Post on 19-Feb-2018
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4.3 (a) To investigatethe efect o pH onamylase activity
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Before you start get ormake a copy of this
Table o results
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Step 1
Set up a water bath at 37 C.
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Step 2
Place 5 ml starch solution and 3 drops of
iodine solution into 3 boiling tubes.
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Step 3
Put 2 ml amylase solution and 5 ml
buffer (! 7! "# into three other tubes.
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Step 4
Place all tubes into a water bath
at 37 C and lea$e for 5 minutes.
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Step 5
Pour one of the amylase tubes into
one of the starch tubes. %ently
sha&e. 'epeat for each p.
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Step 6
'eplace the tubes in the water bath and
measure the time ta&en for the
blue)blac& colour to disappear.
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%raph your results
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Step 7
*est for sugar+ add ,ml -enedicts
solution to each boiling tube and
heat for 5 minutes.
Prepare as for
steps 1 and 2
previously
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Step 8
/ bric& red colour indicates a
reducing sugar is present.
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Step 9
'epeat the whole e0periment
with boiled amylase as a control.
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10pected result
The enzyme works best
at a pH of 7.
The blue / black colour
faded and enedicts
resulted in a positivetest.
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Results
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END