Post on 08-Nov-2018
Curriculum Vitæ ‐ Liliana Bernardino
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1. PERSONAL INFORMATION Name: Liliana Inácio Bernardino Date of birth: 07/11/1980 Birth place: Trancoso, Guarda Nacionality: Portuguese Civil Status: Married Institutional address Personal address Center for Neuroscience and Cell Biology Rua Tenente Campos Rego, nº 31, 2º C. Department of Zoology 3000‐339 Coimbra University of Coimbra 3004‐517 Coimbra Mobile Phone: 965361114 Fax (CNC): 239853409 Email: libernardino@gmail.com
2. ACADEMIC DEGREES 2008: PhD in Molecular Biology, University of Coimbra, Coimbra, Portugal.
2003: BSc. in Biology (17/20, Very good), Faculty of Science and Technology (FCTUC), University of Coimbra, Coimbra, Portugal. 3. PREVIOUS AND CURRENT SCIENTIFIC AND/OR PROFESSIONAL ACTIVITIES
• March 2008 ‐ Present Postdoctoral fellow at the Center for Neuroscience and Cell Biology, University of Coimbra, Portugal. This work has been performed under the supervision of Dr. João Malva and Dr. Fabienne Agasse and supported by a fellowship from the Portuguese Foundation for Science and Technology (FCT), SFRH/BPD/34841/2007.
• October 2003 – February 2008 PhD fellow at the Center for Neuroscience and Cell Biology, University of Coimbra. This work was performed under the supervision of Dr. João Malva and Dr. Emília Duarte and was supported by a fellowship from the Portuguese Foundation for Science and Technology (FCT), SFRH/BD/12731/2003.
• November 2007 – February 2008 Research grant (BI) attributed by the FCT
• May 2004 ‐ March 2005 PhD fellow at the Institute for Pharmacological Research “Mario Negri”, Department of Neuroscience, Milan, Italy, under the supervision of Dr. Annamaria Vezzani.
• October 2003 ‐ December 2003 / February 2004 ‐ April 2004 PhD fellow at the Department of Anatomy and Neurobiology, University of Southern Denmark, Odense, Denmark, under the supervision of Dr. Jens Zimmer.
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• September 2002 ‐ July 2003 Undergraduate training at the Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal, under the supervision of Dr. Emília Duarte, with the project “Effects of oxidative stress on GDNF expression and release in postnatal cell cultures from substantia nigra.“ Participation in the PRODEP III project supported by the Portuguese Foundation for Science and Technology. 4. PUBLICATIONS 4.1. PHD THESIS
Bernardino L (2008) Inflammation in neuronal death and survival: Involvement of Tumor Necrosis Factor‐alpha and Interleukin‐1 beta. 4.2. PAPERS IN INTERNATIONAL SCIENTIFIC PERIODICALS WITH REFEREES
2010
12. Bernardino L, Eiriz MF, Xapelli S, Grade S, Rosa A, Cortes C, Agasse F, Ferreira L and Malva JO (2010) Histamine is a proneurogenic factor in neural stem cell cultures from the subventricular zone. In preparation for submission to the PNAS Journal.
11. Maia J, Santos T, Aday S, Agasse F, Cortes L, Xapelli S, Malva JO, Bernardino L*, Ferreira L* (2010) Controlling the neuronal differentiation of stem cells by the intracellular delivery of retinoic acid‐loaded nanoparticles. ACS Nano Journal; Accepted for publication with minor revisions. *These authors shared senior authorship. Impact Factor (2009): 7.493
10. Rosa AI, Gonçalves J, Cortes L, Bernardino L, Malva JO, Agasse F (2010) The angiogenic factor angiopoietin‐1 is a proneurogenic peptide on subventricular zone stem/progenitor cells. J Neurosci. 30(13): 4573‐4584. Impact Factor (2009): 7,178
9. Grade S, Agasse F, Bernardino L, Silva CG, Cortes L, Malva (2010) Functional identification of neural stem cell‐derived oligodendrocytes by means of calcium transients elicited by thrombin. Rejuvenation Research. 13(1): 27‐37. Impact Factor (2009): 4,138
2008
8. Bernardino L, Agasse F, Silva BA, Ferreira R, Grade S, Malva JO (2008) Tumor Necrosis Factor‐alpha modulates survival, proliferation and neuronal differentiation in neonatal subventricular zone cell cultures. Stem Cells 26(9): 2361‐2371. Impact Factor (2009): 7,747
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7. Agasse F*, Bernardino L*, Silva BA, Ferreira R, Grade S, Malva JO (2008) Response to histamine allows the functional identification of neuronal progenitors, neurons, astrocytes and immature cells in subventricular zone cell cultures. Rejuvenation Res 11(1): 187‐200. *Both authors contributed equally. Impact Factor (2009): 4,138
6. Bernardino L, Balosso S, Ravizza T, Marchi N, Ku G, Randle JC, Malva JO, Vezzani A (2008) Transient inflammatory‐like events in hippocampal slice cultures prime neuronal susceptibility to excitotoxic injury: a crucial role of P2X7 receptor‐mediated IL‐1beta release. J Neurochem. 106(1): 271‐280. Impact Factor (2009): 3,999
5. Agasse F, Bernardino L, Kristiansen H, Christiansen SH, Ferreira R, Silva BA, Grade S, Woldbye DPD, Malva JO (2008) Neuropeptide Y promotes neurogenesis in murine Subventricular Zone. Stem Cells 26(6): 1636‐1645. Impact Factor (2009): 7,747
4. Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2008) Interaction between NPY and BDNF in NPY‐mediated neuroprotection against excitotoxicity: a role for microglia. Eur J Neurosci 27(8): 2089‐2102. Impact Factor (2009): 3,418
2006
3. Ravizza T, Lucas S‐M, Balosso S, Bernardino L, Ku G, Noe’ F, Malva JO, Randle JC, Allan S, Vezzani A (2006) Inactivation of caspase‐1 in rodent brain: a novel anticonvulsive strategy. Epilepsia 47(7): 1160‐1168. Impact Factor (2009): 4,052
2005
2. Bernardino L, Ferreira R, Cristovão AJC, Sales F, Malva JO (2005) Inflammation and Neurogenesis in Temporal Lobe Epilepsy. Curr Drug Targets CNS Neurol Disord 4(4): 349‐460. Impact Factor (2009): 3,932
1. Bernardino L, Xapelli S, Silva AP, Jakobsen B, Poulsen FR, Oliveira CR, Vezzani A, Malva JO, Zimmer J (2005) Modulator effects of Interleukin‐1 beta and Tumor Necrosis Factor‐alpha on AMPA‐induced excitotoxicity in mouse organotypic hippocampal slice cultures. J Neurosci 25(29): 6734‐6744. Impact Factor (2009): 7,178
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4.3. CHAPTERS IN BOOKS
2010
4. Eiriz MF, Grade S, Rosa A, Xapelli S, Bernardino L, Agasse F, Malva JO (2010) Functional evaluation of neural stem cell differentiation by single cell calcium imaging. Editor: António Salgado Current Stem Cell Research and Therapy. In press.
2008
3. Bernardino L*, Agasse F*, Malva JO (2008) Neurogenesis and Inflammation. Marc D Binder, N Hirokawa, Martin C Hirsch, U Windhorst (Editors), pp. 2676‐2680. Encyclopedia of Neuroscience, Springer‐Verlag, GmbH Berlin Heidelberg *Both authors contributed equally.
2007
2. Bernardino L, Malva JO (2007) Inflammation and neuronal susceptibility to excitotoxic cell death. In: Interaction between neurons and glia in aging and disease, pp. 3‐36; João O Malva, Ana C Rego, Rodrigo A Cunha, Catarina R Oliveira (Editors) Springe‐Verlag, New York.
1. Agasse F, Bernardino L, Malva JO (2007) SVZ cells as a tool for brain repair. In: Interaction between neurons and glia in aging and disease, pp. 81‐108; João O Malva, Ana C Rego, Rodrigo A Cunha, Catarina R Oliveira (Editors) Springe‐Verlag, New York. 4.4. PATENTS 4. “Controlling the neuronal differentiation of stem cells by the intracellular delivery of retinoic acid‐loaded nanoparticles” Inventors: Maia J, Santos T, Malva JO, Bernardino L, Ferreira L (Reference: PAT 20101000042173/0198)
3. “Histamine released from biocompatible microparticles induce neurogenesis in the murine subventricular zone” Inventors: Bernardino L, Agasse F, Eiriz MF, Ferreira L, Malva JO (Reference: PAT 20091000098745/0198) 2. “Functional identification of SVZ‐derived oligodendrocytes based on selective response to thrombin”; Inventors: Grade S, Malva JO, Bernardino L, Agasse F (Reference: PAT 2008100089504/0198)
1. “Calcium Imaging as a tool to functionally evaluate neuronal differentiation in stem/progenitor cell cultures: screening of neurogenic factors useful for, but not restricted to, brain repair and improvement”; Inventors: Agasse F, Bernardino L, Silva BA, Malva JO (Reference: W02008100168‐A1)
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4.5. PAPERS IN CONFERENCE PROCEEDINGS • FIRST AUTHOR
2010
13. Bernardino L, Eiriz MF, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira F, Malva JO (2010) Histamine promotes neurogenesis in subventricular zone cell cultures: a new approach for brain repair therapies. FENS Abstr., vol.5, 011.6. Poster presented at the “7th FENS Forum of European Neuroscience”, Amsterdam, The Netherlands, 3‐7 July.
2009
12. Bernardino L, Eiriz MF, Xapelli S, Grade S, Cortes L, Agasse F, Ferreira F, Malva JO (2009)
Histamine released from biocompatible microparticles induce neurogenesis in the murine subventricular zone. Soc. Neurosci. Abstr.,30.15. Poster presented at the “39th Annual Meeting of the Society for Neuroscience”, Chicago, EUA, 17‐21 October.
11. Bernardino L, Xapelli S, Grade S, Rosa A, Agasse F, Malva JO (2009) Histamine induces neuronal differentiation in murine subventricular zone (SVZ) cell cultures. Frontiers in Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.051. Poster presented at the “11th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Braga, 4‐6 June.
2008
10. Bernardino L, Agasse F, Silva B, Ferreira R, Grade S, Malva JO (2008) Interplay between neurogenesis and neuroinflammation: Tumor Necrosis factor‐alpha as a key modulator of SVZ neurogenesis in vitro. Soc. Neurosci. Abstr., 522.6. Poster presented at the “38th Annual Meeting of the Society for Neuroscience”, Washington, EUA, 15‐19 November. 9. Bernardino L, Agasse F, Silva BA, Ferreira R, Grade S, Malva JO (2008) Modulator effects of Tumor Necrosis Factor‐alpha on Subventricular Zone neurogenesis. FENS Abstr., vol. 4, A009.2. Poster presented at the “6th FENS Forum of European Neuroscience”, Genève, Switzerland, 12‐16 July.
2007
8. Bernardino L, Agasse F, Ferreira R, Grade S, Silva BA, Malva JO (2007) Tumor Necrosis Factor‐alpha promotes neuronal differentiation from Subventricular Zone cell cultures. Soc. Neurosci. Abstr., 673.17. Poster presented at the “37th Annual Meeting of the Society for Neuroscience”, San Diego, EUA, 3‐7 November.
7. Bernardino L, Agasse F, Silva BA, Grade S, Ferreira R, Malva JO (2007) Role of TNF‐alpha in neurogenesis: new potential tool for brain repair? Neuron Glia Biology, vol. 2, suppl. 1, S79, A148. Poster presented at the “VIII European Meeting on Glial Cell in Health and Disease”, London, UK, 4‐8 September.
6. Bernardino L, Agasse F, Grade S, Ferreira R, Silva BA, Malva JO (2007) Tumor necrosis factor‐alpha modulates cell survival, proliferation and neuronal differentiation in neonatal neural stem cells. J. Neurochem. vol. 102, pag. 293, suppl. 1. Abstract submitted to the “21st Biennial Meeting
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of the International Society for Neurochemistry / 38th Annual Meeting of the American Society for Neurochemistry”, Cancun, Mexico, 19‐24 August.
2006
5. Bernardino L, Agasse F, Grade S, Silva AP, Ferreira R, Silva BA, Malva JO (2006) Tumor necrosis factor‐alpha induces differentiation of new neurons in neonatal subventricular zone cell cultures. Soc. Neurosci. Abstr., 221.8. Poster presented at the “36th Annual Meeting of the Society for Neuroscience”, Atlanta, EUA, 14‐18 October.
2005
4. Bernardino L, Balosso S, Marchi N, Ku G, Randle JCR, Zimmer J, Malva JO, Dinarello C and Vezzani A (2005) Inflammatory stimuli in organotypic hippocampal slice cultures induces microglia‐like cell apoptosis via P2X7 receptors: relevance for neuronal survival to excitotoxicity. Soc. Neurosci. Abstr., 906.6. Poster presented at the “35th Annual Meeting of the Society for Neuroscience”, Washington DC, EUA, 12‐16 November.
3. Bernardino L, Rizzi M, Marchi N, Ravizza T, Ku G, Randle JCR, Zimmer J, Malva JO, Vezzani A (2005) Adenosine Triphosphate induces microglia‐like cell death in Lipopolysaccharide‐primed mouse organotypic hippocampal slice cultures via Tumour Necrosis Factor‐alpha release. J. Neurochem., vol. 94, suppl. 2, pag. 180. Poster presented at the “20th Biennial Meeting of the International Society for Neurochemistry jointly with the European Society for Neurochemistry”, Innsbruck, Austria, 22‐26 August.
2. Bernardino L, Xapelli S, Silva AP, Poulsen FR, Jakobsen B, Oliveira CR, Vezzani A, Malva JO, Zimmer J (2005) Neuroprotective and neurotoxic effects mediated by activation of distinct Tumor Necrosis Factor (TNF)‐α receptor subtypes in mouse organotypic hippocampal slice cultures. ‘Sinapse’, vol. 5, YL1, nº1, pag. 96, Maio de 2005. Oral communication in the “9th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Luso, 19 and 20 May.
2004
1. Bernardino L, Xapelli S, Poulsen FR, Jakobsen B, Zimmer J, Duarte EP and Malva JO (2004) Effect of TNF‐alpha and Interleukin‐1beta in AMPA‐mediated neuronal death in mouse organotypic hippocampal slice cultures. FENS Abstr. vol. 2, A045.05. Poster presented at the”4th FENS Forum of European Neuroscience”, Lisboa, 10‐14 July. • CO‐AUTHOR
2010
26. Xapelli S, Bernardino L, Cortes L, Schitine CS, Melo Reis RA, Agasse F, Malva JO (2010) Type 1 cannabinoid receptor (CB1R) activation promotes neuronal differentiation in murine subventricular zone cell cultures. FENS Abstr., vol.5, 073.38. Poster presented at the “7th FENS Forum of European Neuroscience”, Amsterdam, The Netherlands, 3‐7 July. 25. Baptista S, Bento AR, Gonçalves J, Bernardino L, Fontes Ribeiro C, Malva JO, Agasse F, Silva AP (2010) Neuropeptide Y promotes proliferation, neuronal differentiation and protection against methamphetamine‐induced toxicity in dentate gyrus cultures. FENS Abstr., vol.5, 041.2. Poster
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presented at the “7th FENS Forum of European Neuroscience”, Amsterdam, The Netherlands, 3‐7 July.
2009
24. Agasse F, Christiansen SH, Rosa A, Schitine C, Bernardino L, Malva JO, Woldbye DPD (2009) The neuropeptide Galanin promotes neuronal differentiation in murine subventricular zone cultures. Soc. Neurosci. Abstr., 30.5. Poster presented at the “39th Annual Meeting of the Society for Neuroscience”, Chicago, EUA, 17‐21 October.
23. Grade S, Agasse F, Bernardino L, Silva CG, Cortes L, Malva JO (2009) Thrombin‐induced intracellular calcium rise identifies oligodendrocytes derived from Subventricular Zone cell cultures. Soc. Neurosci. Abstr., 126.4. Poster presented at the “39th Annual Meeting of the Society for Neuroscience”, Chicago, EUA, 17‐21 October.
22. Grade S, Agasse F, Bernardino L, Gomes C, Malva JO (2009) Thrombin‐induced intracellular calcium rise identifies oligodendrocytes derived from SVZ cultures. Frontiers in Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.014. Oral communication in the “11th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Braga, 4‐6 June.
21. Agasse F, Christiansen SH, Rosa A, Schitine C, Bernardino L, Malva JO, Woldbye DPD (2009) Neuropeptides galanin and somatostatin promote neuronal differentiation in murine subventricular zone cultures. Frontiers in Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.099. Oral communication in the “11th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Braga, 4‐6 June.
20. Baptista S, Bento AR, Bernardino L, Ribeiro CF, Malva JO, Agasse F, Silva AP (2009) Methamphetamine is toxic for murine dentate progenitor cell cultures. Frontiers in Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.048. Oral communication in the “11th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Braga, 4‐6 June.
19. Bento AR, Baptista S, Bernardino L, Ribeiro CF, Malva JO, Silva AP, Agasse F (2009) Methamphetamine exerts toxic effects in murine subventricular zone cell cultures. Frontiers in Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.050. Oral communication in the “11th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Braga, 4‐6 June.
2008
18. Agasse F, Bernardino L, Kristiansen H, Christiansen S, Ferreira R, Silva B, Grade S, Woldbye D, Malva JO (2008) Subventricular zone cells as tool for brain repair. FENS Abstr., vol. 4, A232.1. Oral communication in the “6th FENS Forum of European Neuroscience”, Genève, Switzerland, 12‐16 July.
17. Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2008) Neuroprotective interactions between neuropeptide Y and brain‐derived neurotrophic factor: a role for microglia. FENS Abstr., vol. 4, 115.21. Poster presented at the “6th FENS Forum of European Neuroscience”, Genéve, Switzerland, 12‐16 July.
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2007
16. Agasse F, Bernardino L, Ferreira R, Grade S, Silva BA, Malva JO (2007) NPY promotes neurogenesis in subventricular zone (SVZ) cell cultures. Soc. Neurosci. Abstr., 777.10. Poster presented at the “37th Annual Meeting of the Society for Neuroscience”, San Diego, EUA, 3‐7 November.
15. Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Cavadas C, Grouzmann E, Poulsen F, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2007) Interaction between neurons and microglia in NPY and BDNF‐mediated neuroprotection against excitotoxicity. Soc. Neurosci. Abstr., 352.1. Poster presented at the “37th Annual Meeting of the Society for Neuroscience”, San Diego, EUA, 3‐7 November.
14. Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Cavadas C, Grouzmann E, Poulsen F, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2007) Neuropeptide Y and brain derived neurotrophic factor in neuroprotection against excitotoxicity: A neuron‐microglia crosstalk? J. Neurochem., vol. 102, pag. 229, suppl. 1, 2007. Abstract submitted to the “21st Biennial Meeting of the International Society for Neurochemistry / 38th Annual Meeting of the American Society for Neurochemistry”, Cancun, Mexico, 19‐24 August.
2006
13. Agasse F, Bernardino L, Grade S, Silva AP, Ferreira R, Silva BA, Malva JO (2006) Subventricular zone (SVZ) cells as a tool for neuronal replacement in mice models of temporal lobe epilepsy. Soc. Neurosci. Abstr., 221.8. Poster presented at the “36th Annual Meeting of the Society for Neuroscience”, Atlanta, EUA, 14‐18 October. 12. Malva JO, Bernardino L, Balosso S, Ravizza T, Marchi N, Ku G, Randle JC, Zimmer J, Dinarello C, Vezzani A (2006) Inflammation and interleukin–1beta release exacerbate excitotoxic damage to CA3 pyramidal neurons in mouse organotypic hippocampal slice cultures. FENS Abstr., vol. 3, A202.8, 2006. Poster presented at the “5th FENS Forum of European Neuroscience”, Vienna, Austria, 8‐12 July.
11. Balosso S, Ravizza T, Calcagno E, Mariotto A, Bernardino L, Malva JO, Invernizzi R, Vezzani A (2006) Molecular and functional interactions between TNF‐alpha and glutamate in the mouse hippocampus: Implications for neuronal excitability and seizure susceptibility. Epilepsia, vol. 47, S3, pag. 21. Poster presented at the “7th European Congress on Epileptology”, Helsinki, Filand, 02‐06 July.
2005
10. Xapelli S, Bernardino L, Silva AP, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Zimmer J, Malva JO (2005) Neuroprotection mediated by neuropeptide Y2 receptor activation in mouse hippocampal organotypic cultures: cross‐talk with BDNF. ‘Sinapse’, vol. 5, nº1, pag. 96, Maio de 2005. Oral communication in the “9th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Luso, 19 and 20 May.
9. Vezzani A, Bernardino L, Xapelli S, Silva AP, Poulsen FR, Jakobsen B, Oliveira CR, Malva JO, Zimmer J (2005) Distinct tumor necrosis factor‐alpha receptor subtypes modulate neuronal survival in mouse organotypic hippocampal slice cultures. Soc. Neurosci. Abstr., 906.3. Poster
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presented at the “35th Annual Meeting of the Society for Neuroscience”, Washington DC, EUA, 12‐16 November.
8. Xapelli S, Bernardino L, Silva AP, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Cristóvão AJC, Oliveira CR, Zimmer J and Malva JO (2005) NPY triggers neuroprotection mechanisms following excitotoxic insults in mouse organotypic hippocampal slice cultures. Soc. Neurosci. Abstr., 336.10. Poster presented at the “35th Annual Meeting of the Society for Neuroscience”, Washington DC, EUA, 12‐16 November.
7. Silva AP, Xapelli S, Ferreira R, Bernardino L, Pinheiro PS, Zimmer J, Oliveira CR, Malva JO (2005) Activation of neuropeptide Y1 or Y2 receptors can prevent the excitotoxicity induced by kainate in rat organotypic hippocampal slice cultures. Soc. Neurosci. Abstr., 336.16. Poster presented at the “35th Annual Meeting of the Society for Neuroscience”, Washington DC, EUA, 12‐16 November.
6. Ravizza T, Balosso S, Calcagno E, Rizzi M, Mariotto A, Bernardino L, Malva JO, Invernizzi R and Vezzani A (2005) Molecular and functional interactions between TNF‐alpha and glutamate in the mouse hippocampus: implications for neuronal excitability and cell survival. Soc. Neurosci. Abstr., 906.4. Poster presented at the “35th Annual Meeting of the Society for Neuroscience”, Washington DC, EUA, 12‐16 November.
5. Balosso S, Lucas S‐M, Bernardino L, Malva JO, Ravizza T, Ku G, Randle JCR, Allan S and Vezzani A (2005) Selective inhibition of caspase‐1 reduces interleukin‐1 (IL‐1) beta production and release in rodent brain and protects from kainate‐induced seizures. Epilepsia, vol. 46, S6, pag. 202. Poster presented at the “26th International Epilepsy Congress”, Paris, France, 28 August – 01 September.
4. Ravizza T, Filippi F, Noe’ F, Bernardino L, Balosso S, Ku G, Malva JO, Randle JCR, Vezzani A (2005) Caspase‐1 inhibition protects from seizures by blocking interleukin‐1 (IL‐1) beta production in brain. Epilepsia, vol. 46, S8, pag. 121. Poster presented at the “Joint Annual Meeting of the American Epilepsy Society/American Clinical Neurophsiology Society”, Washington, DC, EUA, 02‐06 December.
2004
3. Silva AP, Xapelli S, Bernardino L, Poulsen FR, Jakobsen B, Oliveira CR, Malva JO and Zimmer J (2004) Neuroprotection and decreased of Brain Derived Neurotrophic Factor expression mediated by neuropeptide Y2 receptor activation in mouse organotypic hippocampal slice cultures. Soc. Neurosci. Abstr., 47.3. Poster presented at the “34th Annual Meeting of the Society for Neuroscience”, San Diego, EUA, 23‐27 October.
2. Malva JO, Bernardino L, Xapelli S, Silva AP, Jackobsen B, Poulsen FR, Oliveira CR and Zimmer J (2004) Activation of microglia in excitotoxic lesions in organotypic slice cultures of the mouse hippocampus. Soc. Neurosci. Abstr., 679.16. Poster presented at the “34th Annual Meeting of the Society for Neuroscience”, San Diego, EUA, 23‐27 October.
2003
1. Saavedra A, Baltazar G, Bernardino L, Carvalho C and Duarte EP (2003) Upregulation of GDNF in substantia nigra postnatal cultures in response to L‐DOPA and hydrogen peroxide. Soc. Neurosci.
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Abstr., 409.21. Poster presented at the “33th Annual Meeting of the Society for Neuroscience”, New Orleans, EUA, 8‐12 November. 5. PARTICIPATION IN RESEARCH PROJECTS 5.1. PRINCIPAL INVESTIGATOR (PI)
1. “Histamine in the neural and cancer stem cell niche: a role in glioblastoma ontogeny?” Funding: Calouste Gulbenkian Foundation Reference: FCG 96542 Duration: January 2009 ‐ December 2011
2. “Histamine versus antihistamines: new modulators of neurogenesis?” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: PTDC/SAU‐NEU/104415/2008 Duration: January 2010 – December 2012 5.2. AS AN INVESTIGATOR OF THE RESEARCH TEAM
1. “Role of intercellular communication between endothelial and stem cells in stemness and neurogenesis: new targets for brain repair therapies.” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: PTDC/SAU‐NEU/101783/2008 January 2010 – December 2012 Principal Investigator: Dr. Fabienne Agasse
2. “Heart regeneration by embryonic vascular cells and a biomimetic scaffold.” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: PTDC/SAU‐BEB/098468/2008 June 2009‐ May 2012 Principal Investigator: Dr. Lino da Silva Ferreira
3. “Subventricular zone stem cells as a tool for brain repair in temporal lobe epilepsy” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: PTDC/SAU‐NEU/68465/2006. January 2007 – December 2009 Principal Investigator: Dr. João José Oliveira Malva
4. “Microglial and neuronal changes in the hippocampus induced by methamphetamine: role of pro‐inflammatory cytokines and neuropeptide Y” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: PTDC/SAU‐FCF/67053/2006. January 2007 – December 2009 Principal Investigator: Dr. Ana Paula Pereira da Silva Martins
5. “Neo‐neurogenesis and microglia in temporal lobe epilepsy: role of pro‐inflammatory cytokines and neuropeptide Y in neuroprotection of new neurons” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: POCTI/SAU‐NEU/58492/2004.
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September 2005 – August 2008 Principal Investigator: Dr. João José Oliveira Malva
6. “Neuroprotective effect of neuropeptide Y in excitotoxicity and in temporal lobe epilepsy” Funding: Portuguese Foundation for Science and Technology (FCT) Reference: POCTI/NSE/46848/2002. September 2003 – August 2006 Principal Investigator: Dr. Catarina Resende de Oliveira 6. PRIZES AND AWARDS • Prize attributed by the Calouste Gulbenkian Foundation ‐ Funding of a research project in the Oncology field (November 2008). 7. PRESENT RESEARCH INTERESTS Excitotoxicity; Neuroprotection; Cell death mechanisms (necrosis/apoptosis); Epilepsy; Neuroinflammation; Neuron‐Glia interaction; Cytokines; Cell Signalling mechanisms; Intracelular calcium regulation; Neurogenesis and Stem cells; Brain Repair therapies; Cancer Stem Cells; Glioblastoma; Cellular and molecular mechanisms of proliferation, self‐renewal and migration of tumoral cells: deregulation of stem cells and genesis of cancer stem cells; Biomaterials as a therapeutic tool for drug delivery at Central Nervous System. 8. TECHNICAL SKILLS • Cell Cultures
‐ Organotypic hippocampal slice cultures (mouse, rat) ‐ Mouse Subventricular zone (SVZ) and Subgranular zone (SGZ) stem/progenitor cell cultures ‐ Primary cell cultures of hippocampal neurons from mouse embryo. ‐ Primary cell cultures of cortical astrocytes from rat ‐ Primary cell cultures of substantia nigra neurons from rat
• Tissue preparations ‐ Acute hippocampal slices from rat/mouse ‐ Celular extracts ‐ Brain sections (coronal, sagital) ‐ Criostat slices from organotypic hippocampal slice cultures
• Stainings ‐ Immunocitochemistry in primary cell cultures ‐ Immunohistochemistry in organotypic hippocampal slices and in total brain slices ‐ Nissl and Toluidine staining
• Microscopy ‐ Transmission ‐ Fluorescence microscopy ‐ Confocal microscopy
• Animal manipulation ‐ Cardiac perfusion (mouse/rat)
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‐ Stereotaxic injection in the lateral ventricles and in brain parenchima (hippocampus, striatum) ‐ Intraperitoneal and oral administration
• Molecular Biology ‐ Western‐blotting ‐ RT‐PCR ‐ DNA and RNA extraction ‐Real Time PCR ‐Chromatin immunoprecipitation (qCHIP)
• Others ‐ Single cell calcium imaging (functional microscopy) ‐ Cell viability analysis: Propidium iodide uptake, Hoechst staining, MTT and TUNEL assay ‐ Proliferation assays: BrdU incorporation ‐ Proteins extraction ‐Cell migration assays: Boyden chambers
9. SUPERVISING EXPERIENCE September 2009 – September 2012 Supervisor (together with Dr. João Malva) of the PhD project of Maria Francisca Santos Eiriz; MIT‐Portugal Doctoral Programme (Bioengineering Systems). Project: “Neurogenesis in the hippocampus: histamine and AMPA receptors facilitate differentiation and synaptic integration of new neurons.”
September 2009 – September 2010 Supervisor (together with Dr. Ana Paula Silva) of the Master project of Sandrine Pontes Machado; Master in Biology, University of Coimbra. Project: “Methamphetamine‐induced microglial cells toxicity: the role of TNF‐alpha”
September 2009 – July 2010 Supervisor (together with Dr. João Malva) of the Master project of Tiago de Sousa Santos; Master in Biochemistry, University of Coimbra. Project: “The role of retinoic acid in SVZ neurogenesis”
January 2009 – July 2009 Supervisor of Sandrine Pontes Machado, in the research project “Seminário II” of the Master in Biology, University of Coimbra. Project: “The neuroprotective effect of neuropeptide Y against Methamphetamine.”
January 2009 – July 2009 Supervisor of Tiago de Sousa Santos, in the research project “Seminário II” of the Master in Biochemistry, University of Coimbra. Project: “Retinoic Acid as a modulator of neurogenesis”
Tutor in lab practicals of the Master in Cell and Molecular Biology (Faculty of Science and Technology, University of Coimbra). January 2009: Lucília Silva February 2009: Hugo Veríssimo Fofo
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2007 ‐ 2008 Collaboration in the supervision of Tiago de Sousa Santos in the laboratory training of the BSc. Course in Biochemistry, University of Coimbra. 10. ACADEMIC DEGREES JURY PARTICIPATION
MASTER DEGREE
2010
• 20 July ‐ Jury of Master in Biochemistry (University of Coimbra). Master Thesis Defense by Tiago de Sousa Santos at the Department of Biochemistry, University of Coimbra.
• 21 July ‐ Principal Jury of Master in Biochemistry (University of Beira Interior – UBI). Master Thesis Defense by Sandra Catarina Moreira Rocha at the Faculty of Health Sciences, University of Beira Interior, Covilhã. • 21 July ‐ Principal Jury of Master in Biochemistry (University of Beira Interior – UBI). Master Thesis Defense by Ana Rita Nunes Parente at the Faculty of Health Sciences, University of Beira Interior, Covilhã.
• 10 September ‐ Jury of Master in Biology (University of Coimbra). Master Thesis Defense by Sandrine Pontes Machado at the Department of Zoology, University of Coimbra. 11. TEACHING ACTIVITIES 11.1. PRE‐GRADUATE
2009
• Teaching activities in the Biomedical Engeneering BSc., University of Coimbra Lesson: “The dual role of neuroinflammation in Brain Repair: Focus on Neuroprotection and Neurogenesis”; 25 November.
2008
• Teaching activities in the Biomedical Engeneering BSc., University of Coimbra Lesson: “Neuroinflammation and Neurogenesis”; 25 November. 11.2. POST‐GRADUATE
2010
• Lecturer at the Doctoral Programme in Health Sciences, Faculty of Medicine, University of Coimbra. Advanced Course: Regenerative Medicine: "The bright and the dark side of Inflammation and Neurogenesis in Epilepsy" Lesson 1: “Neurogenesis and inflammation”; 25 May Lesson 2: “Histamine versus antihistamines: new modulators of neurogenesis?”; 25 May
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• Lecturer at the Doctoral MIT‐Portugal Programme (Bioengineering Systems). Lesson: “Neuroinflammation in Brain Repair”; 27 January.
2009
• Lecturer at the PDBEB (Doctoral Programme in Experimental Biology and Biomedicine), Center for Neuroscience and Cell Biology, University of Coimbra. Advanced Course: “Neurogenesis” Lesson: “Neuroinflammation in neurodegeneration and neurogenesis”; 25 March.
2008
• Lecturer at the PDBEB (Doctoral Programme in Experimental Biology and Biomedicine), Center for Neuroscience and Cell Biology, University of Coimbra. Advanced Course: “Neuroimmunology” Lesson: “Contribution of neuroinflammation to excitotoxicity and neurogenesis”; 2 April.
2006
• Lab Tutorial at the PDBEB (Doctoral Programme in Experimental Biology and Biomedicine), Center for Neuroscience and Cell Biology, University of Coimbra. Advanced Course: “Neuromodulation”. Lesson: ”Organotypic cultures”; 16‐20 January.
2005
• Lab Tutorial in the Master in Cell and Molecular Biology, Center for Neuroscience and Cell Biology, University of Coimbra Lesson: ”Organotypic cultures”; 9‐20 May. 12. COMMUNICATIONS
12.1. ORAL COMMUNICATIONS
• PRESENTED BY LILIANA BERNARDINO
2010
14. Bernardino L, Eiriz MF, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira F and Malva JO (2010) Histamine is a Proneurogenic Factor on Subventricular Zone Stem/Progenitor Cells: A New Approach for Brain Repair Therapies. “ENI‐Net meeting”, Coimbra, 6‐8 May. Invited speaker
2009
13. Bernardino L (2009) As células estaminais neurais na reparação cerebral. “Encontro Nacional dos Estudantes de Bioquímica (ENEBIOQ)”, Covilhã, 28 November. Invited speaker
12. Bernardino L, Eiriz MF, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira F and Malva JO (2009) Histamine released from biocompatible microparticles induce neuronal differentiation in
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the subventricular zone. “XL Annual meeting of the Portuguese Pharmacological Society (SPF)”, Porto, 2‐4 December.
11. Bernardino L (2009) The dual role of neuroinflammation in excitotoxicity and neurogenesis. “1st Portuguese‐Spanish Mini‐Symposium on Glia Cell Biology”, Braga, 4 June. Invited speaker
10. Bernardino L, Agasse F, Xapelli S, Grade S, Rosa A, Malva JO (2009) Histamine promotes neuronal differentiation from subventricular zone (SVZ) cell cultures. “4th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Lisboa, 29‐30 April.
2008
9. Bernardino L, Agasse F, Silva B, Ferreira R, Grade S, Malva JO (2008) Neurogenesis in the Subventricular Zone: Tumor Necrosis Factor‐alpha as a key modulator. “3rd Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Faro, 23‐24 April.
2007
8. Bernardino L, Agasse F, Grade S, Ferreira R, Silva BA, Malva JO (2007) Tumor Necrosis Factor‐alpha triggers differentiation of new neurons in neonatal neural stem cells. “10th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Ofir, 23‐26 May.
7. Bernardino L, Agasse F, Grade S, Ferreira R, Silva BA, Malva JO (2007) Tumor Necrosis Factor‐Alpha modulates cell proliferation, survival and differentiation of new neurons in SVZ cell cultures. “2nd Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Coimbra, 27‐28 April.
2006
6. Bernardino L, Agasse F, Grade S, Ferreira R, Silva BA, Malva JO (2006) Tumor Necrosis Factor‐alpha induces neurogenesis in Subventricular Zone cell cultures. “IV Meeting of the Center for Neuroscience and Cell Biology”, Biocant Park, Cantanhede, 16‐17 December.
5. Bernardino L, Agasse F, Grade S, Ferreira R, Silva BA, Malva JO (2006) Tumor necrosis factor‐alpha induces neurogenesis in subventricular zone cell cultures. “XXXVII Annual meeting of the Portuguese Pharmacological Society (SPF)”, Porto, 5‐7 December.
4. Bernardino L, Agasse F, Grade S, Silva BA, Malva JO (2006) TNF‐alpha induces neuronal differentiation in subventricular zone cell cultures of the mouse brain. “1st Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Funchal, Madeira, 30‐ 31 May.
2005
3. Bernardino L, Xapelli S, Silva AP, Poulsen FR, Jakobsen B, Oliveira CR, Vezzani A, Malva JO, Zimmer J (2005) Neuroprotective and neurotoxic effects mediated by activation of distinct Tumor Necrosis Factor (TNF)‐α receptor subtypes in mouse organotypic hippocampal slice cultures. “9th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Luso, Portugal, 19‐ 20 May.
Invited speaker for the “Young Lecture”
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2. Bernardino L, Balosso S, Ravizza T, Xapelli S, Ku G, Randle JC, Zimmer J, Malva JO, Vezzani A (2005) Activation of P2X7 receptors causes microglia‐like cell apoptosis and contributes to increased susceptibility of pyramidal cells to AMPA‐induced excitotoxicity. “XXXVI Annual meeting of the Portuguese Pharmacological Society (SPF)”, Lisboa, 5‐7 December.
1. Bernardino L, Xapelli S, Silva AP, Poulsen FR, Jakobsen B, Oliveira CR, Vezzani A, Malva JO, Zimmer J (2005) Role of inflammation in neurodegeneration and neuroprotection. “III Meeting of the Center for Neuroscience and Cell Biology”, Bissaya Barreto Institute, Bencanta, 23 September. • PRESENTED BY OTHER CO‐AUTHOR
2010
14. Eiriz MF, Bernardino L, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira L, Malva JO (2010) Histamine releasing PLGA microparticles as a new way to drive brain repair. “5th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Caldas das Taipas, Guimarães, Portugal, 20‐21 May. Prize for the best oral communication
13. Santos T, Maia J, Aday S, Bernardino L, Malva JO, Ferreira L (2010) Nanomaterials to drive the differentiation of stem cells into neuronal cells. “5th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Caldas das Taipas, Guimarães, Portugal, 20‐21 May.
2009
12. Baptista S, Bento AR, Bernardino L, Ribeiro CF, Malva JO, Agasse F, Silva AP (2009) Neuropeptide Y prevents methamphetamine‐induced cell death in Dentate Gyrus cell cultures. “XL Annual meeting of the Portuguese Pharmacological Society (SPF)”, Porto, 2‐4 December.
11. Grade S, Agasse F, Bernardino L, Gomes C, Malva JO (2009) Functional identification of SVZ‐derived oligodendrocytes based on selective response to thrombin. “4th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Lisboa, 29‐30 April.
2008
10. Agasse F, Bernardino L, Kristiansen H, Christiansen S, Ferreira R, Silva B, Grade S, Woldbye D, Malva JO (2008) Subventricular zone cells as tool for brain repair. “6th FENS Forum of European Neuroscience”, Geneve, Switzerland, 12‐16 July.
9. Agasse F, Bernardino L, Silva B, Grade S, Malva JO (2008) Single Cell Calcium Imaging and pharmacology of neurogenesis: a method useful for drug screening in Neural Stem Cell Cultures. “3rd Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Faro, 23‐24 April.
2007
8. Malva JO, Silva AP, Xapelli S, Ferreira R, Bernardino L, Agasse F (2007) Neuropeptide Y in neuroprotection and neurogenesis‐tool for brain repair? “1st Portugal‐Brazil Coloquium in Neurochemistry”, Ofir, 23 May.
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7. Agasse F, Bernardino L, Ferreira R, Grade S, Silva BA, Malva JO (2007) NPY promotes neurogenesis in Subventricular Zone Cell (SVZ) cell cultures. “2nd Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Coimbra, 27‐28 April.
2006
6. Xapelli S, Bernardino L, Silva AP, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2006) Neuroprotection by NPY and BDNF against excitotoxicity in mouse hippocampal slice cultures. “IV Meeting of the Center for Neuroscience and Cell Biology”, Biocant Park, Cantanhede, 16 and 17 December.
5. Xapelli S, Bernardino L, Silva AP, Cavadas C, Grouzmann E, Poulsen F, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2006) Neuroprotection by NPY and BDNF against excitotoxicity in mouse hippocampal slice cultures. “XXXVII Annual meeting of the Portuguese Pharmacological Society (SPF)”, Porto, 5‐7 December.
4. Agasse F, Bernardino L, Grade S, Silva BA, Malva JO (2006) Differentiation of subventricular zone (SVZ) cells following treatment with cytokines: a calcium imaging study. “1st Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)”, Funchal, Madeira, 30 and 31 May.
2005
3. Xapelli S, Bernardino L, Silva AP, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Zimmer J, Malva JO (2005) Neuroprotection mediated by neuropeptide Y2 receptor activation in mouse hippocampal organotypic cultures: cross‐talk with BDNF. “9th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Luso, Portugal, 19 and 20 May.
2004
2. Silva AP, Xapelli S, Pinheiro PS, Bernardino L, Carvalho AP, Carvalho CM, Oliveira CR, Jakobsen B, Zimmer J and Malva JO (2004) Neuroprotection mediated by neuropeptide Y receptors in the hippocampus. “XIV Annual meeting of the Portuguese Society of Biochemistry”, Vilamoura, Portugal, 2‐4 December.
1. Xapelli S, Silva AP, Bernardino L, Jakobsen B, Zimmer J, Oliveira CR and Malva JO (2004) Neuroprotection by Neuropeptide Y receptors activation in excitotoxicity and epilepsy. “4th Forum of European Neuroscience”, Simposium “Brain damage and Repair”, Lisboa, Portugal, 10‐14 July. 12.2. POSTERS IN CONFERENCES • PRESENTED BY LILIANA BERNARDINO
2010
4. Bernardino L, Eiriz MF, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira F, Malva JO (2010) Histamine is a Proneurogenic Factor on Subventricular Zone Stem/Progenitor Cells: A New Approach for Brain Repair Therapies. Congress “ISDN 2010‐ Understanding the basic mechanisms of neurodevelopmental disorders”, Estoril, Portugal, 6‐9 June.
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2006
3. Bernardino L, Balosso S, Ravizza T, Xapelli S, Ku G, Randle JC, Zimmer J, Dinarello C, Malva JO and Vezzani A (2006) Inflammation exacerbate excitotoxic injury in CA3 pyramidal neurons via Interleukin‐1 beta release in organotypic cultures of mouse hippocampal slices. Advanced Course (FENS/PENS Summer School) “The Brain as a target for inflammatory processes”, Berlin, Germany, 14‐20 May.
2005
2. Bernardino L, Balosso S, Ravizza T, Rizzi M, Ku G, Randle JC, Zimmer J, Malva JO and Vezzani A (2005) Involvement of P2X7 receptors in microglia‐like cell death mediated by ATP in LPS‐primed mouse organotypic hippocampal slice cultures. Advanced Course (FENS/Blackwell Summer School) “Neuro‐glia interaction in aging and disease”, Ofir, 5‐10 July.
1. Bernardino L, Balosso S, Rizzi M, Marchi N, Ravizza T, Ku G, Randle JCR, Zimmer J, Malva JO, Vezzani A (2005) Inflammation induced in organotypic hippocampal slice cultures triggers microglia apoptosis mediated by TNF‐alpha release. Congress ”Molecular mechanisms of neurodegeneration”, Milan, Italy, 7‐10 May. • PRESENTED BY OTHER CO‐AUTOR
2010
19. Santos T, Maia J, Aday S, Bernardino L, Malva JO, Ferreira L (2010) Nanomaterials to drive the differentiation of stem cells into neuronal cells. Congress “ESTOOLS‐ International Symposium: Stem Cells in Biology and Disease”, Lisboa, Portugal, 26‐28 May.
18. Xapelli S, Bernardino L, Cortes L, Schitine C, Ferro E, De Melo Reis RA, Agasse F, Malva JO (2010) Type 1 Cannabinoid Receptor Activation Induces Neuronal Differentiation of Subventricular Zone Stem/Progenitor Cell Cultures. Congress “ISDN 2010‐ Understanding the basic mechanisms of neurodevelopmental disorders”, Estoril, Portugal, 6‐9 June. 17. Xapelli S, Bernardino L, Cortes L, Schitine C, Ferro ES, De Melo Reis RA, Agasse F, Malva JO (2010) Activation of type 1 cannabinoid receptor (CB1R) induce neurogensis in the murine subventricular zone. Congress “ESTOOLS‐ International Symposium: Stem Cells in Biology and Disease”, Lisboa, Portugal, 26‐28 May.
16. Eiriz MF, Bernardino L, Xapelli S, Grade S, Rosa A, Cortes L, Agasse F, Ferreira L, Malva JO (2010) Histamine‐releasing PLGA microparticles as a tool to impel brain repair. Congress “ESTOOLS‐ International Symposium: Stem Cells in Biology and Disease”, Lisboa, Portugal, 26‐28 May.
15. Grade S, Agasse F, Bernardino L, Silva CG, Cortes L, Malva JO (2009) L'augmentation du calcium intracellulaire induit par la thrombine est caractéristique des oligodendrocytes provenant de cellules de la zone sous‐ventriculaire. « Journée Scientifique du Centre de recherche Université Laval Robert‐Giffard », Quebéc city, Quebéc, 29 January.
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2009
14. Grade S, Agasse F, Bernardino L, Silva CG, Cortes L, Malva JO (2009) Functional identification of neural stem cell‐derived oligodendrocytes by means of calcium transients elicited by thrombin. “La 3º Journée de la recherché du Regroupement Neurosciences & Santé Mentale de l’Université Laval », Quebéc city, Quebéc, 26 November.
2007
13. Agasse F, Bernardino L, Ferreira R, Grade S, Silva BA, Malva JO (2007) NPY modulates neurogenesis in SVZ cell cultures. “10th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Ofir, Portugal, 23‐26 May.
12. Xapelli S, Bernardino L, Ferreira R, Grade S, Silva AP, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J, Malva JO (2007) Interactions between NPY and BDNF in neuroprotection against excitotoxicity: a role for neuron‐microglia crosstalk? “10th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Ofir, Portugal, 23‐26 May.
2006
11. Xapelli S, Bernardino L, Silva AP, Salgado JR, Cavadas C, Grouzmann E, Poulsen FR, Jakobsen B, Oliveira CR, Zimmer J and Malva JO (2006) Implication of BDNF in the neuroprotective effect of NPY in mouse hippocampal slice cultures exposed to excitotoxic insults. Congress “8th NPY meeting”, St. Petersburg, Florida, EUA, 22‐26 April.
2005
10. Balosso S, Bernardino L, Rizzi M, Calcagno E, Invernizzi R, Zimmer J, Malva JO and Vezzani A (2005) Functional interactions between TNF‐alpha and glutamate in the mouse hippocampus. Advanced course (FENS/Blackwell Summer School) “Neuro‐glia interaction in aging and disease”, Ofir, 5‐10 July.
9. Xapelli S, Silva AP, Ferreira R, Bernardino L, Pinheiro P, Zimmer J, Oliveira CR and Malva JO (2005) NPY Y1 and Y2 receptors activation can rescue neurons following the application of an excitotoxic insult with kainate in rat organotypic hippocampal slice cultures. Advanced course (FENS/Blackwell Summer School) “Neuro‐glia interaction in aging and disease”, Ofir, 5‐10 July.
8. Balosso S, Bernardino L, Ravizza T, Calcagno E, Ravizza T, Rizzi M, Zimmer J, Invernizzi R, Malva JO, Vezzani A (2005) Functional interactions between TNF‐alpha and glutamate in the hippocampus: implications for neuronal excitability and neuronal survival. Congress ”Molecular mechanisms of neurodegeneration”, Milan, Italy, 7‐10 May.
2004
7. Xapelli S, Bernardino L, Silva AP, Poulsen FR, Jakobsen B, Oliveira CR, Malva JO and Zimmer J (2004) Involvement of neuropeptide Y2 receptor in neuroprotection and decrease of brain derived neurotrophic factor expression under excitotoxicity in mouse organotypic hippocampal slice cultures. “XIV Annual meeting of the Portuguese Society of Biochemistry”, Vilamoura, 2‐4 December.
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6. Xapelli S, Bernardino L, Poulsen FR, Jakobsen B, Zimmer J and Malva JO (2004) A activação do receptor Y2 do NPY protege culturas organotípicas de hipocampo de murganho contra agressões excitotóxicas. “IV Congress of Research in Medicine”, Coimbra, 17‐18 November.
5. Xapelli S, Bernardino L, Poulsen FR, Jakobsen B, Zimmer J, Malva JO (2004) Neuropeptide Y2 receptor activation protect mouse organotypic hippocampal slice cultures against excitotoxic insults. Congress “The multiple facets of GABAergic synapses”, La Ciotat, France, 15‐18 September.
4. Xapelli S, Bernardino L, Poulsen FR, Jakobsen B, Zimmer J and Malva JO (2004) Effect of neuropeptide Y2 receptor activation in AMPA‐mediated neuronal death and Brain Derived Neurotrophic Factor expression in mouse organotypic hippocampal slice cultures. FEBS Advanced course “New molecular strategies to treat neurodegenerative diseases”, Ofir, 17‐23 July.
3. Xapelli S, Bernardino L, Silva AP, Jakobsen B, Zimmer J and Malva JO (2004) Activaction of Neuropeptide Y2 and Y5 receptor is neuroprotective against excitotoxicity induced by AMPA in mouse organotypic hippocampal slice cultures. Congress “7th international NPY meeting”, Meliá Hotel, Coimbra, 3‐7 February.
2003
2. Saavedra A, Bernardino L, Baltazar G, Carvalho C and Duarte EP (2003) GDNF expression in the nigrostriatal system: effect of damage to dopaminergic neurons. “8th Annual meeting of the Portuguese Society for Neurosciences (SPN)”, Curia, 12‐13 de Junho.
1. Saavedra A, Bernardino L, Baltazar G and Duarte EP (2003) Oxidative stress and GDNF expression and release in postnatal cultures from substantia nigra. Congress “Frontiers in Neurodegenerative Disorders and Aging: Fundamental Aspects, Clinical Perspectives and New Insights”, Antalya, Turkey, 26 May to 1 June. 13. COMPLEMENTARY ACTIVITIES 13.1. RESEARCH MEETINGS ATTENDED
2010
30. ISDN 2010‐ Understanding the basic mechanisms of neurodevelopmental disorders”, Estoril, 6‐9 June.
29. ESTOOLS‐ International Symposium: Stem Cells in Biology and Disease, Lisboa, 26‐28 May.
28. ENI‐Net Network of European Neuroscience Institutes meeting, Coimbra, 6‐8 May.
27. 7th FENS Forum of European Neuroscience, Amsterdam, The Netherlands, 3‐7 July. ● Fellowship provided by the Calouste Gulbenkian Foundation
2009
26. XL Annual meeting of the Portuguese Pharmacological Society (SPF), Porto, 2‐4 December.
25. 39th Annual Meeting of the Society for Neuroscience, Chicago, EUA, 17‐21 October.
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24. 11th Annual meeting of the Portuguese Society for Neurosciences (SPN), Braga, 4‐6 de Junho.
23. 4th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC), Lisboa, 29‐30 April.
2008
22. VI Annual meeting of the Center for Neuroscience and Cell Biology, BIOCANT, Cantanhede, Portugal, 16‐17 December.
21. 38th Annual Meeting of the Society for Neuroscience, Washington, EUA, 15‐19 November. ● Fellowship provided by the Calouste Gulbenkian Foundation
20. The 8th International Conference of Anticancer Research, Kos Island, Greece, 17‐22 October.
19. 6th FENS Forum of European Neuroscience, Geneve, Switzerland, 12‐16 July. ● Fellowship provided by the Federation of European Neuroscience (FENS)
18. 3th Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC), Faro, 23‐24 April. ● Fellowship provided by the Organization Committee of the SPCE‐TC Society.
2007
17. V Annual meeting of the Center for Neuroscience and Cell Biology, Bencanta, 20‐21 December.
16. VIII European Meeting on Glial Cell in Health and Disease, London, UK, 4‐8 September.
15. 10th Annual meeting of the Portuguese Society for Neurosciences (SPN), Ofir, 24‐26 May.
14. 1st Portugal‐Brazil Coloquium in Neurochemistry, Ofir, 23 May.
13. 2nd Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC), Coimbra, 27‐28 April. ● Fellowship provided by the Organization Committee of the SPCE‐TC Society.
2006
12. IV Annual meeting of the Center for Neuroscience and Cell Biology, Cantanhede, 16‐17 December.
11. XXXVII Annual meeting of the Portuguese Pharmacological Society (SPF), Porto, Portugal, 5‐7 December.
10. 36th Annual Meeting of the Society for Neuroscience, Atlanta, EUA, 14‐18 de October. ● Fellowship provided by the Luso‐American Foundation (FLAD)
9. 1st Annual meeting of the Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC), Funchal, Madeira, 30‐31 May.
2005
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8. XXXVI Annual meeting of the Portuguese Pharmacological Society (SPF), Lisboa, 5‐7 December.
7. 35th Annual Meeting of the Society for Neuroscience, Washington DC, EUA, 12‐16 November. ● Fellowship provided by the Calouste Gulbenkian Foundation
6. III Annual meeting of the Center for Neuroscience and Cell Biology, Bissaya Barreto Institute, Bencanta, 23 September.
5. 20th Biennial Meeting of the International Society for Neurochemistry jointly with the European Society for Neurochemistry, Innsbruck, Austria, 22‐26 de Agosto. ● Fellowship provided by the Organization Committee
4. 9th Annual meeting of the Portuguese Society for Neurosciences (SPN), Luso, 19‐20 May.
3. Molecular mechanisms of neurodegeneration, Milan, Italy, 7‐10 May.
2004
2. 4th FENS Forum of European Neuroscience, Lisboa, 10‐14 July.
1. 7th International NPY meeting, Coimbra, 3‐7 February. 13.2. ADVANCED COURSES ATTENDED
2010
18. Advanced Course: “Regenerative Medicine: The bright and the dark side of Inflammation and Neurogenesis in Epilepsy". Doctoral Programme in Health Sciences, Faculty of Medicine, University of Coimbra, Coimbra, 24‐28 May.
2009
17. Course on Multifluorescence Microscopy, IBILI, Coimbra, 2‐4 September.
16. Advanced Course in Neurogenesis, PDBEB – Doctoral Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology, Coimbra, 23‐27 March.
2008
15. Advanced Course (MIT Portugal Workshop) “New concepts in structural and functional neuroimaging”, IBILI, Coimbra, 18‐20 July.
14. Advanced Course in Neuroimmunology, PDBEB – Doctoral Programme in Experimental Biology and Biomedicine, Center for Neuroscience and Cell Biology, Coimbra, 31 March to 3 April.
2007
13. “VI Course on Animal Manipulation”, Faculty of Veterinary Medicine, Technique University of Lisbon, 19‐30 March.
● Course approved by FELASA (Category C).
2006
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12. Advanced Course (FENS/PENS Summer School) “The Brain as a target for inflammatory processes”, Berlin, Germany, 14‐20 May. ● Fellowship provided by the Organization Committee
11. Advanced Course “Nervous System and Immune System: a struggle and a dialogue”, Braga, 30 January to 2 February.
2005
10. Advanced Course (FENS/Blackwell Summer School) “Neuro‐glia interaction in aging and disease”, Ofir, 5‐10 May.
9. Advanced Course “Proteomics ‐ From sample preparation to protein identification”, IBILI, Coimbra, 28 June to 1 July.
2004
8. Advanced Course “Molecular Neuroscience”, IBILI, Coimbra, 20‐24 October.
7. Advanced Course “Innate Immunity”, “Mario Negri” Institute, Milan, Italy, 24‐27 May.
2003
6. Certificate of the English Language provided by the TECLA School, 2002‐2003.
5. Seminar “O PCR Real Time em tempo real ‐ Expressão genética”, IBILI, Coimbra, 18 March.
2002
4. Seminar “Gestão de competências e valorização pessoal”, FCTUC, Coimbra, 8 ‐11 October.
3. Advanced Course “Gene delivery into Eucariotic cells: From fundamentals to application”, IBILI, Coimbra, 5 ‐7 June.
2. Coloqium Albert Netter “Genética e Biotecnologia em Genecologia ‐ do diagnóstico à terapêutica”, Coimbra, 1‐2 March.
2001
1. Simposium “Horizontes da Genética”, Coimbra, Portugal, 30 November.
14. REFEREE FOR SCIENTIFIC JOURNALS September 2010 – British Journal of Pharmacology
15. OTHER ACTIVITIES 15.1. PARTICIPATION IN MEETINGS ORGANIZATION
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Participation in the organizing committee of the Congress “7th international NPY meeting” realized in the Hotel Melia, Coimbra, 3‐7 February, 2004. 15.2. SCIENCE DIVULGATION
2008
Lecturer at the Science Museum of the University of Coimbra. Talk: “Stem Cells in the adult brain”, Coimbra, 27 November.
2007, 2008, 2009, 2010: Participation in the “Brain Awareness Week”, developing activities in schools and also receiving student at the laboratories. 15.3. MULTIMEDIA Participation in the realization of the 3D movie: Inflammation and neuronal susceptibility to excitotoxic cell death. In: Bernardino L, Malva JO (2007) Interaction between neurons and glia in aging and disease (Malva JO, Rego AC, Cunha RA, Oliveira CR, eds) pp. 3‐36. Springer‐Verlag, New York.
15.4. HOBBIES Swimming, Radical Sports, Gymnasium 16. MEMBERSHIP IN SCIENTIFIC SOCIETIES
1. Federation of European Neuroscience Societies (FENS) 2. Portuguese Society of Biochemistry (SPB) 3. Portuguese Society for Neurosciences (SPN) 4. Portuguese Society for Stem Cells and Cell Therapies (SPCE‐TC)
17. IT SKILLS
Windows (Word, Exel, Powerpoint), WinZip, WinRAR, GraphPad Prism, LSM, AxioVision, Paint Shop Pro, NIH Image 1.62
18. LANGUAGES
Language Reading Writing Conversation English Excelent Excelent Excelent Italian Good Elementar Elementar