Biomoles RedTrack & GreenTrack Biomoles RedTrack & GreenTrack Gels ComparisonsGels Comparisons

Biomoles Inc.Tel: 206-877-0969biomole@biomoles.comwww.biomoles.com

Jan 2013Jan 2013

Key Specification Features of The

RedTrack & GreenTrack Precast Gels

Biomoles’ new generation RedTrack & GreenTrack Precast Gels which are away from ethidium bromide, not only address health and safety concerns, but provide superior performance & competitive pricing

Non-hazardous, non-toxic, safe to both researchers and environmental health. Gels can be disposed in garbage cans even after dried up.

High sensitive, sharp & clear DNA bands, remarkable clean background which enables DNA & RNA quality separation, as well as mini-fragments, tiny amount analysis (as low as 0.5ug).

Separate and visualize impurity contaminants from DNA & RNA.

Long term (2 days under RT, and 10mon at 4oC) fresh DNA bands gel preservation which are not only great convenience for delayed gel imaging after electrophoresis, but enables long-term DNA gel excise, mobile observation and even overtime presentation.

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RedTrack & GreenTrack Gels Compared with the ethidium bromide gel:

Superior sensitivity

Better performance

Easy-to-handle

Very clean background

Able to detect smallest size and tiny amounts of DNA with high resolution

Safe, non-toxic to replace traditional mutagen

Competitive Pricing

Competitive Advantage in Precast Gels

RedTrack/GreenTrack EtBr50bp DNA ladder, 4.5ng per lane

RedTrack GreenTrack EtBr10bp – 4kb, 5ng per lane

Innovative Tools and Diagnostics----------------------------------

Precast Agarose Gel

Sensitivity

GreenTrack & RedTrack

50bp DNA ladder, 4.5ng per lane

10bp – 4kb, 5ng per lane

RedTrack

Innovative Tools and Diagnostics----------------------------------

1.0% RedTrack Precast Gel

RNA as low as 0.5ng per wellUnder insufficient Red/OrangeLoading Dye

1st RNA Report from A Researcher

Note: the visibility of RNA bands could be improved by loading the recommended concentration of Red/Orange Loading dye

Red/& Green Track Precast Gels preserve DNA samples for more than

10 Months vs the current 15 minuntes --- A Historical Solution!

RedTrack Gel Fresh Image taken in Aug 16, 2007.Plasmid DNA Isolated from Biomolès Kit

RedTrack Gel Image 10 month Later, taken at May 8th, 2008. Plasmid DNA Isolated from Biomolès Kit

Extending DNA Gel-Life

Fresh image

10 months later

This feature is also a great advantage for delayed DNA gel imaging after electrophoresis, DNA gel excise, and even overtime presentation or mobile observations of the DNA bands

Innovative Tools and Diagnostics----------------------------------

Background and DNA Band Comparison Between the Ethidium Bromide Based Gels & The RedTrack Precast Gels

Ethidium Bromide Gel RedTrack Agarose Gel--- 20bp DNA with or without showing the bands

Innovative Tools and Diagnostics----------------------------------

DNA bands can be excised directly from the gel without hinder block as other precast gels may have

How To Use

Biomoles Precast Gels A. Open The Gel Pouch Using Scissors

How To Use

Biomoles Precast Gels B. Pill Off The Seal Cover, Pour Out Completely The Residual Buffer

How To Use

Biomoles Precast Gels C. Remove The Up & Down Sides of the Tray (with gel in) with

Ladder Banks By Flexing Them Down & Away One Side at a time (indication images)

How To Use

Biomoles Precast Gels D. Measure the internal height of your gel box to make the 14well

Tray Holder fit to it. Insert the side of the tray into the tray holder clip, placing the gel tray at the bottom of the gel box, hang

another end of the tray holder over the gel box wall.

How To Use

Biomoles Precast Gels E. If you have a

sample gel and do not have the gel tray holder, simply place The Tray at The Bottom of the Gel Box With Left Side Flush With The Side Wall of The gel box. Use peppit tip to stabilize the gel trays.

How To Use

Biomoles Precast Gels F. Add Buffer

(1XTAE or TBE) into the gel box. Load DNA or RNA samples. Cover the gel box, run Electrophoresis

How To Use

Biomoles Precast Gels F. Biomoles 8-

well & 16-well of Precast Gels Fit The Standard 7cm Width Of Gel Box As The Bio-Rad’s Mini-Sub Cell GT

Safe Disposal

Biomoles Precast Gels

1. You can dispose the precast gel after using into the regular garbage can let it dry out, it will be totally safe and non-hazardous.

2. Suggestion: please advise those still using ethidium bromide based gels to eliminate the mutagenic toxicity to human health.

3. Thanks for your support.

Contact: biomole@biomoles.com, or 206-877-0969

Biomoles DNA Purification Kits --- DNA PurityComparison

- Isolated genome DNA from tissue & buffy coat samples

- Compared with Q & M kits- DNA purity 15-20% greater- DNA yields 40% higher greater- Transfection grade Plasmid DNA- Use for any downstream applications

without further process

Thank You