Post on 12-Jan-2016
description
Benefits of scientific cooperation among European countries in medical research
Daniela Jezova
Institute of Experimental EndocrinologyInstitute of Experimental EndocrinologySlovak Academy of SciencesSlovak Academy of Sciences
BratislavaBratislava
Title of the Project
„Reduction of Negative Impact of Environmental Factors on Human Health“
Co-ordinator: Assoc.Prof. Daniela Ježová
Project of the Centre of Excellence supported by European Union
Institute of Experimental EndocrinologySlovak Academy of SciencesBratislava, Slovakia
Coordinator: Assoc. Prof. Daniela Ježová
Main „mission” of this Centre
To provide experimental and clinical data leading to specific recommendations aimed at reducing the negative impact of environmental factors on human health
STRESSNUTS
obtaining high quality scientific data with relevance to clinical research
significant improvement of the training process of young researches both from EU and Slovakia
active and broad cooperation among European scientists
Main positive achievements
obtaining high quality scientific data with relevance to clinical research
significant improvement of the training process of young researches both from EU and Slovakia
active and broad cooperation among European scientists
Main positive achievements
Most important scientific result (one selected from many)
Evidence has been obtained to support
further clinical research on the following
substances to modulate hormone release
and treat the negative impact of demanding
stressful situations, anxiety and depression:
mood stabilizers
extract of Ginkgo biloba
pentoxifylline
antidepressants
selected amino acids
Salivary cortisol (ng/ml)
-0.5
0
0.5
1
1.5
0 15 30 45min
chan
ges
fro
m b
asal
val
ues
stress
time: p<0.001treatment: p<0.05
placebo
lamotrigine
STRESSNUTS
Public speech
Prolonged anticipationProlonged speechEmotional topicsVideo recording of speechStanding during speechUse of microphoneBright lightOpen space
0
10
20
30
40
50
60
0 15 30 45 60 90
placebo
amino acids
time: p=0.023treatment: p=0.004
stress
ACTH(pg/ml)
0
2
4
6
0 15 30 45 60 90
placebo
amino acids
time: p<0.001treatment: p=0.009
stress
Salivary cortisol(ng/ml)
5
7
9
11
13
15
0 15 30 45 60 90
placebo
amino acids
time: 0.004treatment: p<0.001
stress
Plasma cortisol(μg/100ml)
Amino acid supplementation
Serotonine and stress
0
50
100
150
200
0 30 45 60
pla
cit
tia
p<0.05
ACTH(pg/ml)
min
Enhancement of stress-induced ACTH release by treatment with citalopram and tianeptine
insulin
020406080
k
basal values
obtaining high quality scientific data with relevance to clinical research
significant improvement of the training process of young researches both from EU and Slovakia
active and broad cooperation among European scientists
„Postdoctoral fellow“Silvia Anette Wein, PhD., GermanyLaboratory Chief : Ing. E. Šeböková, DSc.
„Postdoctoral fellow“Ibolya Bodnár, PhD., HungaryLaboratory Chief : RNDr. R. Kvetňanský, DSc.
Long-term stays in the Centre
„Postdoctoral fellow“Sven Mostböck, PhD., Austria Laboratory Chief: Ing. J. Brtko, PhD.
„Senior Visitor“
Prof. Barbro B. Johansson, M.D., Sweden
Long-term stays in the Centre
„PhD. Student“Aikaterini Makatsori, M.D., GreeceTutor : Assoc. Prof. D. Ježová
Long-term stays in the Centre
1. Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D.:Modulation of neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release inhibiting drug lamotrigine. Neuroendocrinology 79:34-42, 2004
2. Makatsori A., Duncko R., Schwendt M., Moncek F., Johansson B. B., Jezova D.: Voluntary wheel running modulates glutamate receptor subunit gene expression and stress hormone release in Lewis rats. Psychoneuroendocrinology 28: 702-714, 2003
3. Makatsori A., Jezova D.: Drug-induced fetal damage and the consequences for neuroendocrine function. Pediatric Endocrinology vol. 2: 53-61, 2003
4. Jezova D., Skultetyova I., Makatsori A., Moncek F., Duncko R.: Hypothalamo-pituitary-adrenocortical axis function and hedonic behavior in adult male and female rats prenatally stressed by maternal food restriction. Stress 5: 177-183, 2002
5. Schwendt M., Dunčko D., Makatsori A., Johansson B.B., Ježová D.:Involvment of glutamate neurotransmission in the development of excessive wheel running in Lewis rats. Neurochemical Research ,28: 653-657,2003
6. Bakos J., Duncko R., Makatsori A., Pirnik Z., Kiss A., Jezova D.: Prenatal immune challenge affects growth, behavior, and brain dopamine in the offspring. Annals of the New York Academy of Sciences (in press, 2004)
7. Pistovcakova J., Makatsori A., Sulcova A., Jezova D.: Felbamate reduces hormone release and locomotor hypoactivity induced by repeated stress of social defeat in mice. European Neuropsychopharmacology (in submission)
8. Makatsori A., Dubovicky M., Ujhazy E., Duncko R., Bakos J., Jezova D.: Neuroendocrine changes in adult female rats prenatally exposed to phenytoin. Toxicology and Applied Pharmacology (in submission)
9. Jezova D., Makatsori A., Duncko R., Moncek F., Jakubek M.: High trait anxiety in healthy subjects: association with low neuroendocrine responses during psychosocial stress. Progress in Neuro-psychopharmacology and Biological Psychiatry (in submission)
List of publications of the PhD student A. Makatsori, M.D.
obtaining high quality scientific data with relevance to clinical research
significant improvement of the training process of young researches both from EU and Slovakia
active and broad cooperation among European scientists
X. International Congress of the Czech and Slovak Neurochemical Society
„NEUROSCIENCE IN THE THIRD MILLENNIUM“
June 1 – 5, 2002, ČASTÁ, SLOVAKIA
Institute of Experimental EndocrinologyCentre of Excellence supported by European Commission
Bratislava, Slovakia
www.neuromeeting2002.savba.sk
Organised by
INSTITUTE OF EXPERIMENTAL ENDOCRINOLOGYCentre of Excellence of European Commission, Bratislava, Slovakia
Slovak Academy of Sciences
June 28 - July 3, 2003 Smolenice Castle, Slovakia
Work package number and title: WP 14
Twinning and Networking with successful Centres of Excellence Objectives originally planned - To increase the cooperation among scientists in the Central European region.- To increase the scientific quality of meetings and training programs organized by successful Centres and other European laboratories
Change in the quality of the Centre
The Centre • has changed into an institution that has more foreign
scientists involved in local research than the number of local researchers working abroad
• has become an international centre for the education of young scientists
STRESSNUTS
Work package number and title: WP 12
Twinning and Networking with Institute of Adaptive and Space Physiology, University of Graz,Graz, Austria Objectives originally planned - To increase the quality of the research of the Centre in the field of renin-angiotensin systems and body fluids regulation- To apply the experience of the twinning laboratory in the research programme of the Centre
Twinning Laboratory
Institute of Adaptive and Space Physiology and Department of Physiology, University of Graz, Austria
Publications and papers
Hinghofer-Szalkay H., Loder I., Pilz K., Rossler A., Jezova D. : Gravity and cardiovascular regulation: Combined stimulation paradigm. Journal of Gravitational Physiology 10: 89-90, 2003
Makatsori A., Duncko R., Moncek F., Loder I., Katina S., Jezova D. : Modulation of
neuroendocrine response and non-verbal behavior during psychosocial stress in healthy volunteers by the glutamate release-inhibiting drug lamotrigine. Neuroendocrinology 79: 34-42, 2004
Hinghofer-Szalkay H, Haditsch B, Loder I, Pilz K, Rössler A, Jezova D. Head down tilt at –6° to –24° can neutralize the cardiovascular effects of LBNP at –15 or –35 mmHg. Aviat Space Environ Med 2004; 75: 947-51
Hinghofer-Szalkay HG, László Z, Passath H, Pilz K, Rössler A, Jezova D., Scharfetter H.: Sodium intake does not influence bioimpedance.derived extracellular volume loss in head-down rest. Aviat. Space Environ. Med. 75: 1036-41, 2004
Loder I., Roessler A., Wurzinger G., Duncko R., Jezova D., Hinghofer-Szalkay H.: Adrenomedullin and elements of orthostatic competence after 41 hours water submersion in men. Life Sci. 2004
Stress model
relaxing phase
anticipation 15 min
speech 15 min
relaxing phase
Stress model
Postural changes during public speech tests contribute to the stress response Martin Mlynarik a, Aikaterini Makatsori a, Iveta Matyiova a, Helmut G. Hinghofer-Szalkay b,Daniela Jezova a,*
a Laboratory of Pharmacological Neuroendocrinology, Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, Bratislava 83306, Slovakiab Institute for Adaptive and Spaceflight Physiology, Wormgasse 9, A-8010 Graz, Austria Running head: Postural changes and stress response