Post on 11-Mar-2021
Anetta Kuczyńska
Institute of Plant Genetics,
Polish Academy of Science,
Poznań, Poland
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
POLAPGEN-BD: Biotechnological tools for breeding cereals
with increased resistance to drought
Task 2. Genetic maps and the location of QTLs associated with
barley resistance to the water deficiency
Project carried out under Innovative Economy Programme 2007-2013, Action 1.3,
Subaction 1.3.1. within the subject „Biological progress in agriculture and
environment protection”
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
EPPN Plant Phenotyping Symposium, Barcelona 2015
THE AIM OF THE PROJECT
1. The general aim of the project was to enhance
competitiveness of breeding companies
producing cereal varieties and development of
biotechnological services in Poland. This is the
action that will lead to a biological progress.
2. Environmental protection.
3. Creating prototype biotechnological services,
which could be provided to breeders and
agricultural producers.
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
A system approach is achieved by adopting a model of tolerance to drought
stress containing ecophysiological, morphological, anatomical, metabolic,
proteomic, and molecular levels considered in the context of genetics.
TOPICS & PARTNERS
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
THE AIM OF THE STUDY
Mapping of quantitative trait loci (QTL) affecting yield and its
components in European/Syrian spring barley (Hordeum vulgare L.)
cross combinations under optimal and water shortage conditions.
genotyping
From
phenotyping QTL
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
PLANT MATERIAL
Three populations consisted of 100 RILs (F8) each, generated via SSD
technique in POLAPGEN project:
Maresi
(German, semidwarf
malting variety)
Cam/B1/CI 08887/CI
05761 (Syrian variety)
Maresi x Cam/B1/CI, Lubuski x Cam/B1/CI, Georgie x Harmal.
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
RIL POPULATIONS - OBTAINING
Fig. Steps in the procedure of obtaining RIL combined with in vitro culture: a) spikes and immature kernels from
which the embryos were dissected; b) embryos placed on B5 medium; c) embryos developed after 4 days of
culture; d) plantlets after 8 days of in vitro culture; e) plants transplanted in pots; f) spike plants after flowering stage
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
1. pF 2.2-3.0 – optimal conditions
GREENHOUSE EXPERIMENT
• the soil was taken from the Grabów Experimental Station (E 21º 39’,
N 51º 21’);
• the soil texture and properties were characterized by the Institute of
Agrophisics, Lublin;
• fertilization according to the Institute of Soil Science and Plant Cultivation,
Puławy;
• water retention curve pF;
The pF value is defined as a logarithm of the pressure p
(expressed in cm of water head) necessary for water removal
from soil capillaries.
– levels of soil moisture:
2. pF 3.2-3.6 – water deficit
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
EARLY DROUGHT (TREATMENT I)
Water shortage was imposed at the three leaf stage
(phase 13 in the BBCH scale) and maintained for 10 days.
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
LATE DROUGHT (TREATMENT II)
Water shortage was imposed at the flag leaf stage (phase 37 in the
BBCH scale) and maintained for 14 days.
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
MEASURMENTS OF SOIL MOISTURE
pF 3,2 3,4 3,6 3,8 4,2
Humidity %w/w
8,0 6,75 5,7 4,75 3,6
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
PHENOTYPING Traits
1. Grain weight per main spike (g)
2. Grain weight per lateral spike (g)
3. Grain weight per plant (g)
4. 1000-grain weight (g)
5. Length of main spike (cm)
6. Length of lateral spike (cm)
7. Number of grains per main spike
8. Number of grains per lateral spike
9. Number of spikelets per main spike
10. Number of spikelets per lateral spike
11. Fertility of main spike
12. Fertility of lateral spike
13. Length of main stem (cm)
14. Number of productive tillers per plant
15. Number of tillers per plant
16. Productivity of tillers
17. Straw weight per plant (g)
18. Heading stage (days)
Average
of 10 plants
Plants secured for biometric
observations
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
1H.2
8670-3880.0ABC09402-1-2-2751.23220-7232.1
6195-21373.74456-4435.0
3101-111 7372-12537.6
5494-31612.5Bmac021313.72609-35014.6
2901-113725.2
1906-42929.64226-570 5318-43630.1GBM121630.66792-194531.6Bmac006332.55194-111834.4
GBM1334 6081-85036.9
2265-36339.3596-103541.45381-195042.96382-396 409-16432314-1412 3277-446
43.3
5547-29447.011603-44548.010070-143548.87299-18348.9ABC16273-1-1-4849.5Bmac0032 1386-208850.76142-154452.04665-88252.26655-97853.4
2036-102757.1
1670-36961.0
1H_1
713-9720.0
Bmac01541.7
7083-7176.834-4727.22881-9357.56250-10569.0
9757-58212.7ABC08077-pHv131-0213.18867-459 GBM516213.71347-106114.1
ABC11290-sfp44-0618.0
13589-17520.9
4625-141327.25336-40027.3HVHVA127.53786-220427.8
4962-129531.35048-168532.6
557-1297 6547-136334.4
4978-103036.1
ConsensusGBS0554-4 ABC05061-1-1-1593671-59
43.3
9022-54343.7WMC1E844.0Bmag057944.6
4057-2114 13095-18747.7
1H_3
8670-3880.0ABC09402-1-2-2751.23220-7232.1
6195-21373.74456-4435.0
3101-111 7372-12537.6
5494-31612.5Bmac021313.72609-35014.6
2901-113725.2
1906-42929.64226-570 5318-43630.1GBM121630.66792-194531.6Bmac006332.55194-111834.4
GBM1334 6081-85036.9
2265-36339.3596-103541.45381-195042.96382-396 409-16432314-1412 3277-446
43.3
5547-29447.011603-44548.010070-143548.87299-18348.9ABC16273-1-1-4849.5Bmac0032 1386-208850.76142-154452.04665-88252.26655-97853.4
2036-102757.1
1670-36961.0
1H_1
713-9720.0
Bmac01541.7
7083-7176.834-4727.22881-9357.56250-10569.0
9757-58212.7ABC08077-pHv131-0213.18867-459 GBM516213.71347-106114.1
ABC11290-sfp44-0618.0
13589-17520.9
4625-141327.25336-40027.3HVHVA127.53786-220427.8
4962-129531.35048-168532.6
557-1297 6547-136334.4
4978-103036.1
ConsensusGBS0554-4 ABC05061-1-1-1593671-59
43.3
9022-54343.7WMC1E844.0Bmag057944.6
4057-2114 13095-18747.7
1H_3
3452-13550.09490-8430.8
HVM366.0
5880-254710.71865-39611.2GBM121411.67766-49212.48787-145912.77747-105612.9Bmag069216.0
1447-46426.2
3709-71628.4
6338-682 2964-382ABC03253-1-2-279
30.4
2651-177432.61341-84133.1
2417-92440.93122-90947.25379-595 ABC12560-1-1-42147.45113-62447.76384-866 GBM145947.92580-145648.4Bmac009351.25184-116353.24467-37453.3EBmac0684b54.9Bmag072055.77489-44256.210489-58658.87729-56560.02284-173860.34037-91662.8334-116463.52809-27164.5Bmag071164.84377-57165.54164-17665.98065-120366.85644-20670.07549-78270.5ABC09163-1-3-31372.26117-150772.5ConsensusGBS0705-180.13469-115281.87612-37582.78632-180983.46024-109583.5Bmag012584.16752-101384.82020-53989.75837-42793.1ABC10472-1-2-24793.6682-76793.85347-58593.911660-36595.6EBmatc003998.33206-67099.4ConsensusGBS0348-2100.0ABC13569-1-1-107100.8ABC04580-1-4-420101.42688-1022102.6111-499102.98501-449103.2GBM1309103.46996-838104.111979-928105.13180-1771106.714832-296108.6866-442108.95088-59109.0871-462109.27487-390109.4ABC01334-1-1-55109.5868-675109.74266-387111.5ABC09941-1-1-100 3000-1074113.2ConsensusGBS0379-1113.5EBmag0793114.2ABC01791-1-1-110 HVM54114.4GBM1469114.81250-923115.22464-1228 6328-736115.34472-658115.51613-291116.52822-739 285-2932117.53608-2133 6990-661121.27826-869122.23179-497122.43840-348122.5ABC10785-1-1-82122.66157-1233122.74241-445122.84240-749 252-556123.1GBM1462127.1
5419-760142.79910-427 6419-1680143.21449-1103 1344-930144.9
2H_6
1H.1 2H GBM12800.0
ConsensusGBS0194-14.61831-241 13924-4035.75533-3406.13344-11476.6GBM14506.77060-12376.95945-7487.61499-2908.83646-19848.97044-7059.41440-114812.44701-239516.02995-71717.2EBmac070519.43886-31320.75646-56822.23688-129124.84593-200729.91325-31130.84844-173731.16156-139932.12765-40632.36634-26335.76805-1110 3933-107738.04035-9938.34105-141738.7Bmag082842.1GBM144442.32838-66342.6Bmag013144.96491-29545.3ABC13089-1-2-47846.64728-173546.9ABC05866-1-1-31147.1ABC04375-1-1-17347.25657-1187 ABC05852-1-2-6586818-411
47.3
ConsensusGBS0697-1 3354-1212861-1941 ABC14167-1-2-250Bmag0138a 7716-7219658-234 10248-9543143-1276 2588-2294707-421
47.4
2288-126 6171-9565020-145 ConsensusGBS0471-11630-1150 GBM12137728-341
47.5
10353-119 2676-2564213-2378
47.6
ABC11783-1-1-99 2066-113347.7Bmag060347.8Bmac020947.9ABC10667-1-1-28848.23892-247249.65038-103552.02804-183252.5ABC08184-2-1-3554.1ABC18717-1-3-21555.9ABC14384-1-1-5356.36364-64556.74184-39357.28180-45057.72616-256057.9ABC19175-1-2-37558.81961-28760.25385-72260.35128-183160.5HVM3360.8GBM149561.4ABC13107-1-3-29961.810317-44861.91827-95862.42372-70362.5ABC10084-1-2-36363.21176-1547 2447-5461639-613
63.4
2677-50163.93470-13465.04618-155965.34150-39865.48722-51270.2HVM6075.5Bmag022577.4GBM109079.5GBM140583.6GBM123384.0Bmag0010b85.5ABC11028-1-1-6486.11898-580 6302-25087.33674-135288.13791-152588.9ABC18746-1-2-10990.86871-94595.27241-55395.71435-67095.87867-58596.61038-75497.3Bmag060698.83315-749101.22500-1514101.75488-1097103.4ABC07496-pHv1343-02103.99282-205104.476-1059104.86716-823105.33965-353105.45260-462105.84026-655107.82335-1614108.7ABC08260-1-1-108109.0ABC06381-1-5-73109.1Bmag0013116.8ABC05919-1-2-157 2847-48511657-398 9618-3722754-1027
123.5
EBmac0541124.9GBM1288132.1EBmag0705137.5
3H_8
ABC13678-1-2-3690.05334-15331.1
Bmag08773.45008-24023.64270-1844.2
7818-96711.0
EBmac070818.35840-65919.4ConsensusGBS0271-220.34787-174620.64737-36821.8
ABC38781-pHv2346-0128.04643-86728.87782-41029.6ConsensusGBS0632-329.9
265-122934.28752-52335.72346-31836.6GBM142037.4
scssr2553842.3
3H_5
3H.1
GBM12800.0
ConsensusGBS0194-14.61831-241 13924-4035.75533-3406.13344-11476.6GBM14506.77060-12376.95945-7487.61499-2908.83646-19848.97044-7059.41440-114812.44701-239516.02995-71717.2EBmac070519.43886-31320.75646-56822.23688-129124.84593-200729.91325-31130.84844-173731.16156-139932.12765-40632.36634-26335.76805-1110 3933-107738.04035-9938.34105-141738.7Bmag082842.1GBM144442.32838-66342.6Bmag013144.96491-29545.3ABC13089-1-2-47846.64728-173546.9ABC05866-1-1-31147.1ABC04375-1-1-17347.25657-1187 ABC05852-1-2-6586818-411
47.3
ConsensusGBS0697-1 3354-1212861-1941 ABC14167-1-2-250Bmag0138a 7716-7219658-234 10248-9543143-1276 2588-2294707-421
47.4
2288-126 6171-9565020-145 ConsensusGBS0471-11630-1150 GBM12137728-341
47.5
10353-119 2676-2564213-2378
47.6
ABC11783-1-1-99 2066-113347.7Bmag060347.8Bmac020947.9ABC10667-1-1-28848.23892-247249.65038-103552.02804-183252.5ABC08184-2-1-3554.1ABC18717-1-3-21555.9ABC14384-1-1-5356.36364-64556.74184-39357.28180-45057.72616-256057.9ABC19175-1-2-37558.81961-28760.25385-72260.35128-183160.5HVM3360.8GBM149561.4ABC13107-1-3-29961.810317-44861.91827-95862.42372-70362.5ABC10084-1-2-36363.21176-1547 2447-5461639-613
63.4
2677-50163.93470-13465.04618-155965.34150-39865.48722-51270.2HVM6075.5Bmag022577.4GBM109079.5GBM140583.6GBM123384.0Bmag0010b85.5ABC11028-1-1-6486.11898-580 6302-25087.33674-135288.13791-152588.9ABC18746-1-2-10990.86871-94595.27241-55395.71435-67095.87867-58596.61038-75497.3Bmag060698.83315-749101.22500-1514101.75488-1097103.4ABC07496-pHv1343-02103.99282-205104.476-1059104.86716-823105.33965-353105.45260-462105.84026-655107.82335-1614108.7ABC08260-1-1-108109.0ABC06381-1-5-73109.1Bmag0013116.8ABC05919-1-2-157 2847-48511657-398 9618-3722754-1027
123.5
EBmac0541124.9GBM1288132.1EBmag0705137.5
3H_8
ABC13678-1-2-3690.05334-15331.1
Bmag08773.45008-24023.64270-1844.2
7818-96711.0
EBmac070818.35840-65919.4ConsensusGBS0271-220.34787-174620.64737-36821.8
ABC38781-pHv2346-0128.04643-86728.87782-41029.6ConsensusGBS0632-329.9
265-122934.28752-52335.72346-31836.6GBM142037.4
scssr2553842.3
3H_5
3H.2
6133-7740.01996-6520.87385-7631.33026-10111.4ABC14522-1-8-3503.0GBM12214.23551-25376.52533-7737.42055-9479.0HVM4010.22065-313511.23432-29011.5GBM132317.11593-159717.62129-192818.21595-110718.86589-121121.9917-52223.02769-124523.38901-144623.814765-38830.41227-1323 10255-52930.75726-41434.93193-67135.3ABC09891-1-1-2835.55475-135536.0Bmac0303c36.56249-57236.96215-539 3190-6445298-828
37.1
4636-138037.2454-1502 3984-192037.4788-842 EBmac0906HVM03
37.7
1077-45738.0Bmac018138.37942-94839.5ABC10254-1-2-25040.01169-94440.32776-63741.8294-956 3078-219041.95889-1154 1304-47842.010346-9242.13716-91042.62274-122643.69208-91944.1Bmag049044.73127-27345.1GBM150946.75273-89447.7ABC09662-1-3-35247.910321-36449.14139-88849.9ABC09432-1-1-16052.26464-111552.411293-49053.93549-74355.1ABC14714-1-1-16256.3ConsensusGBS0589-156.9GBM129957.14407-134459.53416-69259.96172-773 EBmac077561.84807-132863.98463-46665.22196-19565.6ConsensusGBS0461-368.64564-60470.9ABC20090-1-1-27571.42400-95672.04039-168672.84986-121473.64133-60174.25245-30476.5ABC12093-1-2-23179.74535-136680.22574-41080.44361-186780.6EBmac0701 EBmac067981.4ConsensusGBS0288-185.541-69586.76519-81288.02614-152288.2WMS693.1ABC12417-1-1-4693.74160-136593.9ABC02813-1-4-326 4773-100994.14152-573 1954-119897.1HVM6797.32297-125098.21659-301 1010-126898.62878-574100.1954-1377101.1GBM1453101.2
4H_6
4H
ConsensusGBS0136-70.02057-4122.85148-14143.61872-13724.37185-3706.01769-545 2795-170712.61066-211013.9GBM127019.92188-42520.5885-10421.7GBM135522.45771-9124.03164-138625.412210-48028.61588-53730.06964-41432.73580-33134.95448-29836.22176-89138.53379-103740.04445-191140.24191-26841.21041-144141.4Bmag080741.66018-23541.83764-66641.9ABC04725-1-1-25442.2Bmag017345.06854-30947.62298-152647.94258-1498 ABC02895-1-4-23148.18220-1223 4313-48211016-603
48.6
4895-145 210-45049.51490-95949.73656-341 28-22949.81911-5551.0832-104051.1828-545 5873-88051.3397-28852.0851-80452.14721-133552.23658-131052.8ABC13045-1-1-22652.95017-407 2559-17963506-668
53.2
5232-204153.34656-25553.46487-131553.55497-66153.67282-138653.72259-48854.0Bmag000954.1HVM3154.2GBM501255.03348-39556.36205-68356.54203-121056.75554-197156.81565-51457.0ABC10738-1-1-25857.31140-150858.24064-172458.63048-134959.05187-75259.73773-75660.1Bmag061360.7EBmac080663.77361-937 EBmac06024454-1080
64.2
5172-92664.39251-85267.83987-1078 5965-138771.02968-1066 5926-79872.81969-132273.7578-58774.1428-151974.678-107375.0ABC12239-1-1-31675.78504-785 5684-60177.9ConsensusGBS0708-679.14374-60979.59981-66582.11473-111582.7ABC03519-1-3-13283.1ABC08038-1-3-16083.91597-15884.31007-65184.710535-21784.95225-70385.1GBM1087 ConsensusGBS0388-186.0GBM140486.45074-113586.93363-179598.2
6H_2
6H
1800-11010.0
Bmag01201.9
1178-2799.85796-118911.23492-67811.4
2378-49814.7
1894-213218.0
6628-130225.37397-85427.013008-35228.7EBmac075529.913108-41234.1382-262436.44137-79836.92966-62737.36433-12437.5277-93537.78758-56437.8ConsensusGBS0084-137.9ABC11252-1-2-25438.81590-54438.95595-29739.312368-20739.7Bmac015640.03579-70342.38923-70743.83818-112345.61437-68746.3Bmag013546.9
7H_16
1404-640.0
GBM13268.27634-6598.48365-4549.23313-14439.3452-4989.6EBmac06039.82124-984 8142-67610.25777-35410.3ABC03024-1-3-36812.06353-52415.01213-195916.02585-290119.8398-124420.1Bmag091420.9AF022725A23.5ConsensusGBS0356-124.2scssr07970a26.4GBM146426.7239-776 1792-3725467-1663 ABC10361-1-3-3168390-328
28.4
4679-159430.112239-66230.4943-310732.72669-101234.37810-113 5695-92235.6EBmatc001636.7HVCMA37.2497-38637.6ABC14535-1-1-7540.4Bmac018741.4ABC04803-1-1-39241.8
9820-45548.61107-39249.11518-62449.6
ABC09320-1-1-11258.0Bmac0167 ABC06618-1-2-3141997-77 EBmac08271290-729 5480-826
58.7
5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1
5212-140975.1
11442-59286.7
7H_15
9728-10980.0GBM11261.73359-11181.8
2148-4983.6
Bmag020612.3
6394-944 1660-34713.8HVM04 6517-60214.9
1073-91621.5
6093-57225.03187-107325.8
7H_5
1800-11010.0
Bmag01201.9
1178-2799.85796-118911.23492-67811.4
2378-49814.7
1894-213218.0
6628-130225.37397-85427.013008-35228.7EBmac075529.913108-41234.1382-262436.44137-79836.92966-62737.36433-12437.5277-93537.78758-56437.8ConsensusGBS0084-137.9ABC11252-1-2-25438.81590-54438.95595-29739.312368-20739.7Bmac015640.03579-70342.38923-70743.83818-112345.61437-68746.3Bmag013546.9
7H_16
1404-640.0
GBM13268.27634-6598.48365-4549.23313-14439.3452-4989.6EBmac06039.82124-984 8142-67610.25777-35410.3ABC03024-1-3-36812.06353-52415.01213-195916.02585-290119.8398-124420.1Bmag091420.9AF022725A23.5ConsensusGBS0356-124.2scssr07970a26.4GBM146426.7239-776 1792-3725467-1663 ABC10361-1-3-3168390-328
28.4
4679-159430.112239-66230.4943-310732.72669-101234.37810-113 5695-92235.6EBmatc001636.7HVCMA37.2497-38637.6ABC14535-1-1-7540.4Bmac018741.4ABC04803-1-1-39241.8
9820-45548.61107-39249.11518-62449.6
ABC09320-1-1-11258.0Bmac0167 ABC06618-1-2-3141997-77 EBmac08271290-729 5480-826
58.7
5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1
5212-140975.1
11442-59286.7
7H_15
9728-10980.0GBM11261.73359-11181.8
2148-4983.6
Bmag020612.3
6394-944 1660-34713.8HVM04 6517-60214.9
1073-91621.5
6093-57225.03187-107325.8
7H_5
1800-11010.0
Bmag01201.9
1178-2799.85796-118911.23492-67811.4
2378-49814.7
1894-213218.0
6628-130225.37397-85427.013008-35228.7EBmac075529.913108-41234.1382-262436.44137-79836.92966-62737.36433-12437.5277-93537.78758-56437.8ConsensusGBS0084-137.9ABC11252-1-2-25438.81590-54438.95595-29739.312368-20739.7Bmac015640.03579-70342.38923-70743.83818-112345.61437-68746.3Bmag013546.9
7H_16
1404-640.0
GBM13268.27634-6598.48365-4549.23313-14439.3452-4989.6EBmac06039.82124-984 8142-67610.25777-35410.3ABC03024-1-3-36812.06353-52415.01213-195916.02585-290119.8398-124420.1Bmag091420.9AF022725A23.5ConsensusGBS0356-124.2scssr07970a26.4GBM146426.7239-776 1792-3725467-1663 ABC10361-1-3-3168390-328
28.4
4679-159430.112239-66230.4943-310732.72669-101234.37810-113 5695-92235.6EBmatc001636.7HVCMA37.2497-38637.6ABC14535-1-1-7540.4Bmac018741.4ABC04803-1-1-39241.8
9820-45548.61107-39249.11518-62449.6
ABC09320-1-1-11258.0Bmac0167 ABC06618-1-2-3141997-77 EBmac08271290-729 5480-826
58.7
5138-26559.25-159359.34849-124862.2ABC05818-1-2-27162.82429-192964.1
5212-140975.1
11442-59286.7
7H_15
9728-10980.0GBM11261.73359-11181.8
2148-4983.6
Bmag020612.3
6394-944 1660-34713.8HVM04 6517-60214.9
1073-91621.5
6093-57225.03187-107325.8
7H_5
7H.1
7H.2
7H.3
9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188
17.9
4166-1090 8631-46918.17276-942 ConsensusGBS0527-52664-314 Bmac0306aBmac0113 4154-3643012-1125
18.2
5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318
18.3
1215-862 8201-8618.49766-78718.5ConsensusGBS0654-418.62283-2913 7741-52718.76820-58618.94930-160419.012045-83 ABC09365-1-3-3787140-595 2146-2256
19.3
4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9
5H_5
3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4
10207-10248.1
847-139612.7
ABC01741-1-4-29915.32594-17216.44684-77517.1
3997-79618.8
5H_4
GBM14260.0scssr15334 GBM15064.83928-5135.79745-62810.6GBM148311.23685-89414.7Bmag022315.03333-120915.26833-65815.8370-44316.2Bmag081221.8956-47322.2ABC14689-1-9-39923.310318-57225.03398-163 4706-94026.810669-18827.36315-91428.5ABC15617-1-3-26528.82208-27929.56913-123530.73478-102431.4ABC11221-1-3-41036.3139-126343.93800-81444.23877-192344.512756-23344.7GBM500845.29566-1214 GBM143845.4ConsensusGBS0531-145.610047-33848.32352-27748.67167-46648.93985-82449.15844-101152.2211-25952.31164-268354.47639-12254.91697-63655.55571-64056.02091-63956.34795-78257.0ABC03900-1-2-406 7523-44058.41394-122258.6314-55959.05156-73759.56553-32860.2ConsensusGBS0138-261.5GMS06162.65286-48663.76050-162564.02520-718 2395-208364.33412-57964.7ABC04352-pHv108-0167.0447-8867.84346-84069.0407-259 1461-52970.0ConsensusGBS0086-571.51306-40872.03477-124872.92746-150173.5GMS02774.3EBmac082474.54050-2006 203-11288682-406
76.1
ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050
78.2
3759-138579.15126-1311 3443-1234Bmag0222
81.9
ConsensusGBS0451-182.9GBM149083.15925-56584.611470-47885.19864-22585.24015-115385.33720-52 5163-89685.47342-53585.62867-37385.93931-143487.03390-20588.3ABC07987-1-6-27088.65893-128589.4ConsensusGBS0269-190.45428-14690.93391-213793.2485-136993.62167-335 4066-32893.7ABC09278-1-4-6993.96735-75494.44845-123 4117-47294.5552-188 585-34297.16781-107397.93362-64498.14103-138698.32244-3247100.26761-490100.95145-1355101.62144-852102.110386-329102.35219-773103.04658-1237104.22493-660 6116-709105.16851-867105.22978-938108.1scssr03907109.512925-332116.0
5H_2
9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188
17.9
4166-1090 8631-46918.17276-942 ConsensusGBS0527-52664-314 Bmac0306aBmac0113 4154-3643012-1125
18.2
5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318
18.3
1215-862 8201-8618.49766-78718.5ConsensusGBS0654-418.62283-2913 7741-52718.76820-58618.94930-160419.012045-83 ABC09365-1-3-3787140-595 2146-2256
19.3
4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9
5H_5
3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4
10207-10248.1
847-139612.7
ABC01741-1-4-29915.32594-17216.44684-77517.1
3997-79618.8
5H_4
GBM14260.0scssr15334 GBM15064.83928-5135.79745-62810.6GBM148311.23685-89414.7Bmag022315.03333-120915.26833-65815.8370-44316.2Bmag081221.8956-47322.2ABC14689-1-9-39923.310318-57225.03398-163 4706-94026.810669-18827.36315-91428.5ABC15617-1-3-26528.82208-27929.56913-123530.73478-102431.4ABC11221-1-3-41036.3139-126343.93800-81444.23877-192344.512756-23344.7GBM500845.29566-1214 GBM143845.4ConsensusGBS0531-145.610047-33848.32352-27748.67167-46648.93985-82449.15844-101152.2211-25952.31164-268354.47639-12254.91697-63655.55571-64056.02091-63956.34795-78257.0ABC03900-1-2-406 7523-44058.41394-122258.6314-55959.05156-73759.56553-32860.2ConsensusGBS0138-261.5GMS06162.65286-48663.76050-162564.02520-718 2395-208364.33412-57964.7ABC04352-pHv108-0167.0447-8867.84346-84069.0407-259 1461-52970.0ConsensusGBS0086-571.51306-40872.03477-124872.92746-150173.5GMS02774.3EBmac082474.54050-2006 203-11288682-406
76.1
ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050
78.2
3759-138579.15126-1311 3443-1234Bmag0222
81.9
ConsensusGBS0451-182.9GBM149083.15925-56584.611470-47885.19864-22585.24015-115385.33720-52 5163-89685.47342-53585.62867-37385.93931-143487.03390-20588.3ABC07987-1-6-27088.65893-128589.4ConsensusGBS0269-190.45428-14690.93391-213793.2485-136993.62167-335 4066-32893.7ABC09278-1-4-6993.96735-75494.44845-123 4117-47294.5552-188 585-34297.16781-107397.93362-64498.14103-138698.32244-3247100.26761-490100.95145-1355101.62144-852102.110386-329102.35219-773103.04658-1237104.22493-660 6116-709105.16851-867105.22978-938108.1scssr03907109.512925-332116.0
5H_2
9100-9780.04334-48210.63498-76114.14570-59114.3GBM122917.5Bmac016317.6ABC10705-1-1-26317.78118-100517.8ABC08327-1-1-353 152-32912005-188
17.9
4166-1090 8631-46918.17276-942 ConsensusGBS0527-52664-314 Bmac0306aBmac0113 4154-3643012-1125
18.2
5565-1908 8320-9554517-217 1286-9904392-450 5850-23478852-318
18.3
1215-862 8201-8618.49766-78718.5ConsensusGBS0654-418.62283-2913 7741-52718.76820-58618.94930-160419.012045-83 ABC09365-1-3-3787140-595 2146-2256
19.3
4565-1583 6367-45319.62505-85019.92482-12622.14234-194426.07839-633 2906-117726.47504-48526.9
5H_5
3417-14510.05206-7870.6166-1541.77310-9963.0scssr023064.4
10207-10248.1
847-139612.7
ABC01741-1-4-29915.32594-17216.44684-77517.1
3997-79618.8
5H_4
GBM14260.0scssr15334 GBM15064.83928-5135.79745-62810.6GBM148311.23685-89414.7Bmag022315.03333-120915.26833-65815.8370-44316.2Bmag081221.8956-47322.2ABC14689-1-9-39923.310318-57225.03398-163 4706-94026.810669-18827.36315-91428.5ABC15617-1-3-26528.82208-27929.56913-123530.73478-102431.4ABC11221-1-3-41036.3139-126343.93800-81444.23877-192344.512756-23344.7GBM500845.29566-1214 GBM143845.4ConsensusGBS0531-145.610047-33848.32352-27748.67167-46648.93985-82449.15844-101152.2211-25952.31164-268354.47639-12254.91697-63655.55571-64056.02091-63956.34795-78257.0ABC03900-1-2-406 7523-44058.41394-122258.6314-55959.05156-73759.56553-32860.2ConsensusGBS0138-261.5GMS06162.65286-48663.76050-162564.02520-718 2395-208364.33412-57964.7ABC04352-pHv108-0167.0447-8867.84346-84069.0407-259 1461-52970.0ConsensusGBS0086-571.51306-40872.03477-124872.92746-150173.5GMS02774.3EBmac082474.54050-2006 203-11288682-406
76.1
ConsensusGBS0397-16 1204-110476.57337-388 603-726054-1050
78.2
3759-138579.15126-1311 3443-1234Bmag0222
81.9
ConsensusGBS0451-182.9GBM149083.15925-56584.611470-47885.19864-22585.24015-115385.33720-52 5163-89685.47342-53585.62867-37385.93931-143487.03390-20588.3ABC07987-1-6-27088.65893-128589.4ConsensusGBS0269-190.45428-14690.93391-213793.2485-136993.62167-335 4066-32893.7ABC09278-1-4-6993.96735-75494.44845-123 4117-47294.5552-188 585-34297.16781-107397.93362-64498.14103-138698.32244-3247100.26761-490100.95145-1355101.62144-852102.110386-329102.35219-773103.04658-1237104.22493-660 6116-709105.16851-867105.22978-938108.1scssr03907109.512925-332116.0
5H_2
5H.3
5H.1
5H.2
1H 2H 3H 4H 5H 6H 7H
A consensus linkage map of barley constructed using three
RIL mapping populations and used for QTL analysis.
Mikołajczak K. et al. Quantitative trait loci for yield and yield-related traits in spring
barley populations derived from crosses between European and Syrian cultivars.
under review
EXPERIMENTS CARRIED OUT IN THE GREENHOUSE
EPPN Plant Phenotyping Symposium, Barcelona 2015
Variation of the spike morphology
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
EXPERIMENTS CARRIED OUT IN THE FIELD
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
Setup experiment 1436PK
Phenotyping of 95 RIL barley lines plus 4 control varieties
8x replicates (2xcarrier per line; 4-pot config per carrier)
2x treatment conditions (control normal watering, stress conditions no watering)
Pre-cultivation phase: 4th of August – 3rd of September 2014
04.08.2014 Sowing of seeds in 96-well tray, soil mixture (Substrate 2 + Sand, 4:1)
24x seeds of each genotype distributed over 24x trays (1xreplicate/tray)
Greenhouse climate conditions:
16°C day/14°C night temperature
>65% relative humidity
Long day conditions (6.00-22.00) additional light
Continuous watering of trays as required (manually done by gardeners)
Transplanting of the plants in individual pots
20.08.2014 Transplanting of single plants in 3.5L pots for LemnaTec system
Soil mixture (Substrate 2 + Sand, 4:1)
Greenhouse climate conditions:
16°C day/14°C night temperature
>65% relative humidity
Long day conditions (6.00-22.00) additional light
Continuous watering of pots as required (manually done by gardeners)
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
Phenotyping: 3rd of September – 8th October 2014
01.09.2014 Transfer pots from greenhouse to LemnaTec phenotyping facilities
28das (days after sowing)
Greenhouse climate conditions:
20°C day/16°C night temperature
>65% relative humidity
Long day conditions (6.00-22.00) additional light
Automatically watering based on watering regime (target weight)
Start watering 03.09.2014
Start imaging 04.09.2014
08.09.2014 Start stress (35das)
No/low watering to 20% field capacity
Normal watering 70% FC (control)
22.09.2014 Start recovery phase
Adding 300ml absolute watering to each pot
23.09.2014 additional target watering to reach 90% FC
25.09.2014 Fertilization, Combo Hakaphos blau (200ml absolute volume per plant)
01.10.2014 Fertilization, Combo Hakaphos blau (200ml absolute volume per plant)
Rotation of the carriers: each day for one lane, every 3rd day in between the lane for 11 positions
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
All lines (RILs, varieties) (left) and varieties only (right)
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
All lines (RILs, varieties) (left) and varieties only (right)
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
All lines (RILs, varieties) (left) and varieties only (right)
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
Post-cultivation: from 7th of October 2014
07.10.2015 Finish phenotyping, transfer of a selection of plants
(around 800 plants) for post-cultivation, harvest
Fig. Biometric analysis of spikes:
A – main spikes collected from a pot;
B – measurement of spikelets number per spike;
C – measurement of spike length
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
Principal component biplot for traits observed after harvest; green – lines non-stressed, red – lines stressed
PC1 (36.34%)
PC
2 (
15.2
8%
)
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
Institute of Plant Genetics Polish Academy of Science:
Maria Surma
Tadeusz Adamski
Paweł Krajewski
Karolina Krystkowiak
Krzysztof Mikołajczak
Piotr Ogrodowicz
Hanna Ćwiek
Leibniz Institute of Plant Genetics and Crop
Plant Research, OT Gatersleben, Germany:
Astrid Junker,
Kathleen Weigelt-Fischer,
Thomas Altmann
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND
EPPN Plant Phenotyping Symposium, Barcelona 2015
INNOVATIVE ECONOMY NATIONAL COHESION STRATEGY
EUROPEAN UNION
EUROPEAN REGIONAL
DEVELOPMENT FUND