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3D Bioprinting of VesselsA new means to Improve patient Outcomes

John Geibel,MD,DSc,MSc,AGAFVice Chairman Department of SurgeryDirector of Surgical ResearchProfessor of Surgery and Cellular and Molecular PhysiologyAmerican Gastroenterological Association Fellow

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3D Bioprinting of Vessels, a new means to Improve patient Outcomes

• Disclosures– Organovo

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Three-Dimensional (3D) Printing

• Also known as additive manufacturing, refers to any of several processes involving sequential deposition of material to form a 3D objecthttps://en.wikipedia.org/wiki/3D_printing

https://chemical-materials.elsevier.com/the-world-of-3d-printing-in-2015/

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3D Bioprinting of Vessels, a new means to Improve patient Outcomes

• Are we ready for 3D printing in the 21st Century ?

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•Yes

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Bioprinting

• How Does it Work ?• Use Printer with 2 Print Cylinders

– Cells– Hydrogel

• Create 3D Model of Vessel

• Prepare Vessel for Implantation

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Schematic of the Process

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Creation of Rat Arterial Vessel: Elapsed time to Finished Print 3 Hours

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Cross-Section of Print 36+ hours from Print

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Perfusion of New Print Technology

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Question of Tensile Strength

• Are the new prints able to withstand the pressures of aortic perfusion?

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Rat Aorta

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Implanted full length graft

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Intestinal Repair with 3D Prints

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Larger ID multilayer robust print

• Print at 24 hours post print

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Process For Intestinal Patch

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In Vivo 21 days Post Op

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30 Days Post Implantation of Patch

Implant Cross Section Sham Operated Cross Section

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Yale Instrumentation Initiative Help

• Critical Needs for your Instrumentation Work– Supplies to expand cell culture levels to move on to higher organisms– Acquisition of More incubators and TC hood to allow for more prints

• How could the Yale Instrumentation Initiative help your work

– Provide infrastructure to make facility available to more labs– Expand the printing facility to make it accessible to other labs/groups

• What can you offer to the Yale Instrumentation Community

– Consult on what is needed for successful 3D Bioprinting– Potentially print organoids for drug development using human cell

models– Work on designing Cartridges for 3D Organ replacement

• Pancreas• Liver• Kidney

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Acknowledgements

• Muyoka Maina(Yale)• Peter Geibel (Yale)• Brian Wengerter (Yale)• Gulus Emre (Yale)• Alan Dardik (Yale)• Hualong Bai (Yale)• Taras Lysyy (Yale)• Julian Campillo Luna(Yale)• Michael Shang(Yale)• Jack Gagne(Yale)• Organovo technical support team

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Thank you!

• Questions?