day over more than 10 years)
•Previous work in the nuclear industry or other badgemonitored occupation
2nd division metaphase with sister chromatid 2nd division metaphase with sister chromatid exchanges (SCE)exchanges (SCE)
Results of the chromosome aberration analysisResults of the chromosome aberration analysis
1 41 G 1001 4 0,0042 37 G 1002 3 0,0033 44 G 1001 7 0,0074 41 B 1003 2 0,0025 36 G 1001 3 0,0036 42 G 1003 2 0,0027 30 B 1002 2 0,0028 32 G 1000 4 0,0049 57 G 1005 1 0,001
10 38 G 1000 1 0,00111 49 G 1002 2 0,00212 34 G+B 1002 3 0,00313 46 G 999 3 0,00314 31 G 1001 1 0,00115 29 G 1001 1 0,00116 54 G 1001 3 0,00317 41 G 938 0 0,00018 40 G 1007 2 0,00219 32 G 1001 3 0,003
Total 18970 47 0,0025Control 45259 23 0,0005
mean frequency
per cellSubject Age
War of the Gulf, G
Balkans, B
Number of analysed
cells
Number of dicentrics
and centric rings
Result of the chromosome aberration Result of the chromosome aberration analysis after groupinganalysis after grouping
GroupNumber of cells
analysed
Number of dic +
cRMean 95%CI- 95%CI+
Gulf, subjects (N=16)
15963 40 0,0025 0,0018 0,0034
Balkans (N=2)
2005 4 0,0020 0,0005 0,0051
Gulf and Balkans (N=1)
1002 3 0,0030 0,0006 0,0088
Distribution of the observed dicentric chromosomes Distribution of the observed dicentric chromosomes and centric ring chromosomesand centric ring chromosomes
Subject u0 1 2
1 998 2 1 1,497 12,852 999 3 0 0,9983 995 5 1 1,280 6,764 1001 2 0 0,9995 998 3 0 0,9986 1001 2 0 0,9997 1000 2 0 0,9998 997 2 1 1,497 12,859 1004 1 0
10 999 1 011 1000 2 0 0,99912 999 3 0 0,99813 996 3 0 0,99814 1000 1 015 1000 1 016 998 3 0 0,99817 938 0 018 1005 2 0 0,99919 998 3 0 0,998
Total 15985 36 3 1,140 12,71Control 45259 23 0 1,000
Variance/ Mean
Distribution
Very rare findings
• Multicentric ring chromosome in a tetraploid metaphase, most probably consting of 4 chromosomes
• One metaphase with pulverized chromosomal material
Results, summaryResults, summary
• Method of chromosome aberration analysis is both sensitive and specific
• 5-fold elevation of dic and cR compared to the control (2.5 per 1000 M vs 0.5 per 1000 M)
• elevation is highly significant• significant elevation of dic and cR also in 9 of the
19 individual cases• distribution of dic and cR deviates from Poisson• very rare finding of a multi-cR in a tetraploid
metaphase and pvz chromosome material• biological half life of dic is 3.5 years, contradicts
to an acute radiation event
Results of the SCE-AssayResults of the SCE-Assay
1 50 209 0-9 4,18±0,312 50 246 1-10 4,92±0,293 50 223 0-10 4,46±0,364 35 135 1-9 3,86±0,375 47 139 0-9 2,96±0,286 50 246 0-11 4,92±0,358 50 219 0-10 4,38±0,319 50 198 0-9 3,96±0,30
Total 382 1615 0-11 4,23±0,12Control 486 2990 0-24 6,16±0,31
Number of cells
analysedSubject
Number of SCE
Range SCE per cell
SCE per cell mean±SEM