Post on 09-Aug-2020
FURTHER RECENT READING FROM CELL REPORTS
R A S S I G N A L I N G A N D C A N C E R
Dadi Jiang, Edward L. LaGory, Daniela Kenzelmann Brož, Kathryn T. Bieging, Colleen A. Brady, Nichole Link, John M. Abrams, Amato J. Giaccia, and Laura D. Attardi (2015). Analysis of p53 Transactivation Domain Mutants Reveals Acad11 as a Metabolic Target Important for p53 Pro-Survival Function. Cell Rep. 10, 1096–1109
Edward C. Stites, Paul C. Trampont, Lisa B. Haney, Scott F. Walk, Kodi S. Ravichandran (2015) Cooperation between Noncanonical Ras Network Mutations. Cell Rep. 10, 840
Bo-Kuan Wu, Charles Brenner (2014) Suppression of TET1-Dependent DNA Demethylation Is Essential for KRAS-Mediated Transformation. Cell Rep. 9, 1827–1840
Simona Lamba, Mariangela Russo, Chong Sun, Luca Lazzari, Carlotta Cancelliere, Wipawadee Grernrum, Cor Lieftink, Rene Bernards, Federica Di Nicolantonio, Alberto Bardelli (2014) RAF Suppression Synergizes with MEK Inhibition in KRAS Mutant Cancer Cells. Cell Rep. 8, 1475–1483
W N T /β - C AT E N I N S I G N A L I N G A N D C A N C E R
Victoria Llado, Yuki Nakanishi, Angeles Duran, Miguel Reina-Campos, Phillip M. Shelton, Juan F. Linares, Tomoko Yajima, Alex Campos, Pedro Aza-Blanc, Michael Leitges, Maria T Diaz-Meco, Jorge Moscat (2015)Re-pression of Intestinal Stem Cell Function and Tumorigenesis through Direct Phosphorylation of β-Catenin and Yap by PKCζ. Cell Rep. 10, 740–754
Ekaterina Posokhova, Animesh Shukla, Steven Seaman, Suresh Volate, Mary Beth Hilton, Bofan Wu, Holly Morris, Deborah A. Swing, Ming Zhou, Enrique Zudaire, Jeffrey S. Rubin, Brad St. Croix (2015) GPR124 Functions as a WNT7-Specific Coactivator of Canonical β-Catenin Signaling. Cell Rep. 10, 123–130
Hadrien Demagny, Tatsuya Araki, Edward M. De Robertis (2014) The Tumor Suppressor Smad4/DPC4 Is Regulated by Phosphorylations that Integrate FGF, Wnt, and TGF-β Signaling. Cell Rep. 9, 688–700
Je-Hoon Song, David J. Huels, Rachel A. Ridgway, Owen J. Sansom, Boris N. Kholodenko, Walter Kolch, Kwang-Hyun Cho (2014) The APC Network Regulates the Removal of Mutated Cells from Colonic Crypts. Cell Rep. 7, 94–103
m T O R I N C A N C E R
Xin Zhou, Terri L. Clister, Pamela R. Lowry, Marcus M. Seldin, G. William Wong, Jin Zhang (2015) Dynamic Visualization of mTORC1 Activity in Living Cells. Cell Rep. 10, 1767–1777
Shigeru Kawabata, José R. Mercado-Matos, M. Christine Hollander, Danielle Donahue, Willie Wilson, Lucia Regales, Mohit Butaney, William Pao, Kwok-Kin Wong, Pasi A. Jänne, Phillip A. Dennis (2014)Rapamycin Prevents the Development and Progression of Mutant Epidermal Growth Factor Receptor Lung Tumors with the Acquired Resistance Mutation T790M. Cell Rep. 7, 1824–1832
Valentina Pirazzoli, Caroline Nebhan, Xiaoling Song, Anna Wurtz, Zenta Walther, Guoping Cai, Zhongming Zhao, Peilin Jia, Elisa de Stanchina, Erik M. Shapiro, Molly Gale, Ruonan Yin, Leora Horn, David P. Carbone, Philip J. Stephens, Vincent Miller, Scott Gettinger, William Pao, Katerina Politi (2014) Acquired Resistance of EGFR-Mutant Lung Adenocarcinomas to Afatinib plus Cetuximab Is Associated with Activation of mTORC1 Cell Rep. 7, 999–1008
Filip Janku, David S. Hong, Siqing Fu, Sarina A. Piha-Paul, Aung Naing, Gerald S. Falchook, Apostolia M. Tsimberidou, Vanda M. Stepanek, Stacy L. Moulder, J. Jack Lee, Rajyalakshmi Luthra, Ralph G. Zinner, Russell R. Broaddus, Jennifer J. Wheler, Razelle Kurzrock (2014) Assessing PIK3CA and PTEN in Early-Phase Trials with PI3K/AKT/mTOR Inhibitors Cell Rep. 6, 377–387
www.cell.com/cell-reports/collections/pathways
CyclinA1
SurvivinCyclinD1Telomerase
Akt
GlycogensynthesisGlycogensynthesis
G1/S cell-cycleprogression
G1/S cell-cycleprogression
ChromatinremodelingChromatinremodeling
SurvivalSurvival
AminoacidsAminoacids
RapamycinRapamycin
Insulin/IGFGrowth factorInsulin/IGFGrowth factor
Growth factore.g., EGFGrowth factore.g., EGF
AutophagyAutophagy
Cell survivalApoptosis
Cell survivalApoptosis
MetabolismMetabolism
Inactive complexInactive complex
Non-TCF β-cat targetsNon-TCF β-cat targets TCF/LEF target genesTCF/LEF target genes
HypoxiaHypoxiaRibosome biogenesis
TranslationRibosome biogenesis
Translation
Bad
Mdm2
Msk Rsk2 Ets1
Fos Jun
MnSODBimGadd45p27FasL
CyclinD1Fos
Spry
MycCyclinD1
p27
p21
RasRasGDP GTP
Raf
Mek
KSR
SosGrb2
Gab1
SrfElk1
FoxO
NF1 p120GAP
Myc
Erk RSK
Adipose-specific genes
p21CIP1, FASPTEN, TSC2
rRNA
Fas-L, p27KIP1p27
p16
p53
Rb
Cyclin D1CDK4
PPARγ
mTORC1PML
Crm1
TIF-1APol I
UBFSL1
eEF2KS6PDCD4
elF4AelF4E eEF2
elF3
elF4B
LKB1
AMPK
AMPK
AKT
PDK1PTEN
PI3K
PI3K
PIP3PIP2
S6K
4E-BP
TSC1
RagARagC
Rheb
PP2A
PP2A
PP2A
RhebTSC2
REDD1/2
PRAS40
GDP
GTP
BADFOXO3a
FOXO3a
mTORC1 mTORC2
AKT
Receptor TyrosineKinase
Receptor Tyrosine Kinase, EGFR
FKBP
IRS-1 GRB2
14-3-3
Brm
PAF1
MEDCBP
β-catLgsPygo
Pitx2/Prop1
DvI
PR55
GSK3CKI
CKIγ
β-arr
p68
GBP/Frat
AxinCav
FrizzledLRP5/6
Wnt
Axin CKIMACF1
G
TCF
β-catGSK3
GSK3
β-cat
Thermo Scientific™ Pierce™ Antibodies are developed for a wide variety of application needs. We offer more than 40,000 antibodies in 50 research areas, including cancer, epigenetics, and stem cell research. All of our antibodies are validated and guaranteed to perform in the stated application and species. Also, our Innovator’s Program rewards researchers for sharing data on qualified antibodies.To learn more, go to lifetechnologies.com/antibodies
Illustrated are some of the known components of three signaling pathways that have been linked to cancer: Ras, mTOR, and Wnt/β-catenin. Although these are not the only signaling pathways linked to cancer, and the chart is not comprehensive, they serve to illustrate the connectedness of these pathways and to provide an entryway into exploring functional studies, as outlined by the tables provided by Thermo Fisher Scienti�c.
Brown, E.J., Albers, M.W., Shin, T.B., Ichikawa, K., Keith, C.T., Lane, W.S., and Schreiber, S.L. (1994). A mammalian protein targeted by G1-arresting rapamycin-receptor complex. Nature 369, 756–758.
Cafferkey, R., Young, P.R., McLaughlin, M.M., Bergsma, D.J., Koltin, Y., Sathe, G.M., Faucette, L., Eng, W.K., Johnson, R.K., and Livi, G.P. (1993). Dominant missense mutations in a novel yeast protein related to mammalian phosphatidylinositol 3-kinase and VPS34 abrogate rapamycin cytotoxicity. Mol. Cell. Biol. 13, 6012–6023.
Clevers. H., and Nusse, R. (2012). Wnt/β-Catenin Signaling and Disease. Cell 149, 1192-1205
Cully M., and Downward, J. (2008). Snapshot: Ras Signaling. Cell 133, 1292
MacDonald, B.T., Semenov N.V., and He, X. (2007). Snapshot: Wnt/β-Catenin Signaling Cell 131, 1204
Nishisho, I., Nakamura, Y., Miyoshi, Y., Miki, Y., Ando, H., Horii, A., Koyama, K., Utsunomiya, J., Baba, S., and Hedge, P. (1991). Mutations of chromosome 5q21 genes in FAP and colorectal cancer patients. Science 253, 665–669
Nusse, R., and Varmus, H.E. (1982). Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell 31, 99–109
Kinzler, K.W., and Vogelstein, B. (1996). Lessons from hereditary colorectal cancer. Cell 87, 159–170
Kunz, J., Henriquez, R., Schneider, U., Deuter-Reinhard, M., Movva, N.R., and Hall, M.N. (1993). Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression. Cell 73, 585–596
Laplante, M. and Sabatini, D.M. (2012) mTOR signaling in growth control and disease. Cell 149, 274-293
Prior, I.A. et al. (2012) A comprehensive survey of Ras mutations in cancer. Cancer Res. 72, 2457–2467
Sabatini, D.M., Erdjument-Bromage, H., Lui, M., Tempst, P., and Snyder, S.H. (1994). RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 78, 35–43.
Sabers, C.J., Martin, M.M., Brunn, G.J., Williams, J.M., Dumont, F.J., Wiederrecht, G., and Abraham, R.T. (1995). Isolation of a protein target of the FKBP12-rapamycin complex in mammalian cells. J. Biol. Chem. 270, 815–822
Schmick, M., Kraemer, A., Bastiaens, P.I.H. (2015) Ras moves to stay in place. Trends in Cell Biol. http://dx.doi.org/10.1016/j.tcb.2015.02.004
Soulard A. and Hall, M.N. (2007) Snapshot: mTOR signaling. Cell 129, 434
The above touches only the surface of the rich information available about these pathways and the many unknowns that remain. Some further reading, as well as recent insights into these pathways and cancer that have been published in Cell Reports, can be found on the left. Points of potential path-way crosstalk are highlighted in red in the diagrams above.
R E F E R E N C E S
Signaling Pathways in Cancer
β-catGSK3
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
β-cat
APCPR61
AxinWTX
PP1
CKI
Target List Rank GeneID Target Host Reactivity Applications Catalog Number
ERK/RAS/RAF/MEK 1 801 Calmodulin Mouse Ba, Bv, Ck, Hu, Ms, Rt
WB, IF, ICC, IHC, FACS, ELISA MA3-917
ERK/RAS/RAF/MEK 2 1956 EGFR Mouse Hu, Ms WB, IF, ICC, IHC (P), FACS, IP MA5-13070
ERK/RAS/RAF/MEK 3 3480 IGF-IR / IGF1 Receptor alpha Mouse Hu IF, ICC, IHC (P), IP, ELISA MA5-13799
ERK/RAS/RAF/MEK 4 598 BCL-XL Mouse Hu, Ms IF, ICC, IHC (P), FACS, IP MA5-11950
ERK/RAS/RAF/MEK 5 25 c-Abl Mouse Hu, Ms WB, IF, ICC, IHC (P), IP MA5-14398
ERK/RAS/RAF/MEK 6 207 AKT Rabbit Hu, Ms WB, IF, ICC, IHC (P), FACS 710005
ERK/RAS/RAF/MEK 7 2902 NMDA Receptor 1 Rabbit Hu, Ms, Rt WB, IF, ICC, IHC, IP, ELISA PA3-102
ERK/RAS/RAF/MEK 8 2904 NMDA Receptor 2B Rabbit Hu, Ms, Rt WB, IF, ICC, IHC, IP,
ELISA PA3-104
ERK/RAS/RAF/MEK 9 3643 INSR / Insulin Receptor alpha Mouse Bv, Hu, Ov, Po,
Rb, RtIF, ICC, IHC (P), FACS, ELISA MA5-13767
ERK/RAS/RAF/MEK 10 3815 c-Kit / CD117 Rabbit Ca, Hu, Rt WB, IF, ICC, IHC (P), FACS PA5-16770
ERK/RAS/RAF/MEK 11 5921 GTPase Activating Protein Mouse Bv, Hu, Ms, Nhp, Rt WB, IF, IP MA4-001
ERK/RAS/RAF/MEK 12 7422 VEGF Mouse Hu WB, IHC (P) MA5-12184
ERK/RAS/RAF/MEK 13 4804 NGFR / p75 NGF Receptor Mouse Fe, Ft, Hu, Nhp,
Rb, Rt WB, IF, ICC, FACS MA5-13311
ERK/RAS/RAF/MEK 14 208 AKT2 Mouse Hu, Ms, Nhp, Rt WB, IF, ICC, IHC, IP, ChIP MA1-034
ERK/RAS/RAF/MEK 15 998 cdc42 Rabbit Hu, Ms, Nhp, Rt WB, IF, IHC (P) PA1-092
ERK/RAS/RAF/MEK 16 2002 ELK1 Mouse Bv, Hu, Ms, Nhp, Rt WB, IF, IP 45-8700
ERK/RAS/RAF/MEK 17 2260 FGFR1 / CD331 Mouse Hu, Rt WB, IHC, IP, ELISA 37-9200
ERK/RAS/RAF/MEK 18 2324 Flt-4 Rabbit Hu, Ms, Rt WB, IHC (P), IP PA5-16871
ERK/RAS/RAF/MEK 19 3082 Hepatocyte Growth Factor Mouse Hu, Ms WB, IF, ICC MA5-14160
ERK/RAS/RAF/MEK 20 3265 v-H-Ras Rat Hu, Ms, Rt WB, IF, IHC, IP 33-7200
ERK/RAS/RAF/MEK 21 4233 c-Met Mouse Hu WB, IHC, IP, ELISA 37-0100
ERK/RAS/RAF/MEK 22 5156 PDGF-RA / CD140a Rabbit Hu WB, IF, ICC, ChIP 701142
ERK/RAS/RAF/MEK 23 5159 PDGF-RB / CD140b Rabbit Hu, Ms, Rt WB, IF, ICC, IHC (F), IHC
(P), IP MA5-15143
ERK/RAS/RAF/MEK 24 5594 p42 MAP Kinase / ERK2 / MAPK1 Mouse Ca, Hu, Ms, Nhp, Rt WB, IF, IHC (P), IP, ChIP MA1-099
ERK/RAS/RAF/MEK 25 5595 p44 MAP Kinase / ERK1 Mouse Hu, Ms, Nhp WB, IF, IHC, ChIP MA1-13041
ERK/RAS/RAF/MEK 26 5879 Rac1 Rabbit Hu, Ms WB, IF, IHC (P) PA1-091
ERK/RAS/RAF/MEK 27 7535 Phospho-Zap-70 pTyr315+319 Rabbit Hu WB, IF, ICC, FACS 700177
ERK/RAS/RAF/MEK 28 1942 Ephrin-A1 Rabbit Hu, Ms WB, IHC, IP 34-3300
ERK/RAS/RAF/MEK 29 1945 Ephrin-A4 Rabbit Hu, Ms WB, IHC, IP 34-3700
ERK/RAS/RAF/MEK 30 2263 FGFR2 / CD332 Rabbit Hu, Ms, Nhp, Rt WB, IF, IHC, FACS PA5-14651
ERK/RAS/RAF/MEK 31 3630 Insulin Mouse Hu IHC (P) MA5-12029
ERK/RAS/RAF/MEK 32 5578 PKC alpha Mouse Bv, Ca, Hu, Ms, Nhp, Rb, Rt WB, IF, IHC (P), IP MA1-157
ERK/RAS/RAF/MEK 33 5906 Rap1 Mouse Hu, Ms WB, IF, ICC, IHC (P), IP MA1-013
ERK/RAS/RAF/MEK 34 382 Arf6 Rabbit Hu, Ms, Nhp WB, IF, IHC (P) PA1-093
ERK/RAS/RAF/MEK 35 1950 EGF Mouse Hu WB, IF, ELISA M806
ERK/RAS/RAF/MEK 36 2113 ETS1 Mouse Hu, Ms WB, IF, ICC, IHC, FACS MA5-15609
ERK/RAS/RAF/MEK 37 2247 FGF-2 Rabbit Hu WB 710148
ERK/RAS/RAF/MEK 38 2261 FGFR3 / CD333 Rabbit Hu WB, IF, ICC, IHC (P), IP MA5-14843
ERK/RAS/RAF/MEK 39 2321 Flt-1 Rabbit Hu, Ms, Rt WB PA5-16493
ERK/RAS/RAF/MEK 40 2903NMDA Recep-tor Subunit 2A / NMDAR2A
Rabbit Hu, Ms, Rt WB, IHC A-6473
Ras is at the core of the cell signaling network in response to growth factors, and mutations that lead to its activation are strongly linked to cancer, being found in 20–30% of tumors. A small membrane-lo-calized GTPase, Ras, cycles between a GTP-bound active state, which is promoted by guanine nucle-otide exchange factors, and a GDP-bound form. The GTPase activity is stimulated by GTPase-activat-ing proteins. The Ras pathway signals through a phosphorylation cascade that runs through Raf/ME-K/ERK, as highlighted here.
RASThe Wnt signaling pathway has been tied to tumorigenesis since the discovery of the �rst Wnt family member as the site of MMTV integration in a mouse breast tumor model. As further components of the pathway were uncovered, mutations in a downstream component of the pathway, APC, were linked to familial adenomatous polyposis. Many intriguing questions surrounding this pathway (a core version of which is shown here) remain, regarding the mechanisms involved in modi�cation of Wnt family mem-bers, as well as the receptor and co-receptor families (Frizzled and LRPs) and the regulation of a key downstream component, β-catenin.
WNTInitially uncovered as genetic and biochemical targets of rapamycin, mTOR1 and mTOR2 form complexes that respond to a range of environmental and nutritional triggers. Stimulated by the Ras-like protein Rheb, as well as the kinases Akt/PKB, ERK1/2, and RSK1, the mTOR complexes lie at the heart of a network of signaling pathways that are wired into both Ras/ERK and Wnt pathway components. Some examples of the affected pathways, which include links to protein synthesis and energy consumption, are illustrated. These central roles in cellular proliferation and metabolism indicate why mutations in mTOR signaling components (such as TSC1/2) have been linked to familial cancer and why mTORC1 itself, as well as downstream components, can be dysregulated in cancer.
mTOR
Target List Rank GeneID Target Host Reactivity Applications Catalog Number
mTOR-PI3K 1 596 BCL-2 Mouse Hu, Ms WB, IF, ICC, IHC (P), FACS, IP MA5-11757
mTOR-PI3K 2 898 Cyclin E Mouse Hu, Ms WB, IF, IHC (P), FACS MA5-14336
mTOR-PI3K 3 1956 EGFR Mouse Hu, Ms WB, IF, ICC, IHC (P), FACS, IP MA5-13070
mTOR-PI3K 4 3091 HIF-1 alpha Mouse Bv, Hu, Ms, Nhp, Po WB, IF, ICC, IHC (P), IP, GS, IE MA1-516
mTOR-PI3K 5 3320 HSP90 / Heat Shock Protein 90 alpha Mouse Ck, Fs, Hu, Ms,
Nhp, Rt WB, IF, IHC, IP MA3-011
mTOR-PI3K 6 4609 c-Myc Mouse Hu WB, IF, ICC, IHC (P, F), FACS, IP, ChIP, ELISA MA1-980
mTOR-PI3K 7 7057 Thrombospondin Mouse Bv, Ca, Eq, Hu, Ms, Ov, Po, Rt
WB, IF, ICC, IHC (P), FACS, IP, IM MA5-13398
mTOR-PI3K 8 7157 p53 Mouse Bv, Hu WB, IF, ICC, IHC (P), FACS, IP MA5-12557
mTOR-PI3K 9 7184 GRP94 / HSP90B1 Rat Bv, Ck, Hm, Hu, Ms, Rt WB, IF, ICC, IHC (P), IP MA3-016
mTOR-PI3K 10 11140 Cdc37 Mouse Hm, Hu, Ms, Rt WB, IF, ICC, IHC (P), FACS, IP MA3-029
mTOR-PI3K 11 595 Cyclin D1 / Bcl-1 Rabbit Hu, Ms WB, IF, ICC, IHC (P) MA5-14512
mTOR-PI3K 12 896 Cyclin D3 Mouse Hu, Ms, Nhp, Rt WB, IF, IHC (P), FACS, IP MA5-12717
mTOR-PI3K 13 1019 Cdk4 Mouse Hu, Ms, Rt WB, IF, FACS, IP MA5-13498
mTOR-PI3K 14 1021 Cdk6 Mouse Hu, Ms, Rt WB, IF, ICC, IHC (F), IHC (P), IP MA5-13333
mTOR-PI3K 15 1027 p27Kip1 Mouse Ca, Hu, Ms, Rt WB, IF, FACS, IP MA5-12835
mTOR-PI3K 16 1129 Muscarinic Acetylcholine Receptor M2 Mouse Hu, Ms, Po, Rt WB, IF, IHC (P), FACS, IP MA3-044
mTOR-PI3K 17 1280 Collagen II Mouse Bv, Ck, Hu, Ms, Rt WB, IF, IHC (P), FACS MA1-37493
mTOR-PI3K 18 3480 IGF-IR / IGF1 Receptor alpha Mouse Hu IF, ICC, IHC (P), IP, ELISA MA5-13799
mTOR-PI3K 19 598 BCL-XL Mouse Hu, Ms IF, ICC, IHC (P), FACS, IP MA5-11950
mTOR-PI3K 20 3676 Integrin alpha 4 / CD49d Mouse Hu, Rt IHC, FACS, Neu MA49D7
mTOR-PI3K 21 5618 Prolactin Receptor Mouse Eq, Hu, Po, Rb, Rt WB, IF, ICC, IHC (F), IHC (P), FACS, IP MA1-610
mTOR-PI3K 22 207 AKT Rabbit Hu, Ms WB, IF, ICC, IHC (P), FACS 710005
mTOR-PI3K 23 894 Cyclin D2 Mouse Hu, Ms, Rt WB, IF, FACS, IP MA5-12712
mTOR-PI3K 24 1385 CREB Mouse Hu, Ms, Nhp, Rt WB, IF, IHC, IP, ELISA MA1-083
mTOR-PI3K 25 2335 Fibronectin Mouse Hu, Ms, Rt WB, IF, IHC (P) MA5-11981
mTOR-PI3K 26 3559 IL-2R alpha / CD25 Mouse Hu WB, IF, ICC, IHC, FACS, IP M10AR
mTOR-PI3K 27 3643 INSR / Insulin Receptor alpha Mouse Bv, Hu, Ov, Po,
Rb, RtIF, ICC, IHC (P), FACS, ELISA MA5-13767
mTOR-PI3K 28 3815 c-Kit / CD117 Rabbit Ca, Hu, Rt WB, IF, ICC, IHC (P), FACS PA5-16770
mTOR-PI3K 29 5728 PTEN Mouse Hu WB, IF, ICC, IHC (P) MA5-12278
mTOR-PI3K 30 6198 Phospho-p70 S6 Kinase pThr389 Rabbit Hu, Rt WB, IF, ICC, FACS, ChIP 710095
mTOR-PI3K 31 7422 VEGF Mouse Hu WB, IHC (P) MA5-12184
mTOR-PI3K 32 3672 Integrin alpha-1 / CD49a Mouse Hu WB, IHC (F), FACS, IP MA49A0
mTOR-PI3K 33 3688 Integrin beta-1 / CD29 Mouse Hu FACS MA2910
mTOR-PI3K 34 4804 NGFR / p75 NGF Re-ceptor Mouse Fe, Ft, Hu, Nhp,
Rb, Rt WB, IF, ICC, FACS MA5-13311
mTOR-PI3K 35 4846 eNOS Rabbit Bv, Ca, Hu, Ms, Rt WB, IF, ICC, IHC, IHC (P) PA3-031A
mTOR-PI3K 36 208 AKT2 Mouse Hu, Ms, Nhp, Rt WB, IF, ICC, IHC, IP, ChIP MA1-034
mTOR-PI3K 37 842 Caspase 9 Rabbit Bv, Hu, Ms, Ov, Rt WB, IHC (P), IP PA5-16358
mTOR-PI3K 38 1017 Cdk2 Mouse Hu, Ms, Rt WB, IF, IHC (P) MA5-13479
mTOR-PI3K 39 1026 p21 Waf1/Cip1 Rabbit Ca, Hu, Nhp WB, IF, ICC, IHC (P), FACS, IP MA5-14949
mTOR-PI3K 40 1977 eIF4E Mouse Hu, Ms WB, IF, ICC, IP MA1-089
Target List Rank GeneID Target Host Reactivity Applications Catalog Number
WNT/ β-Catenin 1 1387 CREB Binding Protein Rabbit Hu, Ms WB, IF, ICC, IHC (P),
ChIP PA1-847
WNT/ β-Catenin 2 4609 c-Myc Mouse HuWB, IF, ICC, IHC (P, F), FACS, IP, ChIP, ELISA
MA1-980
WNT/ β-Catenin 3 4772 NFATc1 Mouse Hu, Ms, Nhp, RtWB, IF, ICC, IHC, IHC (P), IHC (PFA), IP, ChIP, GS
MA3-024
WNT/ β-Catenin 4 4773 NFATc2 Mouse Hu, Ms, Rt WB, IF, ICC, IHC (P, F), IP, ChIP, GS MA1-025
WNT/ β-Catenin 5 4776 NFAT3 Rabbit Hu WB, IF, IHC, IP PA1-021
WNT/ β-Catenin 6 7157 p53 Mouse Bv, Hu WB, IF, ICC, IHC (P), FACS, IP MA5-12557
WNT/ β-Catenin 7 595 Cyclin D1 / Bcl-1 Rabbit Hu, Ms WB, IF, ICC, IHC (P) MA5-14512
WNT/ β-Catenin 8 896 Cyclin D3 Mouse Hu, Ms, Nhp, Rt WB, IF, IHC (P), FACS, IP MA5-12717
WNT/ β-Catenin 9 5663 Presenilin 1 Mouse Hu, Ms, Nhp, Rt WB, IF, ICC, IHC, ELISA MA1-752
WNT/ β-Catenin 10 815 Phospho-CaM Kinase II pThr286 Mouse Hu, Ms, Rt WB, IF, ICC, IHC,
FACS, IP, ELISA MA1-047
WNT/ β-Catenin 11 894 Cyclin D2 Mouse Hu, Ms, Rt WB, IF, FACS, IP MA5-12712
WNT/ β-Catenin 12 1499 beta Catenin Mouse Hu, Ms WB, IF, IP MA1-2001
WNT/ β-Catenin 13 5467 PPAR delta Rabbit Hu, Ms, Rt WB, IF, IHC (F) PA1-823A
WNT/ β-Catenin 14 816 CaM Kinase II beta Mouse Fs, Hu, Ms, Ov, Rt, Su WB, IHC, IP, ELISA 13-9800
WNT/ β-Catenin 15 2033 EP300 Mouse Hu WB, IF, IHC, IP 33-7600
WNT/ β-Catenin 16 4316 MMP-7 (Matrilysin) Mouse Hu IF MA5-14215
WNT/ β-Catenin 17 5879 Rac1 Rabbit Hu, Ms WB, IF, IHC (P) PA1-091
WNT/ β-Catenin 18 6885 TAK1 / MAP3K7 Rabbit Hu WB, IF, ICC, FACS 700113
WNT/ β-Catenin 19 8312 Axin Rabbit Hu WB, IF, IP 34-5900
WNT/ β-Catenin 20 5578 PKC alpha Mouse Bv, Ca, Hu, Ms, Nhp, Rb, Rt WB, IF, IHC (P), IP MA1-157
WNT/ β-Catenin 21 2535 Frizzled-2 / FZD2 Rabbit Hu WB, IP 38-4700
WNT/ β-Catenin 22 2932 GSK3 Rabbit Hu WB, IF, ICC 701102
WNT/ β-Catenin 23 3725 c-Jun Mouse Hu, Ms, Nhp WB, IF, ICC, IHC, FACS MA5-15881
WNT/ β-Catenin 24 4041 LRP-5 Rabbit Hu, Ms WB, IF 36-5400
WNT/ β-Catenin 25 4088 SMAD3 Rabbit Hu WB, IF, IHC, ELISA 51-1500
WNT/ β-Catenin 26 4089 Smad4 Rabbit Hu, Ms, Nhp, Rt WB, IF, ChIP PA5-16191
WNT/β-Catenin 27 6932 TCF1 Rabbit Hu, Ms WB, IF, ICC, IHC (P), FACS, IP MA5-14965
WNT/β-Catenin 28 7471 WNT1 Mouse Hu, Ms WB, IF, ICC, IHC, FACS MA5-15544
WNT/β-Catenin 29 8945 BTRC Mouse Hu WB, IP, ELISA 37-3400
WNT/β-Catenin 30 51176 LEF1 Rabbit Hu, Ms, Rt WB, IF, ICC, FACS MA5-14966
WNT/β-Catenin 31 1460 Casein Kinase 2 beta Rabbit Hu, Ms WB, IF, ICC, IHC (P), ChIP PA5-27416
WNT/β-Catenin 32 5582 PKC-gamma Mouse Hu, Ms, Rt WB, IHC 13-3800
WNT/β-Catenin 33 8454 CUL-1 Rabbit Hu WB, IP, ELISA 71-8700
WNT/β-Catenin 34 9978 ROC1C Rabbit Hu, Ms WB, IP 34-2500
WNT/β-Catenin 35 817 CaMKII delta Rabbit Hu, Ms, Rb, Rt WB, IF, ICC PA5-22168
WNT/β-Catenin 36 5566 PKA C-alpha Rabbit Hu, Ms, Rt WB, IF, ICC, FACS, IP PA5-17626
WNT/β-Catenin 37 5599 JNK1 Rabbit Hu, Ms, Rt WB 44-690G
WNT/β-Catenin 38 5881 Rac1, 2, 3 Rabbit Bv, Hu, Ms, Nhp, Rt, Xl WB, FACS PA5-17519
WNT/β-Catenin 39 6934 TCF4 Rabbit Hu, Ms, Rt WB, IP, ChIP MA5-14975
WNT/β-Catenin 40 7473 WNT3 Mouse Hu, Ms, Rt WB, ELISA 39-0300
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AACR_final.pdf 1 4/2/15 10:40 AM