3M products for Food safety solution

© 3M 2009. All Rights Reserved.

Le Thi Cam Tu – Technical Service Support

15 – 16 Aug 2012

1) Indicator Organism Testing => Petrifilm

2) Pathogen & Toxin Testing => Tecra kit (Eli sa), MDS (DNA )

3) Rapid Hygiene Monitoring => ATP NG Machine, Pro-tec

PRODUCT CATALOGUE

Group Typical products

4) End product screening => MLS II Machin e

5) Sample Handling & Media => Broth, Sponge

PETRIFILM TECRA - ELISA MDS - DNA Detection

PRODUCT CATALOGUEPlease visit our website :http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety

3

3M3MTMTM PetrifilmPetrifilmTMTM PlatesPlates

Indicator Organism Testing

Only 3 steps :

Incubate CountInoculate

Indicator Organism Testing

Direct Plating 1ml :

Water testing

5

Plating with Filter :

1 hr

Indicator Organism Testing

EnterobacteriaceaeYeast & Mould Rapid Coliform

ColiformsAerobic

Indicator Organism Testing

7

E. coli & coliformsSelect E. coliStaph Express Evironment Listeria

High sensitive Coliforms

Indicator Organism Testing

http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-information/regulatory/

Regulatory information

8

Indicator Organism Testing

http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-information/regulatory/

Regulatory information

9

Indicator Organism Testing

10

11

1) AC (Aerobic count)

� All bacteria growth with presence of

Oxygen.

� 48h

� Count red colonies.

� AOAC 986.33; 989.10; 990.12

Indicator Organism Testing

� AOAC 986.33; 989.10; 990.12

2) CC (Coliforms count)

� VRB (Violet Red Bile agar)

� 24h

� Count red colonies with gaz

� AOAC 986.33; 989.10; 2000.15

3) EC/CC (E.coli / Coliforms count)

� 24h – 48h

� Red colonies + gas : coliforms

� Red-blue colonies + gas : E.coli

� AOAC 991.14 ; 998.08

Indicator Organism Testing

4) SEC (Selective E.coli)

� 24h

� Blue colonies

Indicator Organism Testing

5) EL (Environment Listeria)� 31h

� Red - violet colonies

� AOAC PTM

6) STX (Staphylococcus express)

14

6) STX (Staphylococcus express)

� 24h

� Purple colonies

� AOAC 2003.07; 2003.08; 2003.11*** In case there are green/black colonies, insert

additional disk and incubate 3h more and read colonies

with pink zone as positive colonies.

Indicator Organism Testing

7) RCC (Rapid Coliforms count)� 6h – 14h

� Red colonies with gas

� AOAC 2000.15*** Early appear yellow acid zone at 6h

15

8) High sensitive Coliforms

� 5ml sample => sensitivity 2 cfu /g

� 24h

� Red colonies with gas

� AOAC 996.02

Indicator Organism Testing

9) EB (Enterobacteriaceae) � 24h

� Red colonies with gas or with yellow

acid zone or with both.

� AOAC 2003.01

16

10) YM (Yeast and Mold)

� 3 – 5 days

� Yeast : small colonies with defined

edge, uniform color (blue/green).

� Mold : large colonies with diffused

edge, dark center, varied in color.

� AOAC 997.02

Indicator Organism Testing

3M™ Petrifilm™ Plate

Applied for Environmental Monitoring

1) Air Sampling Method

Hydrate plate with 1ml sterile diluent,

let gel solidified

17

2) Direct contact Method

3) Swab Method

Incubate

Indicator Organism Testing

Storage condition :

• Before opened : < / = 8oC until 18 months after manufacturing date.

• After opened : seal pouch with tape, store at room temperature and use within 1 month.

18

use within 1 month.

Indicator Organism Testing

Summary testing procedure by Petrifilm vs tradition al agar:

19

Indicator Organism Testing

Summary FAB (Features, Advantages, Benefits) of Pet rifilm :

20

Indicator Organism Testing

Source for documents of Petrifilm :http://solutions.3m.com/wps/portal/3M/en_US/Microbi ology/FoodSafety/product-applications/one/

21

For more information, please kindly contact :

Msc. Le Thi Cam Tu – Technical support3M Food Safety Division

22

3M Food Safety Division3M Vietnam Ltd,77 Hoang Van Thai – Tan Phu ward – Dist 7 – HCM City

Email : tu.legandon@mmm.com

Tel : 09 33 64 01 18

23