ANTI CARDIOLIPIN ANTIBODY

PRESENTED BY ASHARUN 11BBC003

ACA is form of antibody directed against CARDIOLIPIN.

Cardiolipin is an important component of the inner

mitochondrial membrane, where it constitutes about 20% of

the total lipid composition.

when immune system recognize cardiolipin as a foreign

substance, ACA is produced and results in auto immune

disorder.

WHAT IS ACA?

Manual method for ACA test

The ACA test detects the autoantibodies

present in an individual’s blood serum. The common test used for detecting and

quantifying ACAs is ENZYME LINKED IMMUNOSORBANT ASSAY(ELISA).

1st step :

Sample addition – 490µl diluent + 10µl sample

Incubation – 30 minutes at room temperature.

Washing – 3 times with wash buffer either manually or automatically.

2nd step :

Addition of conjugate – 100µl conjugate

Incubation – 30 minutes at room temperature.

Washing – washed with wash buffer.

3rd step :Adding TMB solution – 100 µl substrate

Adding stop solution - Stopping the

enzymatic reaction.

MEASUREMENT

Qualitative results provide a simple positive or negative result.

In quantitative ELISA, measured at 420nm in colorimeter.

oAnti cardiolipin antibodies are found in several diseases,

including syphilis, antiphospholipid syndrome, livedoid

vasculitis, vertebrobasilar insufficiency, and Systemic

Lupus Erythematosus(SLE).

oThey are form of anti-mitochondrial antibody.

o In SLE, anti-DNA antibodies and anti-cardiolipin

antibodies may be present individually or together

CLINICAL SIGNIFICANT

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