BioUML

0100100010011101

ISB0100100010011101

Fedor Kolpakov

Institute of Systems Biology(spin-off of DevelopmentOnTheEdge.com)

Laboratory of Bioinformatics,Design Technological Institute of Digital Techniques

Novosibirsk, Russia

Agenda

• Part 1: overview of BioUML workbench

• Cafe break

• Part 2: new concepts and possibilities(versions 0.8.0 – 0.8.3)

• Further development

• Questions and discussion

Part 1: overview of BioUML workbenchOverview• Main concepts• Meta model• Architecture overview• Diagram types• Database module

concepts• Full text search• Graph search• Simulation engine• BioUML server• BMOND/Biopath database

Live demonstration:•Installation of BioUML workbench•Creating and simulating simple model•SBML - Biomodels module•BioPAX import•BMOND database web interface•JavaScript shell

Part 2: new concepts and possibilities

Overview• Reconstruction as

solitaire game• Levels of biological

information• BioHub concept• Composite database

module• Composite diagram• Experiment concept• Graphic notation editor• Microarray data analysis

Live demonstration•Loading database modules from server•Text search•Graph search•Creating of composite database module•Creating of composite diagram•Experiment•Graphic notation editor•Microarray data analysis

Useful resourceshttp://www.biouml.org/demo

Flash movies that demonstrates how to work with BioUML workbench

http://www.biouml.org/user/help/index.html

http://www.biouml.org/download/0.7.8/manual.doc

Useguide, >200 pages

- HTML version

- MS Word document

http://bmond.biouml.org

Examples of pathway annotation:

BMOND – Biological Models aNd Diagrams database

Part 1

Overview of BioUML workbench

Main BioUML concepts and ideas• Visual modeling

o Meta model – problem domain neutral level of abstraction that describes system as compartmentalized graph

o Diagram type concept – formally defines graphical notation and provides its incorporation into BioUML workbench.

o Automated code generation for model simulation.• Database module concept - allows developer to incorporate

databases on biological pathways into BioUML workbench taking into account database peculiarities.

• Plug-in based architecture (Eclipse platform runtime from IBM company).

Biological databases

Data search and retrieving

Visual modeling

Automated code generation for model simulation of model behavior

Formal description of structure of biological system

MATLAB code Java code

Simulating using MATLAB.JMatLink allows to BioUML

workbench to start MATLAB and retrieve simulations results

Java simulation plug-in.Contains ODE solvers ported from odeToJava and methods

for hybrid models support.

… code

Meta model

][1 Akdt

][2][1 BkAkdt

][2 Bkdt

Corresponding mathematical model:

Example: system from two chemical reactions

A B-k1[A] k1[A]

-k2[B] K2[B]

R2100 0 0

k1 - reaction rate for R1k2 – reaction rate for R2

-k1[A] k1[A]

-k2[B] k2[B]

100 0 0

System structure is described as a graph

Mathematical modelof the system

Description of system components in the database

ID ACC .....//

ID R1A->B

ID BCC .....//

ID R2B->C

ID CCC .....//

A B-k1[A] k1[A]

-k2[B] k2[B]

R2100 0 0

Meta-model: example of formal description of system from two chemical reactions

Suggested approach can be applied for modeling biological systems using:– Systems of ordinary differential equations– Systems of algebra-differential equations– State and transition diagrams– Hybrid models– Boolean and logical networks– Petri nets– Markov chains– Stochastic models– Cellular automates– …

Some limitations– Spatial models– PDE– …

BioUML architecture

Plug-in based architecture

Plug-in- plugin.xml

- Java jar files

A plug-in is the smallest unit of BioUML workbench function that can be developed and delivered separately into BioUML workbench. A plug-in is described in an XML manifest file, called plugin.xml. The parsed contents of plug-in manifest files are made available programmatically through a plug-in registry API provided by Eclipse runtime.

- extension points are well-defined function points in the system where other plug-ins can contribute functionality.

- extension is a specific contribution to an extension point. Plug-ins can define their own extension points, so that other plug-ins can integrate tightly with them.

Plug-in - plugin.xml

- Java jar filesPlug-in

- plugin.xml- etc.

Eclipse platform runtime

Meta model

Executable model

Graph structure

Standard module

Database

Database adapter

Java objects

Gene Protein …

Diagram types- Semantic map- Pathway- Pathway simulation

Eclipse platform runtime Workbench UI

Diagram editor

Analysistools

Simulation tools

Other tools

Views, editors

Menus, toolbars, etc.

GeneNet module

KEGG/pathwaysmodule

TRANSPATHmodule

SBML module

Perspectives

Diagram view part

Diagram editor part

DiagramType-semantic controller-diagram view builder-diagram filter

ModuleType-diagram types-data categories-query engine

Query engine

Formal description and modeling of biological systems require coordinated efforts of different group of researchers:

• programmers - they should provide computer tools for this task.

• problem domain experts - they should specify what and how should be described.

• experimenters and annotators - they should describe corresponding data following to these rules.

• mathematicians - they should provide methods for models analysis and simulations.

BioUML architecture separates these tasks so they can be effectively solved by corresponding group of researchers and provides simple contract how these groups and corresponding software parts should communicate.

Diagram types

Diagram type conceptDiagram type defines:

· types of biological components and their interactions that can be shown on the diagram;

· diagram view builder - it is used to generate view for each diagram element taking into account problem domain peculiarities;

· semantic controller - provides semantic integrity of the diagram during its editing;

· filters – hide or highlight diagram elements according to some selection criteria.

Reconstruction and formal description of biological systems using different diagram types

1. Semantic network

2. Pathway diagram(semantic network + gene network or metabolic pathway)

3. Metabolic pathway 4. Gene network

5. Pathway simulation(mathematical model)

Formality,details Semi-structured

Structured data(reactions and its components)

Kinetic data(kinetic laws, constants,

initial values

Graphic notation

Stimulus activating NF-kappaB(semantic network, ontology)

NF-kappaB family (semantic network, ontology)

Function of human DNA methyltransferases (pathway diagram)

The biosynthesis of catecholamines(metabolic pathway)

Cell cycle model of mammalian G1/S transition control with E2F feedback loops

(pathway simulation diagram)

DGR0356 “NF-kB model” (Hoffmann et al., 2002)

NF-kB dynamics in nucleus and cytoplasm before and after TNF-alpha stimulation (Hoffmann et al., 2002)

Regulation of caspase-3 activation and degradation (Stucki and Simon, 2005 )

Database module conceptThe database module concept allows to

developer define new diagram types and incorporate other databases on biological pathways into BioUML framework.

The database module defines mapping of database content into diagram elements and diagram types that can be used with the database.

Module also provides query engine that can be used by BioUML workbench to find interactiong components of the system.

BioUML database modulesBioUML standard module

Databases• EBI databases: Ensembl, UniProt, ChEBI, GeneOntology• Biopath/BMOND (http://biopath.biouml.org)• KEGG/Ligand (http://www.kegg.com)• TRANSPATH (http://www.biobase.de) • GeneNet (http://wwwmgs.bionet.nsc.ru)

Formats• SBML – Systems Biology Markup Language, level 1, 2

(http:// www.sbml.org)• CellML – Cell Markup Language (http://www.cellml.org)• BioPax – Biological Pathways Exchange (http://www.biopax.org)• PSI-MI• OBO• GXL - Graph eXchange Language (http://www.gupro.de/GXL)

KEGG pathway

CellML model

SBML model

Full text search

User interface for full text search: 1) pop-up menu; 2) menu buttons for selected entity; 3) full text search pane.

Full text search (uses Lucene engine)

Graph search

Graph search engine

Simulation engine

Biological databases

Data search and retrieving

Visual modeling

Automated code generation for model simulation of model behavior

Formal description of structure of biological system

MATLAB code Java code

Simulating using MATLAB.JMatLink allows to BioUML

workbench to start MATLAB and retrieve simulations results

Java simulation plug-in.Contains ODE solvers ported from odeToJava and methods

for hybrid models support.

… code

%script for 'CellCycle_1991Gol' model simulation

%constants declarationglobal Reaction1_vi Reaction2_kd Reaction4_K1 Reaction4_Kc Reaction4_VM1 Reaction5_K3

Reaction5_VM3 Reaction6_K2 Reaction6_V2 Reaction7_K4 Reaction7_V4Reaction1_vi = 0.023Reaction2_kd = 0.00333Reaction4_K1 = 0.1Reaction4_Kc = 0.3Reaction4_VM1 = 0.5Reaction5_K3 = 0.1Reaction5_VM3 = 0.2Reaction6_K2 = 0.1Reaction6_V2 = 0.167Reaction7_K4 = 0.1Reaction7_V4 = 0.1

%Model rate variables and their initial valuesy = []y(1) = 0.0 % y(1) - $cytoplasm.Cy(2) = 0.0 % y(2) - $cytoplasm.EmptySety(3) = 0.0 % y(3) - $cytoplasm.My(4) = 0.0 % y(4) - $cytoplasm.X

%numeric equation solving[t,y] = ode23('CellCycle_1991Gol_dy',[0 100],y)

%plot the solver outputplot(t,y(:,1),'-',t,y(:,2),'-',t,y(:,3),'-',t,y(:,4),'-')title ('Solving Goldbeter problem')ylabel ('y(t)')xlabel ('x(t)')legend('$cytoplasm.C','$cytoplasm.EmptySet','$cytoplasm.M','$cytoplasm.X');

Function to calculate dy/dt for the model

function dy = CellCycle_1991Gol_dy(t, y)% Calculates dy/dt for 'CellCycle_1991Gol' model.

%constants declarationglobal Reaction1_vi Reaction2_kd Reaction4_K1 Reaction4_Kc Reaction4_VM1 Reaction5_K3

Reaction5_VM3 Reaction6_K2 Reaction6_V2 Reaction7_K4 Reaction7_V4

% write rules to calculate some eqution parametersrateOfReaction1 = Reaction1_vi;rateOfReaction4 = ((1 - y(3))*Reaction4_VM1*y(1))/((1 + Reaction4_K1 -

y(3))*(Reaction4_Kc + y(1)));rateOfReaction5 = (Reaction5_VM3*(1 - y(4))*y(3))/(1 + Reaction5_K3 - y(4));rateOfReaction6 = (y(3)*Reaction6_V2)/(Reaction6_K2 + y(3));rateOfReaction7 = (Reaction7_V4*y(4))/(Reaction7_K4 + y(4));rateOfReaction2 = y(1)*Reaction2_kd;

% calculates dy/dt for 'CellCycle-1991Gol.xml' modeldy = [ + rateOfReaction1 - rateOfReaction2 - rateOfReaction1 - rateOfReaction4 - rateOfReaction5 + rateOfReaction6 +

rateOfReaction7 + rateOfReaction2 + rateOfReaction4 - rateOfReaction6 + rateOfReaction5 - rateOfReaction7]

Results of SBML semantic tests

BioModels – comparison BioUML simulation

results with other simulators

http://www.biouml.org/_biomodels/

Simulators comparison criteriaPassed – CSV file was generated by simulator

interval criteria no difference - 0.999 * min < x < 1.001 * max or x < ZERO and max < ZERO small difference – 0.5 * min < x < 1.5 * max significant difference - otherwise

median criteria no difference - abs((x – median)/median) < 0.01 or x < ZERO and median < ZERO small difference - abs((x – median)/median) < 0.5 significant difference – otherwise

x – variable value provided by compared simulatormin, max, median – calculated from values provided by other simulators with

which the specified simulator is being compared.

Implementation note: if result file was not generated by BioUML, then other simulators can be compared one to each other.

BioUML Enterprise Edition:BioUML server

BioUML workbench

Servlet container: Tomcat

BioUML EE architecture

MySQL database

Web browser

BeanExplorerEnterprise Edition

Client side:

Server side:

Database module

BioUML servlet

JDBC DB module

Lucene full textsearch engine

BMOND Biological MOdels aNd Diagrams

database

(former name – Biopath)

BioUML workbench

Servlet container: Tomcat

BMOND system architecture

BiopathMySQL database

Web browser

BeanExplorerEnterprise Edition

Client side:

Server side:

Biopath module

Figure 4. G1/S entry model (Kel et al., 2000) described using BioUML technology.

BMOND web interfacelive demonstration

http://bmond.biouml.org

- Interface overview- View diagrams- View diagram components- List of diagram components- Categories (classification)- Filter- Dynamic columns- Web forms for components editing

Part 2

New concepts and possibilities

Part 2: new concepts and possibilities

Overview• Reconstruction as

solitaire game• Levels of biological

information• BioHub concept• Composite database

module• Composite diagram• Experiment concept• Graphic notation editor• Microarray data analysis

Live demonstration•Loading database modules from server•Text search•Graph search•Creating of composite database module•Creating of composite diagram•Experiment•Graphic notation editor•Microarray data analysis

Metaphor: biological systems reconstruction as solitaire (patience) game

Desk – BioUML editor

Solitaire – biological pathway

Cards – biological objects(genes, proteins, lipids, etc.)

Pack of cards – different biological databases

UniProtEnsembl ChEBI GOLevel 1: Catalogs

Level 2: Pathways,models

GeneModels

Biological objects

Levels of biological information

refers

Level 3: Problem specific

Cyclonet- leads- actions- targets

refers refers

LipidNetclassifications:- lipids- genes

refers

UbiProtclassifications:E1, E2, E3, …

Main idea for data integration and pathway reconstruction: - escape information duplication- classify components of biological pathways by levels- each next level should refer but do not duplicate information from previous levels- use free EBI databases whenever it is possible.

refers

Add-on technologyThis approach should help us to solve difficulties with usage of external catalogs when external catalog does not contain needed entity (for example gene or substance) or when we would like to add some information to existing entity description. Example for BMOND2, gene: special table allow us to add new entity to BMOND2 if such entity missing in corresponding external catalog.

Gene catalogEnsembl

Geneadd-on table

Synonyms

Description

DB references

Literature references

Classification

SQL query

BioUML

BeanExplorerWeb interface

Java object

Lucene

Document

BioHub

BioHub concept• BioHUB – an approach link information from different databases.

Main usage:– binding microarray (omics) data to pathway diagrams– graph search– DBReferences editor– microarray (omics) data analysis

• Follows to MIRIAM standard:– References to database objects– Relationships between biological objects

• Simple Java API

BioHub structure Entities- DB_ID- version- ID- AC- species- description- key words

Relations- DB_ID_1- DB_version_1- ID_1- DB_ID_2- DB_version_2- ID_2- relation- evidence- comment

Databases- DB_ID- name- description- URL- url_patern_ID- url_patern_AC

RelationTypes- relation- description- backwardRelation- comment

RelationInfo- DB_ID_1- DB_ID_2- relation- comment

GeneModels

Biological objects

Linking with experimental data and results of analysis

refers

Experimental data,results of analysis

BioHUB

OMICS data

Results of analysis

MSigDB GeneAtlas,NCI60

refers refers

refers

GeneModels

Biological objects

Linking with external databases

refers

Experimental data,results of analysis

BioHUB

OMICS data

Results of analysis

MSigDB GeneAtlas,NCI60

External databases:- KEGG- LipidMap, LipidBank- Reactome, …

refers refers

refers

Coloring diagram according to microarray data.Each bar corresponds to one value from corresponding microarray series.

Coloring diagram according to omics data

BioHub usage: graph search engine

Composite database module

Flash movie: XML_module.exe

Composite database module is defined formally as XML document. It allows:• specify dependencies from other database modules• specify data types that can be used from external database modules• describe dynamic properties for add-on technology• specify what dynamic properties can be added to data types from external modules. This information will be stored in local module and merged dynamically with information from external modules. By this way user can add information to external catalogs like Ensembl, UniPropt, etc.• specify data types used by local module• specify diagram types used by local module• specify QueryEngine

Composite database module

<!ELEMENT dbModule (jdbcConnection, properties?, dependencies?, types?)>

<!ATTLIST dbModule > name CDATA #REQUIRED title CDATA #REQUIRED description PCDATA version CDATA "0.8.0" type CDATA text|SQL databaseType CDATA databaseVersion CDATA databaseName CDATA >

<!ELEMENT jdbcConnection><!ATTLIST jdbcConnection> name CDATA #REQUIRED jdbcDriverClass CDATA #REQUIRED jdbcURL CDATA #REQUIRED jdbcUser CDATA jdbcPassword CDATA>

<!ELEMENT properties (property*)><!ELEMENT property (tags?)><!ATTLIST property name CDATA #REQUIRED type CDATA #REQUIRED short-description CDATA #IMPLIED value CDATA ><!ELEMENT tags (tag+)><!ELEMENT tag><!ATTLIST tag name CDATA #REQUIRED value CDATA #IMPLIED ><!ELEMENT propertyRef><!ATTLIST propertyRef name CDATA #REQUIRED value CDATA >

<!ELEMENT dependencies (dbModule*, graphicNotation*)><!ELEMENT dbModule (externalType+)><!ATTLIST dbModule> name CDATA #REQUIRED ><!ELEMENT externalType (propertyRef*)><!ATTLIST externalType name CDATA #REQUIRED readOnly CDATA true|false ><!ELEMENT graphicNotation><!ATTLIST graphicNotation> name CDATA #REQUIRED type CDATA Java|XML class CDATA path CDATA>

<!ELEMENT types (internalType*)><!ELEMENT internalType (querySystem, propertyRef*)><!ATTLIST internalType> section CDATA #REQUIRED name CDATA #REQUIRED class CDATA #REQUIRED transformer CDATA #REQUIRED ><!ELEMENT querySystem (index*)><!ATTLIST querySystem> class CDATA #REQUIRED luceneIndexes CDATA><!ELEMENT index><!ATTLIST index> class CDATA #REQUIRED table CDATA>

Editor for composite database module

Current status:Implemented:• Database modules (initial version):

Ensembl, UniProt, ChEBI, GO,IntAct, Reactome, BioModels

• Composite module (external referencies)– Defined as XML– Composite module editor

• Selecting and loading modules from server

In process:• BioHUB• Protein state concept• Add-on technology• BMOND2 – redesigned version of BMOND.

From huge theory to practical output

Automated language translation

Practical output• electronic dictionaries• spell checkers

Biological data

integrations

Practical output• catalogs (Ensembl, UniProt, CheBI)• controlled vocabularies, ontologies• hubs

Model composition

Composite diagram: main conceptsblock (EModel)dx/dt = f1dy/dt = f2z = f3

block 2 (EModel)dx/dt = f5dy/dt = f6 + z k+z+f4 = 0

subdiagram (EModel)

Rs1 s2

block 3 (EModel)dx/dt = f5 dy/dt = f6 + block2.k k+z+f4 = 0

Indirect linkx

xforbidden

Rs2 s3

s4direct participation of subdiagram elementin a reaction

Block types:1) block – only mathematical equations. Used mainly for physiological models;2) subdiagram – other diagram

Connection types:1) directed – input output. Transformation function can be used;2) undirected – contact. Indicates that 2 nodes in mode is the same entity.

Semantic constraints:There are semantic constraints, for example: block can have only one input for each variable. Two inputs are forbidden for the same variable.

Flat model:Before Matlab or Java code generation composite model is transformed into flat model and usual genertions routines are used.

Experiment

To make a virtual experiment it is frequently needed to modify initial model.

Typical modifications (changes) are:• changing of initial values• changing of model parameters to imitate different conditions or mutations• deleting of some model elements to imitate knock-out mutations• adding events to imitate external influences on the model

To skip model duplications for each virtual experiment we introduce “changes” concept.

Graphic notation

formal definition as XML document

http://www.biouml.org/sbgn.shtmlFlash movie: Graphic_Notations_Editor.exe

Graphic notation versus graph layout

• allows edit diagram• allows to create new diagram• different graphic notations can be applied

to the same SBML model• allows formally define SBGN and use it in

SBML models• allows to reuse graphic notation by many

Graphic notation can be defined formally as XML document• properties – formal definition of properties that can be used as properties of nodes and

edges (for example, title, multimer, etc.). Definition of property includes:– name– type– short description– controlled vocabulary (optional)

• node types – definition of node includes:– name– icon – properties– view function (JavaScript)– short description

• edge types – definition of edge includes:– name– icon – properties– view function (JavaScript)– short description

• semantic controller – defines rules for semantic control of diagram integrity. For this purpose it defines following functions:

– canAccept (JavaScript)– isResizable (JavaScript)– move (JavaScript)

• Examples – a set of diagrams that can be used as test cases, legend and examples for the graphic notation. DML - Diagram Markup Language – is used for this purpose.

SBML …

Diagram

Model API

BioPAX Layoutinformation

Graphic notation

Layout APINotation API

Rendering engine JavaScript functions:

- build node/edge view

- semantic control

Initial data

JavaScriptAPI for

data access

Rendering APIJavaScript API for creating primitives

similar with SBML layout extension

Basic software architecture for rendering of biological models according to specified graphic notation and layout information

Formal definition of graphic notation as XML document and integration with SBML format

Graphic notation components

Defined as SBML

Object types XML <annotations> Object properties XML <annotations> User defined properties XML <annotations> Rules for visualization JavaScript Rules for semantic control JavaScript Test cases XML model, module

Graphic notation editormain concepts

• graphic notation is defined formally as XML document• graphic notation editor provides user friendly interface for

XML document editing• SBGN graphic notation (prototype) is implemented• BioUML workbench allows to create and edit diagrams

using graphic notation defined as XML document• May be graphic editor will be useful for SBGN

community for:– improving SBGN specification– for testing SBGN specification by creating different diagrams

Details: http://www.biouml.org/sbgn.shtml

BioUML workbech

Select ‘Data’ tab to see the tab with a listwith available graphic notations

Click right mouse button onselected graphic notation to open it Graphic Notation Editor

Graphic Notation Editor

Main sections of formal definitionof graphic notation

List of specific propertiesthat are used by graphic notation

Properties editor

User can click right mouse button on Properties node to create new property

Nodes – contains list of all node typesused by graphic notation

For each node type user can define:- name- properties- icon- view function (JavaScript)

By clicking right mouse button on “Nodes”user can create new node type

By the same way user can define edge type:- name- properties- icon- view function (JavaScript)

“Examples” nodecontains a set of diagrams that demonstrates usage of graphic notation.

User can create and edit such diagram.

When user selects some element on the diagram he can edit:- object properties- JavaScript that builds a view for selected diagram element

“Semantic controller” nodecontains list of JavaScript functions that provide semantic constraints and semantic integrity of the diagram.

Graphic notation defined as XML document can be used by BioUML workbench to create corresponding diagram.

Graphic Notation Editor

SBGN examplescreated in BioUML

Microarray plug-in(alpha version)

Microarray plug-in- Import microarray data in tab delimited format- Show data as a table- Filter data by different criteria- Microarray data analysis

- Revealing up/down regulated genes- Meta-analyses

- Binding with diagram nodes by ID- Coloring diagrams- JavaScript functions

- Data manipulation (filter, join, intersect, trim, etc.)- Statistical analysis

Microarray plug-inCurrent work:- Powerful user interface for coloring diagrams- Support of other formats for microarray data and results

of analyses- Sophisticated binding algorithm using different database

references and ID (gene hub)

Further work:- Server module that will provide access to ArrayExpress

BioUML workbench.

Data tab contains section “Microarray”.User can import microarray data in tab delimited format into this section.

Possibility to filter probe sets:- by column values - selecting only those probe sets that can be linked to the specified diagram

Microarray analysis

Coloring diagram according to microarray data.Each bar corresponds to one value from corresponding microarray series.

Coloring diagram according to omics data

Further development:

Protein state

BioUML workbench: further development• Protein states• Complexes

• Improving team work on annotation– Login, single sign on– Editing history (what data were modified, whom and when)– Passing of changes from server to client

• Sequence analysis and visualization

• Agent based modeling

Protein state

Modification• The functions of macromolecular entities (mainly proteins)

are often determined not only by their primary sequences, but by chemical modifications they have undergone.

• In BMOND2 unmodified and modified forms of a protein refer to the same entity in UniProt database

• List of possible modifications is extracted from UniProt Feature Table

• BMOND2 modifications table– allows to describe modifications that are not described in UniProt.

These modifications are automatically added to the protein, referred from BMOND2.

• Modification type – control vocabulary that describes possible modification types (for example, phosphorylation, acetylation, ubiqutination)

• To take into account protein modifications State concept is used.

UniProt Feature Table•FT CHAIN 1 561 Cytosolic purine 5'-nucleotidase.•FT /FTId=PRO_0000064389.•FT REGION 202 210 Substrate binding (Potential).•FT COMPBIAS 549 561 Asp/Glu-rich (acidic).•FT ACT_SITE 52 52 Nucleophile.•FT ACT_SITE 54 54 Proton donor.•FT METAL 52 52 Magnesium.•FT METAL 54 54 Magnesium (via carbonyl oxygen).•FT METAL 351 351 Magnesium.•FT BINDING 127 127 Allosteric activator 1.•FT BINDING 154 154 Allosteric activator 2.•FT BINDING 354 354 Allosteric activator 2.•FT BINDING 436 436 Allosteric activator 1; via carbonyl•FT oxygen.•FT BINDING 453 453 Allosteric activator 2.•FT MOD_RES 527 527 Phosphoserine (By similarity).•FT VARIANT 3 3 T -> A (in dbSNP:rs10883841).•FT /FTId=VAR_024244.•FT VARIANT 136 136 Q -> R (in dbSNP:rs12262171).•FT /FTId=VAR_030242.

Modification

• position

• amynoacid

• modification type (controlled vocabulary)

• evidenceexperimental, by similarity, predicted

• comment

• Publication reference

State concept• State – describes states of all amino acids available for

modifications• possible values:

– ? – unknown, not specified– * – any– - – unmodified– p – phoshporylated– ac – acetylated– … – from controlled vocabulary

• Protein states are described in BMOND2 states table• Reaction – user should specify protein state• Diagram – user should specify protein state

State table

• module (database)

• id

• state – short name (like TRANSPATH)

• position

• modification

Mapping: BMOND2 -- SBGNmodification – state variablestate – state of macromolecule

Complex concept

• A complex is s a biochemical entity composed of other biochemical entities, whether macromolecules, small molecules, multimers, or themselves complexes.

• Complex is specified as a set of units• Complex modifications

– all possible modifications of its units (some of them can not occur due to physical interactions between units – how we can take it into account)

• Complex state– var.1 – list of modifications for its subunits– var. 2 – list of states for its units

Complex tables• Complex

– ID– title (short name)– complete name– species– synonyms– comment

• References:– States– Synonyms– Structure– DBReferences– Publications

• Complex Units– complexDB– complexID– unitDB– unitID– multimer

Reaction• Reaction components

– component identification• DB• id• [state]• [compartment]

• Reaction– [compartment]

• Reaction dialog– specie state– specie compartment– reaction compartment

• Tables– Reaction

• compartment– Reaction

components• state• compartment

Diagrams

• Macromolecule state– “New diagram element” dialog

• Graphic notation– BioUML

• states – right label, one modification• complexes

– SBGN skin

AcknowledgementsPart of this work was partially supported by following grants: • European Committee grant №037590 “Net2Drug”• Siberian Branch of Russian Academy of Sciences

(interdisciplinary projects № 46)• Volkswagen-Stiftung (I/75941), • INTAS Nr. 03-51-5218• RFBR Nr. 04-04-49826-а

Author is grateful to for useful comments, discussions and technical support

Alexander Kel Sergey Zhatchenko

Software developers Annotators Nikita Tolstyh Mikhail Puzanov Ruslan Sharipov Sergey Lapukhov Ilya Kiselev Ivan YevshinAlexander Magdysyuk Denis Ryumin Elena CheremushkinaVlad Zhvaleev Alexandr Koshukov Ekaterina Kalashnikova Vasiliy Hudyakov Igor Tyazhev Sergey Graschenko Oleg Onegov

BioUML

Documents

Transcript of BioUML

BioUML SOFTWARE FRAMEWORK FOR SYSTEMS BIOLOGY Overview 2004 - 2010 ITC Software All rights reserved.

BioUML Fedor Kolpakov Institute of Systems Biology (spin-off of DevelopmentOnTheEdge.com) Laboratory of Bioinformatics, Design Technological Institute.

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BioUML – open source extensible workbench for …sbml.org/images/f/fa/20041014-kolpakov-biouml.pdf2004/10/14 · layer for comprehensive formal description of wide range of biological

BioUML – open source integrated platform for collaborative and reproducible research in systems biology Fedor Kolpakov, Institute of Systems.

BioUML integrated platform for building virtual cell and virtual physiological human

The BioUML platform QUICK START GUIDE · logging in to the platform, you can change your password and edit your personal information. To get information about your account, click

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BioUML () Software framework for systems biology Overview Biosoft.Ru, Novosibirsk, Russia. Laboratory of Bioinformatics, Digital Design.

Building virtual cell using BioUML platform Ilya Kiselev (on behalf BioUML team) Institute of Systems Biology, Ltd. Novosibirsk, Russia Biosoft.Ru September.

Building virtual cell using BioUML platform Fedor Kolpakov Biosoft.Ru, Ltd. Institute of Systems Biology, Ltd. Novosibirsk, Russia Biosoft.Ru July 6th.

1 BioUML - extensible workbench for systems biology Laboratory of Bioinformatics, Novosibirsk, Russia. 2004 -2010 ITC Software All.

NGS data analyses with BioUML Fedor Kolpakov Biosoft.Ru, Ltd. Institute of Systems Biology, Ltd. Novosibirsk, Russia.

BioUML SOFTWARE FRAMEWORK FOR SYSTEMS BIOLOGY Overview 2004 - 2011 ITC Software All rights reserved.