Post on 07-Aug-2019
Agilent HPLC Infinity II
Product Introduction
April 23, 2019
1
Vision Scientific
2019 CLICK HERE FOR CONTACT US
Agilent SupportBusiness Development Manager : Etin Diah P.hd
Technical Support Application Engineer
Jakarta
Doan Rizki (12 years experience in pharmaceutical industry)
Wahyu Eko Saputro Cathy (12 years experience in pharmaceutical industry)
Ifan Liza Hidayani S.Si Apt (8 years experience in BaBe Lab)
Risanto Reta Adji
Muhammad Fathurrachman
Fadillah
Randy
Surabaya
Gunarso
Jawa Tengah
Budi Mega Prasetyo
Bandung
Sugiyoto
Technical Support : Qualification (IQOQPV) Service and MaintenanceApplication Support : Method Development, Column Selection and Sample prep
1220Infinity II LC
400 or 600 bar
Affordable Efficiency
The Agilent 1220 Infinity II LC is an affordable, high-quality integrated LC system, putting you on the fast track to efficiency.
1290Infinity II LC
1300 bar
The Benchmark in Efficiency
The Agilent 1290 Infinity II LC embodies the next generation of LC, giving you the ultra-high performance to achieve maximum efficiency.
Agilent InfinityLab LC SeriesModern HPLC for Every Lab – Unlimited Flexibility
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1260Infinity II LC
400 or 600 bar
Everyday Efficiencyin Every Way
The Agilent 1260 Infinity II LC is the trusted platform with the broadest instrument choice, taking you to the next level of efficiency.
1260Infinity II Prime LC
800 bar
Agilent’s most convenient LCfor routine analysis
The1260 Infinity II Prime LC is the most capable and comfortable LC within the 1260 Infinity II LC portfolio.
LC Portofolio
4.6mm columns
3.0mm columns
2.1mm columns
1.0mm columns
Performance
Prime LC Quat 800 bar
Pressure
1290 Quat/Bin
1300 bar
Acquity ARC
Quat 655 bar
1220 Gradient
400 bar
1260 Quat
400 bar
Acquity H-Class
Quat 1034 barAcquity I-Class
Binary 1241
bar
1220 Gradient
600 bar
1260
Quat/Bin 600
bar
AlIiance
Quat 345
bar
5 um columns
3 um columns
< 2 um columns
i-Prominence
Quat 440 bar
i-Nexera Quat
660 bar
Nexera x2
Quat/Bin 1300 bar
HPLC UHPLC
1220 Infinity LC. Agilent Restricted
Isocratic or Gradient Pump (2-Channel):
• flow range: 0.2 - 10 mL/min• low pressure mixing• integrated degasser • flow precision 0.07 % RSD
Manual Injector or Autosampler:•600 bar• injection range: 0.1-100 µL•100 x 2 mL vials or 30 x 6 mL
vials• injection cycle time 50 s•carryover: < 0.05%
Column Oven:•up to 80°C•one column, max. 250 mm
VWD :•80 Hz data rate•wavelength range:190 - 600 nm
(VWD)
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Agilent 1220 Infinity II LCProduct Information
Agilent 1260 Infinity IIOverview
Multicolumn Thermostat4-110 oC
400 or 600 bar Pump
Multisampler432 Vials 2 mL
Sample cooler 4-40oC
Vialsampler 132 Vial 2mL Sample Cooler 4-40 oC
Integrated Column CompartmentUp to 80 oC
User interface – the InfinityLab LC Companion
Many Detectors
Agilent Prime LC/ 1290 Overview
Multicolumn Thermostat4-110 oC
800 or 1300 bar Pump1290 Infinity II pump technologyISET (Intelligent System Emulation Technology)
Multisampler432 Vials 2 mL
Sample cooler 4-40oC
Vialsampler 132 Vial 2mL Sample Cooler 4-40 oC
Integrated Column CompartmentUp to 80 oC
User interface – the InfinityLab LC Companion
Many Detectors
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Quatenary Pump :
• 400 bar or 600 bar
• flow : 0.001 - 10 mL/min
• low pressure mixing
• integrated degasser
• 4 Solvent
Agilent Infinity II LC – 1260 Quatenary Pump
Infinity II Flexible Pump (Prime and 1290)
1260 Infinity II Prime LC
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Built-in Multipurpose Valve
Flexible Pump:
• 800 bar (prime) or 1300 bar (1290)
• flow : 0.001 - 10 mL/min
• low pressure mixing
• integrated degasser
• 4 Solvent
Infinity II Flexible Pump
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Mixing starts here
BlendAssist – A simple tool for online dilution of modifiers and gradient setup
BlendAssist for automatic solvent blending
1260 Infinity II Prime LC
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H2O
+ 0.01%
TFA
MeCN
+ 0.01%
TFA
H2O
+ 0.05%
TFA
MeCN
+ 0.05%
TFA
H2O
+ 0.1%
TFA
MeCN
+ 0.1%
TFA
OR OR
OR...OR...OR....
Time consuming, costly & error-prone !
Infinity II Flexible Pump
1260 Infinity II Prime LC
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H2O
MeCN
H2O
+ 1% TFA
MeCN
+ 1% TFA
H2O
+ 0.2% TFA
MeCN
+ 0.18% TFA
Type in desired
concentration!Run
gradient!
Pure solvent + concentrated stock solution = any concentration you need!
BlendAssist for automatic solvent blending
Infinity II Flexible Pump
1260 Infinity II Prime LC
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BlendAssist for automatic solvent blending
Impurity B
Example 1: Different TFA concentrations
min0 1 2 3 4 5 6 7 8
mAU
0
100
200
300
400
0.05 M buffer
0.025 M buffer
0.01 M buffer
Procainamide
Acetyl-Procainamide
Disopyramide
Quinidine
Example 2: Different Phosphate buffer concentrations
Infinity II Flexible Pump
0.02% TFA
0.20% TFA
0.10% TFA
0.05% TFA
Infinity II Flexible Pump
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*
1290 Infinity II1300 bar - G7104A
Flexible Pump
1290 Infinity II1300 bar - G7120AHigh Speed Pump
Long Life Technology
Long Life Technology
1260 Infinity II800 bar - G7104C
Flexible Pump
Long Life Technology
Intelligent System Emulation Technology
Keep your method and get the same results on the
Infinity II Flexible pump LC
with ISET !!
Intelligent System Emulation Technology
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Time (min) %A %B
0 100 0
0.999 100 0
1 0 100
3 0 100
Single step gradient
Solvent A Solvent B
Commands from SW, programmed gradient
What the column head sees
0min 1min 2min 3min
Delay volume Mixing behavior
How it works - Example
Select vendor
Select system
Select sampleloop
Infinity II Flexible Pump method transfer from Waters Intelligent System Emulation Technology
Agilent Confidential
April 23, 2019
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Infinity II Column Compartment
ICC MCT
Agilent Infinity II ICC (Integrated Column Compartment)
• Temperature Range : 5 °C above ambient to 80 °C• The integrated column compartment is a heatable (no
cooling) • It exists in different versions with volumes of 3 μl and
6 μl.• A tag reader for two columns is optionally available.• It can be clustered with an external valve for column
switching
Easy access column compartment with InfinityLab Quick Connect fittings and new column identification kit
Agilent Infinity II MCT (Multicolumn Thermostat)
• Temperature Range : 4 °C to 110 °C, (minimum 20 °C below ambient)
• The integrated column compartment is a heatable and cooling
• It exists in different versions with volumes of 3 μl and 6 μl.
• A tag reader for four columns is optionally available.
• It can be clustered with an internal valve for column switching
Agilent Confidential
April 23, 2019
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Infinity II Multisampler and Vialsampler
Multisampler Vialsampler
Agilent Infinity II LC - VialsamplerInfinity II Vialsampler
+ Vials 2 mL (66 vials/tray, total 132 vials)+ Integrated column compartment+ Flexibility, great options
+ multi wash (2 solvents)+ Sample cooler 4 °C to 40 °C
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Infinity II Multisampler+ Vials 2 mL (54 vials/tray, 108 vials/rack)+ Well plates available+ High capacity up to 16 WPS (6144 samples)+ Extremely fast (needle to vial)+ High flexibility, great options, great upsell potential
+ dual needle or multi wash (3 solvents)+ Sample cooler 4 °C to 40 °C
+ Highest Capacity per benchspace (2 mL vials maks 432 vials)
96 Well plates, 0.5 ml, polypropylene,
Agilent Infinity II MultiSamplerProduct Information
Drawer
• Holds 2 individual sample containers• LED status indicator
Hydraulic block
Dual-needle park stationsReference vials
Sample Hotel
• Fully scalable• Up to 8 drawers• Flexible for vials & wellplate formats• Convenient access
• Flush pumps• Solvent selections• Metering devices• Injection valve
Agilent Infinity II - Multisampler
96 Well plates, 0.5 ml, polypropylene,
• The dual-needle setup of the 1290 Infinity II Multisamplerenhances flexibility by providing two injectors in a single instrument. You can optimize one path for large volume injections and the other for low delay volumes.
20µl
500µl
Agilent Infinity II - Multisampler
Agilent Infinity II Sample Cooler
SpecificationAmbient Operating Temp. 4-40°C (39,2 - 131°F)Temp. range from 4°C to ambientTemp. settable from 4 – 40°C in 1° C incrementsTemp. Stability +/- 1 °CTemp. Accuracy +/- 2°C
Agilent Infinity II Sample Cooler
1260 Infinity II Variable Wavelength Detector High Data rate Dual wavelength detection Cost effective detection
1260 Infinity II ELSD Lower limit of detection Fewer assays
1260 Infinity II DAD Lower limit of detection Fewer assays (HDR) Maximum method flexibility
1260 Infinity II MWD Lower limit of detection 5 Wavelength detection
Agilent Infinity II LC Detector
1260 Infinity II RID 74 HZ data rate Automatic solvent recycle Automatic Purge
1260 Infinity II FLD (G71721B) On-Line scanning capabilities Spectral Analysis 148 Hz Data rate
Deuterium Lamp
Max-Light Cartridge Cell
Programmableor fixed slit
1024 elementdiode-array
GratingMirror
Optofluidic Waveguides: Max-Light Flow Cells
1260 / 1290 Infinity II DAD
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Agilent 1260 / 1290 Infinity II LCDiode Array UV Detectors (DAD)
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10 mm pathlengthsmall geom. cell volume (13 µL)
high light transmission
60 mm pathlength4 µL σV dispersion volume
Optofluidic waveguides (total internal reflection)
> 10-X Higher Sensitivity 1260/1290 Infinity DAD compare to 1200 Series DAD SL
1260/1290 DAD60 mm
1200 DAD SL 10mm
Height [mAU] 28.876 4.938
Noise [mAU] 0.0098 0.0190
Signal/ noise 2944 259
Increase 11.4 x
Columns: 150 x 4.6mm Zorbax SB C18, 5µmSample: Anthracene: 835 pg/µLMobile phase: A: Water, B: Acetonitrile Elution: isocratic 80 % BInjection volume: 5 µLFlow: 1.5 mL/minDAD: 251/4nm, Ref= 450/80nm,
2.5Hz, slit width 4nm
min0 1 2 3 4 5
mAU
0
5
10
15
20
25
30
1200 Series DAD / DAD SL
1260/1290 Infinity DAD
Column Options
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Option PhaseID
(mm)Length (mm)
Particle(µm)
Part Number
#096 EC-C18 3.0 100 2.7 695575-302T#097 EC-C18 3.0 150 2.7 693575-302T#098 EC C18 2.1 50 1.9 699675-902T
#093 EC-C18 4.6 100 2.7 695975-902T#094 EC-C18 3.0 150 2.7 693975-302T#095 EC C18 3.0 50 2.7 699975-302T
#090 EC-C18 4.6 100 4 695970-902T#091 EC-C18 4.6 50 2.7 699975-902T#092 EC-C18 4.6 150 4 693970-902T
Isocratic (G7110B)Quaternary (G7111B)Binary (G7112B)
Quaternary VL (G7111A)
400
bar
Every pump is shipped with a packed column (one pre-written tag included).One column must be chosen (no multiple choices)!
600
bar
Flexible (G7104C)
80
0 b
ar
1000 bar
1000 bar
NEW
Perfect fit for your specific solutions
Scaling Performance – 1260 Quat for UHPLC Performance in EVERY LAB!
Resolution optimized3.0x100mm, 1.8µmResolution peak 5 = 7.16Run time 7 min0.9mL/min, 460bar
Speed and resolution optimized3.0x50mm, 1.8µmResolution (peak 5) = 4.79Run time 1.1min2.4ml/min, 540bar
Conventional4.6x150, 5µmResolution peak 5 = 4.20Run time 11 min
min0 2 4 6 8 10
min0 2 4 6 8 10
min0 2 4 6 8 10
10x productivity increase and improved data quality!
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Agilent Infinity II
Feature
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Vision Scientific
2019
Feature – Injector Program
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• Dengan injector program, user dapat menggunakan sample preparasididalam alat, dan juga untuk analisa asam amino menggunakan proses derivatisasi secara automatisasi dapat memberikan hasil yang optimal dan menghilangkan error yang dapat terjadi saat preparasi
Feature – Leak sensor• Leak Sensor
• Leak Sensor di setiap module (pump, autosampler, column compartment, detector), darifungsi leak sensor ini akansegera menghentikaninstrument yang sedangberjalan dan juga akanmenginformasikan telah terjadikebocoran kepada user
• Bottle Fillings
• Fitur software dimana kondisipump akan off jika solvent habis, sehingga akanmenghindarkan kerusakanpiston dan seal pada pompa
Prevent analysis if level falls below and Turn pump off if running out of solvent
Lab Advisor Basic Highlights
Tests
Calibrations
Tools
Firmware Update
Logs and Results
Fast Connect
System Overview
System Report
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Dengan fitur ini, user akan lebihdimudahkan dalam menanganitroubleshooting sebagaipenanganan awal dalam masalahyang dihadapi
Advanced EMF
Lab Advisor BasicKeep Track of Instrument Wear
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Fine tune EMF values to reflect your use of theinstument and get an alert well before instrument efficiencydegrades.
InfinityLab LC CompanionOverview
A modern replacement of the Agilent Instant Pilot (G4208A) for LCs as a general local remotecontroller is required. The new product is a firmware (FW) web-service, providing remote control capability of PL29 instrumentation on platform independent client hardware (HW)
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A new state-of-the-art local user interface
Agilent Dissolution
Product Portofolio
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Vision Scientific
2019
Dissolution Product PortfolioOverview
• Dosage Forms We Test
• USPApparatus 1, 2, 5, 6 & Intrinsic
• Dissolution Accessories
• Qualification Instrumentation
• USP Apparatus 3 and 7
• Small Volume Dissolution
• On Line Analysis
• Dissolution Workstation Software
Dissolution ProductPortfolioWhat do we test?
• Dissolution is not just about orally ingestedproducts.
• We test tablets, capsules, powders, ointments, creams, gels, osmotic pumps, transdermal patches, implants, medicated contact lens, stents, coated beads, catheters, wound care products, etc.
Drug Eluting StentContact LensPacemaker Lead Ophthalmic Implant
Tablets/capsules
Suspensions
Ointments
Transdermal patches
Dissolution Product PortfolioDissolution Apparatus
Depending on the nature of the dosage form, the USPrecommends using specific apparatus for testing:
USP Dissolution Apparatus 1, 2, 5, 6:1 – Rotating Basket2 – Paddle5 – Paddle over Disk 6 – Rotating Cylinder
USP Dissolution Apparatus 3, 7:3 – Reciprocating Cylinder7 – Reciprocating Holder
Dissolution Product Portfolio708-DS Dissolution Apparatus – for USP Apparatus 1/2/5/6
708-DS Dissolution Apparatus
Dissolution Product Portfolio708-DS Dissolution Apparatus – for USP Apparatus 1/2/5/6
Key Features:
Flexibility – 1-Liter standard configuration; can also support 100/200 mL or 2-Liter volumes
Automated – add sampling, temperature, or dosage introduction to improve repeatability
Scalable – add key features at any time to improve testing at any level
Manual
Semi-Automated
Online
Dissolution Product PortfolioAccessories for 708-DS (USP Apparatus 1, 2, 5, 6)
USP 1: Rotating Baskets USP 2: Paddles
USP 5: Paddle over Disk (Transdermal patches)
USP 6: Rotating Cylinder (Transdermal patches)
Dissolution Product PortfolioOther Accessories for 708-DS
Enhancer Cells <1724> (Gels, creams, ointments) Intrinsic Dissolution
Sinkers Stationary Basket“Mini” Accessories
Dissolution Product PortfolioDissolution Qualification Tool(s)
280-DS MechanicalQualification System (MQS)
• RPM• Shaft and Basket Rim Wobble• Shaft and Vessel Verticality• Vessel Table Level• Vibration• Temperature• Vessel Centering• Paddle/Basket Depth
Dissolution Product PortfolioDissolution Qualification Tool(s)
280-DS MechanicalQualification System (MQS)
• RPM• Shaft and Basket Rim Wobble• Shaft and Vessel Verticality• Vessel Table Level• Vibration• Temperature• Vessel Centering• Paddle/Basket Depth
Dissolution Product Portfolio280-DS Mechanical Qualification System (280-DS)
280 DS MQS benefits:• Time Savings (30 minutes vs 2 days)
• Data that helps identify instrument problems
• Easily track and monitor variations over time
• No guesswork or manually documenting values
• No standards or tablets to purchase
• Daily tool for failure investigations
• Ease of use allows for shorter calibration intervals –narrows the scope of potential failure investigations
Dissolution Product PortfolioDissolution Apparatus 3, 7
BIO-DIS Reciprocating Cylinder (Apparatus 3) (1L, 300ml,100ml & 50ml)
Apparatus 7 Reciprocating Holder (300ml,100ml & 50ml)
Dissolution Product Portfolio400-DS – Small Volume USP Apparatus 7
• Reciprocal movement (2 cm)
• 5 or 10 mL “closed” sample cells
• External jacket design to precisely control the internal temperature
• Visibility to the sample and holderwithin the cell
• Integrated sampling – includes built-in syringe pump and individual rows for sample collection
• Up to 13 samples per test
Dissolution Product Portfolio400 DS – Small Volume USP Apparatus 7
Ideal for Medical Devices or
“Combination Products”:
• Drug-coated stents / balloons
• Pacemaker leads
• Catheters
• Transdermals
• Contact lenses
• Implants
• Other novel dosage forms
Dissolution Product Portfolio850-DS Dissolution Sampling Station
• Integrated syringe pump and fraction collector – saves bench space!
• Streamlined design for ease of use and reliable, repeatable operation
• One sampler per dissolution apparatus for ultimate flexibility
• Built-in options and components for all types of dissolution methods
Dissolution Product Portfolio850-DS Features and Options
Standard:• Media replacement
• Automated cleaning
Optional:• Built-in printer• Filter module (for 0.45 or 0.2
µm filtration)
• Sample Trays
Dissolution Product Portfolio850-DS System Configurations
708-DS Dissolution Apparatus
Cary 60 UV-Vis Spectrophotometer
850-DS Sampling Station (with Filter Module)
Dissolution Workstation Software
Bio-Dis Reciprocating Holder orApparatus 7 Reciprocating Cylinder
Dissolution Product Portfolio 850-DS Features and Options
Confidentiality LabelJuly 13, 201818
Agilent Dissolution PortfolioDrug Development
Software: Dissolution Workflow Software (21 CFR Part 11 Compliance) Win UVUV ChemStationComing soon: Colligo (2019)
Qualification
280-DS
708-DS Bio-Dis
Coming soon…
Cary 8454
Cary 60 Multicell & Fiber Optic
Apparatus 7
25% Manual I
400-DSSmall volume dissolution
with autosampling
75% Automated *
Manual SemiAutomated
850-DSAutomated Sampling Station
Online
+ +
November 15, 2018
Dissolution Product PortfolioDissolution Workstation Software
Dissolution Workstation Software (DWS)
Flexible – up to 4 systems at any time.(DS708+850)x 4
Compliant – designed for 21 CFR Part 11 compliance
Efficient – paperless solution reduces manualtranscription
Secure – integrated data security with easy export features
Use Agilent’s 280-DS instrument module with DWS to monitor vibration data throughout the dissolution test and to aid in potential failure investigations!
Dissolution Product Portfolio“Sample Prep” to complement analytical finish
The Agilent Advantage
Agilent is the only dissolution manufacturer that can also provide the necessary analytical solution.
Variety of scalable systems availabledepending on customer’s needs:
•Manual: 708-DS
•Semi-automated: 708-DS + 850-DS
•Online UV Dissolution: Cary 60 or 8454
Capitalize on the strength provided by local support, training, years of expertise (VanKel > Varian > Agilent) and data management solutions.
Why Agilent?
1. Give a single Solution, from instrument, consumables and method (application note) for your analysis
2. The instrument is Robust and reliable
3. Vision Teknik give full support for Technical and Application issue
4. Low cost of ownership
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InfinityLab Columns and Supplies
• InfinityLab Quick-Connect and Quick-Turn Fittings
• InfinityLab Stay Safe Caps and Solvent Bottles
• InfinityLab FlexBench
• InfinityLab Lamps with RFID Tags
• InfinityLab Valves, Flow Cells and Pump Heads with RFID Tags
• InfinityLab Quick-Connect Heat Exchangers
• InfinityLab Poroshell Columns
• InfinityLab Column identification tags
• InfinityLab Instrument kits
• InfinityLab Maintenance kits
• InfinityLab Capillaries, Pistons, Syringes and Loops
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New and existing InfinityLab Supplies used in and around the InfinityLab LC Series giving Analytical-, Instrument- and Lab- Efficiency enhancements by Ease of Use, Safety and lowest Cost of Ownership
Full InfinityLab LC Portfolio including Bio-inert and Preparative LC
InfinityLab Columns and Supplies
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Portfolio Reminder
InfinityLab Flex Bench
InfinityLab Fittings, Parts & Consumables
InfinityLab Poroshell Columns
New InfinityLab Supplies
1260 Infinity II Prime LC
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New InfinityLab Anti-Drain Fitting• Provides easy control of solvent drainage
• Routine maintenance without spilling solvent
• No introduction of unwanted air into the system.
New InfinityLab Benchtop
Most easiest module exchange!
InfinityLab Benchtop and InfinityLab Anti-Drain Fittings
Questions and Discussion
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