Post on 23-Mar-2019
Bionote TB Feron ELISA,
1st screening tool of bovine TB
Manual of Diagnostic Tests and
Vaccines for Terrestrial Animals
Updated: 23.07.2004
OIE approved test method for bovine TB
Bovine Tuberculosis & Immunity
BTB Ab ELISA
TB Feron ELISA
PPD
TB Feron MKT point 1. Bionote detection system of all stage of
B.TB infection
TB Feron ELISA
B. TB Ab rapid test
(IgG/M)
Detection of all stage
Early stage Late stage
Recommended BTB Diagnosis Flow
TB Feron ELISA
Negative
BTB Rapid
Positive
Infected $6.25
Negative
Free
Positive
Infected $4
Enduser : $4/sample
Enduser : $2.5/sample
1st screening
2nd confirmatory
with TB feron ELISA as 1st screening tool instead of PPD
Detectable of all stage of B.TB
infection
Comparison Table Bionote TB Feron ELISA + BTB Ab RDT
vs PPD and Bovigam ELISA
TB feron ELISA and
BTB Ab RDT screening
PPD+Bovigam ELISA
screening
Detectability All stages of BTB
infection period
-Early stage of BTB
infection period.
-May miss the late stage
of infection
Time required a half day 3 days. 2 times visit
Where tested Laboratory Field
Reagent Cost $6.00/sample
BTB RDT $2
TB Feron $4
$12/sample
PPD : $1
Bovigam: $11
Labor cost 1 day cost (i.e. $50) 2day cost(i.e $100)
Mutiple testing Capable for multiple test
with B.brucella, FMD
ELISA
Impossible
Cost Comparison of 1st screening test Bionote TB Feron ELISA + BTB Ab RDT vs PPD and Bovigam ELISA
Cost of 10,000 cattle testing
TB feron ELISA PPD
Reagent cost $4 x 10,000 = $40,000 $1x 10,000=$10,000
Time required for
blood collection and
PPD injection
Cost
Blood collection :
50 cattle/person/day
Total 200 days
200 days x $50=$10,000
PPD injection
25 cattle/person/day
Total 400 days
400 days x $50=$20,000
Time required testing
performed
4 hours with result check 70 hours without result check
Revisit farm for result
check
No required.
No cost
Revisit required.
Cost incurrence
$30 x 250 days =$7,500
Time required result
check
No required 40 cattle/person/day= 250 days
Cost : 250 x $50 =$12,500
Total cost $50,000 $50,000
Cost Comparison for 2nd confirmatory testing Bionote TB Feron ELISA + BTB Ab RDT vs PPD and Bovigam ELISA
TB feron ELISA PPD
If positive result is happened 10%
Final positive confirmation, No required for further test
TB feron ELISA or other confirmatory test required
Time & cost required for blood collection for 2nd testing
No require. Already collected. No cost rise $0
Revisit Farm and collect the sample. -Blood collection : 20 days x $50=$1,000 -Reagent cost $4 x 1000 =$4,000 -Time cost : 20 days x $50 =$1000 Total = $6,000
If 90% negative result is happened
Further test(BTB Ab rapid/ELISA) for checking late stage of infection
Further test(BTB Ab rapid/ELISA) for checking late stage of infection
Cost for 2nd testing Blood already collected. Reagent cost = $2 x 9,000 =$18,000 Time cost: 3 days x $ 50 =$150 Total = $18,150
Revisit Farm and collect the sample. -Blood collection : 180days x $50=$9,000 -Reagent cost $2 x 9000 =$18,000 -Time cost : 183 days x $50 =$9,150 Total = $36,150
Total cost $0~ $18,150 $6,000 ~ $72,500
Cost of 10,000 cattle testing
TB Feron ELISA PPD tuberculin Bovigam
Sensitivity High Low High
Specificity High Low High
Result interpretation
Clear By ELISA reader
Unclear By eyes
Clear By ELISA reader
Retesting for suspected case
Easy and quick Difficult and late 2 months waiting.
Easy and quick
Total cost US$5.00/sample US$5.00/sample US$15.00/sample
Technology High & New Tech OIE approved
Low & Old Tech OIE approved
High & New Tech OIE approved
Base Laboratory Field Laboratory
Stimulation PPD Provided in the kit Nota applicable Not Provided Price: $0.60/sample
What are benefits of BIONOTE TB feron ELISA?
One
blood
collection
FMD (N)SP
Ab ELISA
TB Feron
ELISA
Brucella Ab
ELISA
TB Feron ELISA MKT Point 3 : All in one
BTB Diagnosis Flow 2 for Brazil : Cheaper than flow 1
AniGen B.TB Ab rapid test
Negative
TB Feron ELISA
Positive
Infected
Negative
Free
Positive
Infected Enduser : $4/sample
Enduser : $2.5/sample 1st screening
2nd confirmatory
Thank you for your attention